Increased Intratumoral Neutrophil in Colorectal Carcinomas Correlates Closely with Malignant Phenotype and Predicts Patients' Adverse Prognosis

• Published in: PloS one Vol. 7; no. 1; p. e30806
• Main Authors: Rao, Hui-Lan, Chen, Jie-Wei, Li, Mei, Xiao, Yong-Bo, Fu, Jia, Zeng, Yi-Xin, Cai, Mu-Yan, Xie, Dan
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.01.2012; Public Library of Science (PLoS)
• Subjects: Analysis; Angiogenesis; Antigens, CD - metabolism; Bone marrow; Cell adhesion & migration; Cell Adhesion Molecules - metabolism; Cell survival; Chen, Wei; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - immunology; Colorectal Neoplasms - pathology; Correlation analysis; Disease; Ethics; Female; Genetic aspects; GPI-Linked Proteins - metabolism; Humans; Immunohistochemistry; Infiltration; Inflammation; Laboratories; Leukocytes (neutrophilic); Liver cancer; Male; Medical prognosis; Medical research; Medicine; Metastases; Metastasis; Middle Aged; Motility; Mucosa; Multivariate Analysis; Neutrophil Infiltration; Neutrophils; Neutrophils - cytology; Neutrophils - immunology; Neutrophils - metabolism; Oncology; Pathology; Patients; Phenotype; Prognosis; Quality; Rodents; Survival; Survival Rate; T-Lymphocytes - immunology; Tissues; Tumorigenesis; Tumors; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0030806

Substantial evidence suggests that the presence of inflammatory cells plays a critical role in the development and/or progression of human tumors. Neutrophils are the common inflammatory cells in tumors; however, the infiltration of intratumoral neutrophils in colorectal carcinoma (CRC) and its effect on CRC patients' prognosis are poorly understood. In this study, the methods of tissue microarray and immunohistochemistry (IHC) were used to investigate the prognostic significance of intratumoral CD66b+ neutrophil in CRC. According to receiver operating characteristic curve analysis, the cutoff score for high intratumoral CD66b+ neutrophil in CRC was defined when the mean counts were more than 60 per TMA spot. In our study, high intratumoral CD66b+ neutrophil was observed in 104/229 (45.4%) of CRCs and in 29/229 (12.7%) of adjacent mucosal tissues. Further correlation analysis showed that high intratumoral neutrophil was positively correlated with pT status, pM status and clinical stage (P<0.05). In univariate survival analysis, a significant association between high intratumoral neutrophil and shortened patients' survival was found (P<0.0001). In different subsets of CRC patients, intratumoral neutrophil was also a prognostic indicator in patients with stage II, stage III, grade 2, grade 3, pT1, pT2, pN0 and pN1 (P<0.05). Importantly, high intratumoral neutrophil was evaluated as an independent prognostic factor in multivariate analysis (P<0.05). Our results provide evidence that increased intratumoral neutrophil in CRC may be important in the acquisition of a malignant phenotype, indicating that the presence of intratumoral neutrophil is an independent factor for poor prognosis of patients with CRC.