Changes in the immunogold electron-microscopic localization of calpain in bovine skeletal muscle induced by conditioning and high-pressure treatment

• Published in: Bioscience, biotechnology, and biochemistry Vol. 70; no. 5; pp. 1249 - 1254
• Main Authors: Borjigin, G.(Niigata Univ. (Japan). Faculty of Agriculture), Yamamoto, S, Sugiyama, T, Nishiumi, T, Suzuki, A
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.05.2006; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Animals; Biological and medical sciences; biotechnology; BOVINA; BOVINAE; calpain; Calpain - analysis; Cattle; electron microscopy; Food Handling - methods; Fundamental and applied biological sciences. Psychology; HIGH PRESSURE TECHNOLOGY; high pressure treatment; immunogold electron-microscopy; Immunohistochemistry; meat conditioning; Meat Products; meat tenderization; MICROSCOPIA; MICROSCOPIE; MICROSCOPY; Microscopy, Electron; MUSCLE; Muscle, Skeletal - chemistry; Muscle, Skeletal - ultrastructure; MUSCLES; MUSCULOS; Pressure; PROTEASAS; PROTEASE; PROTEASES; sarcomeres; skeletal muscle; statistical analysis; TECHNOLOGIE HAUTE PRESSION; TECNOLOGIA ALTA PRESION; Bovine; Enzyme; Cysteine endopeptidases; meat conditioning; Calpain; immunogold electron-microscopy; Electron microscopy; Striated muscle; High pressure; Meat; Peptidases; Vertebrata; high- pressure treatment; Mammalia; Hydrolases; Artiodactyla; Localization; meat tenderization; Ungulata
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.70.1249

Changes in the localization of calpain in conditioned and pressurized bovine skeletal muscles were investigated by immunogold electron-microscopy. In the muscle immediately after thawing (control), the relative distributions of colloidal particles statistically calculated by counting the colloidal particles were about 65% and 35% in the I-band/Z-disk and A-band regions, respectively. In the muscle conditioned for 7 days, distribution of colloidal particles was more than two times greater in both the I-band/Z-disk and A-band regions than in the control muscle. Almost no change in either the absolute concentration or relative distribution of the colloidal particles was detectable during further storage. In the muscle exposed to a pressure of 100 MPa or 200 MPa, slightly more immunogold was detected in the sarcomere than in that of the control muscle. Increasing pressure up to 300 MPa enabled high-density particles to be seen throughout the sarcomere. Conversely, few particles were detected in the sarcomere of the muscle exposed to 400 MPa. These electron-microscopic observations were confirmed from the statistical analysis as with the conditioned muscles. It was clear from the results obtained that the pressure-induced changes in calpain localization were much more pronounced than those from conditioning.