IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless...
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| Published in | PloS one Vol. 14; no. 3; p. e0214659 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
28.03.2019
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1932-6203 1932-6203 |
| DOI | 10.1371/journal.pone.0214659 |
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| Abstract | Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance. |
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| AbstractList | Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance. Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance.Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance. |
| Audience | Academic |
| Author | Dormann, Dirk |
| AuthorAffiliation | Microscopy facility, MRC London Institute of Medical Sciences, Faculty of Medicine, Imperial College London, London, United Kingdom Arizona State University, UNITED STATES |
| AuthorAffiliation_xml | – name: Arizona State University, UNITED STATES – name: Microscopy facility, MRC London Institute of Medical Sciences, Faculty of Medicine, Imperial College London, London, United Kingdom |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30921439$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1371/journal.pone.0079879 10.1007/978-1-60761-847-8_17 10.1016/B978-0-12-420138-5.00007-0 10.1111/j.1365-2818.2006.01598.x 10.1002/cyto.a.20314 10.1016/B978-0-12-407761-4.00017-8 10.1038/nprot.2011.336 10.1007/4243_2008_027 10.1016/S0248-4900(03)00078-9 10.1016/j.biocel.2017.06.008 10.1002/cyto.a.20313 |
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| Copyright | COPYRIGHT 2019 Public Library of Science 2019 Dirk Dormann. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2019 Dirk Dormann 2019 Dirk Dormann |
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| SubjectTerms | 3-D printers Archives & records Cellular biology Engineering and Technology Fluorescence Fluorescence microscopy Illumination Imaging Lasers Light Light sources Luminous intensity Measurement Medical research Methods Methyltestosterone Microscopes Microscopy Microscopy, Fluorescence - instrumentation Mobile Applications Physical Sciences Research and Analysis Methods Sensors Smartphones Time Factors |
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| Title | IntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging |
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