Identification and Ultrastructural Characterization of a Novel Nuclear Degradation Complex in Differentiating Lens Fiber Cells

• Published in: PloS one Vol. 11; no. 8; p. e0160785
• Main Authors: Costello, M. Joseph, Brennan, Lisa A., Mohamed, Ashik, Gilliland, Kurt O., Johnsen, Sönke, Kantorow, Marc
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18.08.2016; Public Library of Science (PLoS)
• Subjects: Actin; Analysis; Animals; Apoptosis; Autophagy; Biology; Biology and Life Sciences; Cataracts; Cell cycle; Cell Differentiation; Cell Nucleus - ultrastructure; Chick Embryo; Chromatin; Clathrin; Condensates; Degradation; Electron microscopy; Embryonic development; Engineering and Technology; Hypotheses; Kinases; Lens Capsule, Crystalline - cytology; Lens Capsule, Crystalline - embryology; Lens, Crystalline - cytology; Lens, Crystalline - embryology; Lenses; Light microscopy; Lipid bilayers; Lipids; Lysosomes; Macromolecules; Membrane proteins; Microscopy; Nuclear Envelope - ultrastructure; Nuclear membrane; Nuclei; Nuclei (cytology); Nucleoli; Organelles; Physiology; Protein biosynthesis; Protein synthesis; Proteins; Research and Analysis Methods; Studies; Transmission electron microscopes; Transmission electron microscopy; Florida; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0160785

An unresolved issue in structural biology is how the encapsulated lens removes membranous organelles to carry out its role as a transparent optical element. In this ultrastructural study, we establish a mechanism for nuclear elimination in the developing chick lens during the formation of the organelle-free zone. Day 12-15 chick embryo lenses were examined by high-resolution confocal light microscopy and thin section transmission electron microscopy (TEM) following fixation in 10% formalin and 4% paraformaldehyde, and then processing for confocal or TEM as described previously. Examination of developing fiber cells revealed normal nuclei with dispersed chromatin and clear nucleoli typical of cells in active ribosome production to support protein synthesis. Early signs of nuclear degradation were observed about 300 μm from the lens capsule in Day 15 lenses where the nuclei display irregular nuclear stain and prominent indentations that sometimes contained a previously undescribed macromolecular aggregate attached to the nuclear envelope. We have termed this novel structure the nuclear excisosome. This complex by confocal is closely adherent to the nuclear envelope and by TEM appears to degrade the outer leaflet of the nuclear envelope, then the inner leaflet up to 500 μm depth. The images suggest that the nuclear excisosome separates nuclear membrane proteins from lipids, which then form multilamellar assemblies that stain intensely in confocal and in TEM have 5 nm spacing consistent with pure lipid bilayers. The denuded nucleoplasm then degrades by condensation and loss of structure in the range 600 to 700 μm depth producing pyknotic nuclear remnants. None of these stages display any classic autophagic vesicles or lysosomes associated with nuclei. Uniquely, the origin of the nuclear excisosome is from filopodial-like projections of adjacent lens fiber cells that initially contact, and then appear to fuse with the outer nuclear membrane. These filopodial-like projections appear to be initiated with a clathrin-like coat and driven by an internal actin network. In summary, a specialized cellular organelle, the nuclear excisosome, generated in part by adjacent fiber cells degrades nuclei during fiber cell differentiation and maturation.