Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation

Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication....

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Published in	Emerging infectious diseases Vol. 24; no. 8; pp. 1453 - 1464
Main Authors	Costantini, Veronica, Morantz, Esther K., Browne, Hannah, Ettayebi, Khalil, Zeng, Xi-Lei, Atmar, Robert L., Estes, Mary K., Vinjé, Jan
Format	Journal Article
Language	English
Published	United States U.S. National Center for Infectious Diseases 01.08.2018 Centers for Disease Control and Prevention
Subjects	Alcohols - pharmacology Cell Line Chlorine - pharmacology Health aspects human intestinal enteroids Human Norovirus in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation Humans inactivation Jejunum - cytology Norovirus Norovirus - drug effects Norovirus - physiology replication Small intestine Virus Cultivation Virus inactivation Virus Inactivation - drug effects Virus replication Virus Replication - drug effects Virus Replication - physiology viruses replication viruses virus inactivation norovirus human intestinal enteroids inactivation
Online Access	Get full text
ISSN	1080-6040 1080-6059 1080-6059
DOI	10.3201/eid2408.180126

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Abstract	Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies.
AbstractList	Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies. Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies.Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies.
Audience	Professional Academic
Author	Vinjé, Jan Morantz, Esther K. Zeng, Xi-Lei Estes, Mary K. Atmar, Robert L. Costantini, Veronica Browne, Hannah Ettayebi, Khalil
Author_xml	– sequence: 1 givenname: Veronica surname: Costantini fullname: Costantini, Veronica – sequence: 2 givenname: Esther K. surname: Morantz fullname: Morantz, Esther K. – sequence: 3 givenname: Hannah surname: Browne fullname: Browne, Hannah – sequence: 4 givenname: Khalil surname: Ettayebi fullname: Ettayebi, Khalil – sequence: 5 givenname: Xi-Lei surname: Zeng fullname: Zeng, Xi-Lei – sequence: 6 givenname: Robert L. surname: Atmar fullname: Atmar, Robert L. – sequence: 7 givenname: Mary K. surname: Estes fullname: Estes, Mary K. – sequence: 8 givenname: Jan surname: Vinjé fullname: Vinjé, Jan
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/30014841$$D View this record in MEDLINE/PubMed
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Snippet	Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States....
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SubjectTerms	Alcohols - pharmacology Cell Line Chlorine - pharmacology Health aspects human intestinal enteroids Human Norovirus in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation Humans inactivation Jejunum - cytology Norovirus Norovirus - drug effects Norovirus - physiology replication Small intestine Virus Cultivation Virus inactivation Virus Inactivation - drug effects Virus replication Virus Replication - drug effects Virus Replication - physiology viruses
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Title	Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation
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