Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system

The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis . We construct two virus-inducible CRISPR/Cas9 ve...

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Published inGenome Biology Vol. 19; no. 1; p. 197
Main Authors Ji, Xiang, Si, Xiaomin, Zhang, Yi, Zhang, Huawei, Zhang, Feng, Gao, Caixia
Format Journal Article
LanguageEnglish
Published London BioMed Central 15.11.2018
Springer Nature B.V
BMC
Subjects
Animal Genetics and Genomics
Arabidopsis
Beet severe curly top virus
Bioinformatics
Biomedical and Life Sciences
CRISPR
CRISPR-Cas Systems
CRISPR/Cas9 system
Deoxyribonucleic acid
Disease Resistance
DNA
Evolutionary Biology
Geminiviridae
Geminivirus
gene editing
Gene Editing - methods
Gene expression
genetically modified organisms
genome
Genomes
Human Genetics
Immune system
Infections
Insights from Genome Editing
Life Sciences
Microbial Genetics and Genomics
Nicotiana
Nicotiana benthamiana
Off-target
Plant Genetics and Genomics
Plant viruses
Short Report
Transgenic plants
Virology
Virus-inducible
Virus-resistance
Viruses
Geminivirus
Off-target
Virus-resistance
CRISPR/Cas9 system
Virus-inducible
Online AccessGet full text
ISSN1474-760X
1474-7596
1474-760X
DOI10.1186/s13059-018-1580-4

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Abstract The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis . We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays ( Nicotiana benthamiana ) and transgenic lines ( Arabidopsis ). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis . This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
AbstractList The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis . We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays ( Nicotiana benthamiana ) and transgenic lines ( Arabidopsis ). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis . This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
Abstract The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
ArticleNumber 197
Author Zhang, Yi
Si, Xiaomin
Gao, Caixia
Ji, Xiang
Zhang, Huawei
Zhang, Feng
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  surname: Zhang
  fullname: Zhang, Yi
  organization: Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University
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  surname: Zhang
  fullname: Zhang, Huawei
  organization: State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
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  organization: Department of Plant and Microbial Biology, University of Minnesota, Center for Precision Plant Genomics, University of Minnesota
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  surname: Gao
  fullname: Gao, Caixia
  email: cxgao@genetics.ac.cn
  organization: State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences
BackLink https://www.ncbi.nlm.nih.gov/pubmed/30442181$$D View this record in MEDLINE/PubMed
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Issue 1
Keywords Geminivirus
Off-target
Virus-resistance
CRISPR/Cas9 system
Virus-inducible
Language English
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Snippet The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this...
Abstract The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this...
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SubjectTerms Animal Genetics and Genomics
Arabidopsis
Beet severe curly top virus
Bioinformatics
Biomedical and Life Sciences
CRISPR
CRISPR-Cas Systems
CRISPR/Cas9 system
Deoxyribonucleic acid
Disease Resistance
DNA
Evolutionary Biology
Geminiviridae
Geminivirus
gene editing
Gene Editing - methods
Gene expression
genetically modified organisms
genome
Genomes
Human Genetics
Immune system
Infections
Insights from Genome Editing
Life Sciences
Microbial Genetics and Genomics
Nicotiana
Nicotiana benthamiana
Off-target
Plant Genetics and Genomics
Plant viruses
Short Report
Transgenic plants
Virology
Virus-inducible
Virus-resistance
Viruses
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Title Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system
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