Construction and flow cytometric screening of targeted enzyme libraries - Varadarajan, Navin; Cantor, Jason R; Georgiou, George; Iverson, Brent L (Nature protocols, 2009)
Keywords: Analytical Chemistry; Bacterial Outer Membrane Proteins - chemistry; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - metabolism; Biological Techniques; Biomedical and Life Sciences; Catalytic Domain - genetics; Cell Membrane - enzymology; Computational Biology/Bioinformatics; Construction; E coli; Enzymes; Enzymes - chemistry; Enzymes - genetics; Enzymes - metabolism; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Flow cytometry; Flow Cytometry - methods; Fluorescence; Genetic aspects; Genetic engineering; Genomic libraries; Life Sciences; Methods; Microarrays; Models, Molecular; Mutagenesis; Mutation; Organic Chemistry; Peptide Hydrolases - chemistry; Peptide Hydrolases - genetics; Peptide Hydrolases - metabolism; Physiological aspects; Proteases; Protein Engineering; Proteins; Protocol; Residues; Substrate Specificity; Substrates; Synthesis; Testing; United States; United States--US; Texas