Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity
Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)6ASEYDYDDDSDDDDEWD]2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkwor...
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| Published in | PloS one Vol. 11; no. 7; p. e0159111 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
14.07.2016
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1932-6203 1932-6203 |
| DOI | 10.1371/journal.pone.0159111 |
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| Abstract | Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)6ASEYDYDDDSDDDDEWD]2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm. |
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| AbstractList | Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)6ASEYDYDDDSDDDDEWD]2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm.Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)6ASEYDYDDDSDDDDEWD]2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm. Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG).sub.6 ASEYDYDDDSDDDDEWD].sub.2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm. Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG)6ASEYDYDDDSDDDDEWD]2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm. Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG) 6 ASEYDYDDDSDDDDEWD] 2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm. Silk fibers have many inherent properties that are suitable for their use in biomaterials. In this study, the silk fibroin was genetically modified by including a Ca-binding sequence, [(AGSGAG) 6 ASEYDYDDDSDDDDEWD] 2 from shell nacreous matrix protein. It can be produced as fibers by transgenic silkworm. The Ca-binding activity and mineralization of the transgenic silk fibroin were examined in vitro. The results showed that this transgenic silk fibroin had relatively higher Ca-binding activity than unmodified silk fibroin. The increased Ca-binding activity could promote the usage of silk fibroin as a biomaterial in the pharmaceutical industry. This study shows the possibility of using silk fibroin as a mineralization accelerating medical material by generating genetically modified transgenic silkworm. |
| Audience | Academic |
| Author | Gong, Lu Qian, Qiujie Wang, Shaohua Shuai, Yajun You, Zhengying Zhang, Yuyu Zhong, Boxiong Yang, Mingying Chen, Yuyin Ye, Lupeng |
| AuthorAffiliation | 2 College of Life Sciences, Zhejiang University, Hangzhou 310058, P.R. China Institute of Plant Physiology and Ecology, CHINA 1 College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China |
| AuthorAffiliation_xml | – name: Institute of Plant Physiology and Ecology, CHINA – name: 1 College of Animal Sciences, Zhejiang University, Hangzhou 310058, P.R. China – name: 2 College of Life Sciences, Zhejiang University, Hangzhou 310058, P.R. China |
| Author_xml | – sequence: 1 givenname: Shaohua surname: Wang fullname: Wang, Shaohua – sequence: 2 givenname: Yuyu surname: Zhang fullname: Zhang, Yuyu – sequence: 3 givenname: Mingying surname: Yang fullname: Yang, Mingying – sequence: 4 givenname: Lupeng surname: Ye fullname: Ye, Lupeng – sequence: 5 givenname: Lu surname: Gong fullname: Gong, Lu – sequence: 6 givenname: Qiujie surname: Qian fullname: Qian, Qiujie – sequence: 7 givenname: Yajun surname: Shuai fullname: Shuai, Yajun – sequence: 8 givenname: Zhengying surname: You fullname: You, Zhengying – sequence: 9 givenname: Yuyin surname: Chen fullname: Chen, Yuyin – sequence: 10 givenname: Boxiong surname: Zhong fullname: Zhong, Boxiong |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27414647$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1002_bit_28455 crossref_primary_10_1039_D1MA00960E crossref_primary_10_1021_acs_biomac_8b01576 crossref_primary_10_3390_ijms22052709 crossref_primary_10_1021_acs_biomac_4c00188 crossref_primary_10_3389_fbioe_2024_1411550 crossref_primary_10_1016_j_ijbiomac_2021_01_021 crossref_primary_10_1002_cssc_202301549 crossref_primary_10_1002_adhm_202401458 crossref_primary_10_1021_acsbiomaterials_3c01003 |
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| Copyright | COPYRIGHT 2016 Public Library of Science 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2016 Wang et al 2016 Wang et al |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: BZ. Performed the experiments: BZ SW. Analyzed the data: SW. Contributed reagents/materials/analysis tools: BZ SW. Wrote the paper: BZ SW. Performed the silk characteristic analysis: MY YS. Performed the insertion site experiment: YZ. Contributed to the transgenic experiment, silkworm rearing, and samples isolated: LY QQ ZY. Contributed to the western blot experiment: LG. Afforded the experimental materials: YC. |
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| SubjectTerms | Analysis Animal sciences Animals Animals, Genetically Modified Binding Binding sites Biocompatibility Biocompatible Materials Biology and Life Sciences Biomaterials Biomedical materials Bombyx Bombyx mori Calcium Calcium - metabolism Calcium binding proteins Cloning Deoxyribonucleic acid DNA Fibers Fibroins - metabolism Gene expression Genetic modification Genetically modified animals Hydroxyapatite Light Matrix protein Mechanical properties Medical materials Mineralization Peptides Pharmaceutical industry Physical Sciences Plasmids Protein expression Proteins Silk Silk - chemistry Silk fibroin Silkworms Transgenic |
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| Title | Characterization of Transgenic Silkworm Yielded Biomaterials with Calcium-Binding Activity |
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