Method for determination of methane potentials of solid organic waste
A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 °C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular m...
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| Published in | Waste management (Elmsford) Vol. 24; no. 4; pp. 393 - 400 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford
Elsevier Ltd
01.01.2004
New York, NY Elsevier Science |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0956-053X 1879-2456 1879-2456 |
| DOI | 10.1016/j.wasman.2003.09.009 |
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| Abstract | A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 °C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source-separated organic household waste a methane potential of 495 ml CH
4/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH
4/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200–500 ml CH
4/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested. |
|---|---|
| AbstractList | A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 deg. C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source-separated organic household waste a methane potential of 495 ml CH{sub 4}/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH{sub 4}/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200-500 ml CH{sub 4}/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested. A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 degreesC with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source-separated organic household waste a methane potential of 495 ml CH4/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH4/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200-500 ml CH4/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested. Methane potentials of organic solid waste were measured with a laboratory procedure. Trplicate reactors with 10 g of volatile solids were incubated at 55 degree C with 400 ml of inoculum from a thermophilic biogas plant, and methane production was monitored for 50 d by gas chromatographic measurements. A methane potential of 495 ml/g of volatile solids was determined for source-separated organic household waste. A lag phase of several days was observed for fat and oil. For paper bags, starch, and glucose, 63, 84, and 94% of the theoretical methane potential was achieved, respectively. A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 degrees C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source-separated organic household waste a methane potential of 495 ml CH4/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH4/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200-500 ml CH4/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested.A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 degrees C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source-separated organic household waste a methane potential of 495 ml CH4/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH4/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200-500 ml CH4/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested. A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 °C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source-separated organic household waste a methane potential of 495 ml CH 4/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH 4/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200–500 ml CH 4/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested. A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 degree C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source- separated organic household waste a methane potential of 495 ml CH sub(4)/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH sub(4)/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200-500 ml CH sub(4)/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested. A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 degrees C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source-separated organic household waste a methane potential of 495 ml CH4/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH4/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200-500 ml CH4/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested. |
| Author | Mosbæk, Hans Angelidaki, Irini Hansen, Trine L. Jansen, Jes la Cour Schmidt, Jens Ejbye Christensen, Thomas H. Marca, Emilia |
| Author_xml | – sequence: 1 givenname: Trine L. surname: Hansen fullname: Hansen, Trine L. organization: Environment & Resources DTU, Technical University of Denmark, Kongens Lyngby, Denmark – sequence: 2 givenname: Jens Ejbye surname: Schmidt fullname: Schmidt, Jens Ejbye organization: Environment & Resources DTU, Technical University of Denmark, Kongens Lyngby, Denmark – sequence: 3 givenname: Irini surname: Angelidaki fullname: Angelidaki, Irini organization: Environment & Resources DTU, Technical University of Denmark, Kongens Lyngby, Denmark – sequence: 4 givenname: Emilia surname: Marca fullname: Marca, Emilia organization: Environment & Resources DTU, Technical University of Denmark, Kongens Lyngby, Denmark – sequence: 5 givenname: Jes la Cour surname: Jansen fullname: Jansen, Jes la Cour organization: Department of Water and Environmental Technology, Lund Institute of Technology, University of Lund, Lund, Sweden – sequence: 6 givenname: Hans surname: Mosbæk fullname: Mosbæk, Hans organization: Environment & Resources DTU, Technical University of Denmark, Kongens Lyngby, Denmark – sequence: 7 givenname: Thomas H. surname: Christensen fullname: Christensen, Thomas H. email: thc@er.dtu.dk organization: Environment & Resources DTU, Technical University of Denmark, Kongens Lyngby, Denmark |
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| References | (BIB2) 2002 Jansen J.l.C., Spliid H., Hansen T.L., Svärd Å.,d Christensen T.H. (2004). Assessment of sampling and chemical analysis of source-separated organic household waste. Submitted to Waste Management. Owen, Stuckey, Healy, Young, McCarty (BIB12) 1979; 13 Adani, Calcaterra, Malagutti (BIB1) 2001 Angelidaki, Ahring (BIB3) 1992; 37 Owens, Chinoweth (BIB13) 1993; 27 Heerenklage, Stegmann (BIB7) 2001 Harries, Cross, Smith (BIB6) 2001 Juran (BIB10) 1976 Angelidaki, Ahring (BIB4) 1997; 8 ISO 5725-2:1994, Accuracy (trueness and precision) of measurement methods and results—Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method, International Standard Organization. Eleazer, Odle, Wang, Barlaz (BIB5) 1997; 31 (10.1016/j.wasman.2003.09.009_BIB2) 2002 Angelidaki (10.1016/j.wasman.2003.09.009_BIB3) 1992; 37 Owens (10.1016/j.wasman.2003.09.009_BIB13) 1993; 27 Eleazer (10.1016/j.wasman.2003.09.009_BIB5) 1997; 31 10.1016/j.wasman.2003.09.009_BIB9 Angelidaki (10.1016/j.wasman.2003.09.009_BIB4) 1997; 8 10.1016/j.wasman.2003.09.009_BIB8 Adani (10.1016/j.wasman.2003.09.009_BIB1) 2001 Juran (10.1016/j.wasman.2003.09.009_BIB10) 1976 Owen (10.1016/j.wasman.2003.09.009_BIB12) 1979; 13 Heerenklage (10.1016/j.wasman.2003.09.009_BIB7) 2001 Harries (10.1016/j.wasman.2003.09.009_BIB6) 2001 |
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| SubjectTerms | Applied sciences Biogas Bioreactors CELLULOSE Characterization Chemical Engineering Civil Engineering Engineering and Technology Environmental Engineering Environmental Monitoring - methods ENVIRONMENTAL SCIENCES Exact sciences and technology Forecasting Gases - analysis GLUCOSE Kemiteknik Laboratory measurement METHANE Methane - analysis Naturresursteknik OILS Organic Chemicals Organic waste ORGANIC WASTES Pollution PROTEINS QUALITY CONTROL Refuse Disposal - methods Samhällsbyggnadsteknik SENSITIVITY SOLID WASTES STARCH Teknik Temperature Vattenteknik Water Engineering |
| Title | Method for determination of methane potentials of solid organic waste |
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