One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system - Bilotti, Katharina; Keep, Sarah; Sikkema, Andrew P.; Pryor, John M.; Kirk, James; Foldes, Katalin; Doyle, Nicole; Wu, Ge; Freimanis, Graham; Dowgier, Giulia; Adeyemi, Oluwapelumi; Tabatabaei, S. Kasra; et al. (PloS one, 2024)
Keywords: Animals; Artificial chromosomes; Assembly; Avian infectious bronchitis; Bacterial infections; Biology; Biology and Life Sciences; Breakpoints; Bronchitis; Coronavirus Infections - veterinary; Coronavirus Infections - virology; DNA; DNA damage; E coli; Escherichia coli; Escherichia coli - genetics; Food security; Food supply; Fragments; Genetics; Genome, Viral; Genomes; Genomic instability; Genomics; Immunization; Infections; Infectious bronchitis virus - genetics; Laboratories; Medicine and Health Sciences; Molecular biology; Mortality; Pathogens; Plasmids; Plasmids - genetics; Population genetics; Poultry; Poultry Diseases - virology; Research and Analysis Methods; Respiratory diseases; Reverse Genetics - methods; Severe acute respiratory syndrome coronavirus 2; Site fidelity; Vaccine development; Vaccines; Viruses; Zoonoses; United Kingdom