Design of multi-epitope peptides containing HLA class-I and class-II-restricted epitopes derived from immunogenic Leishmania proteins, and evaluation of CD4+ and CD8+ T cell responses induced in cured cutaneous leishmaniasis subjects
Human leishmaniasis is a public health problem worldwide for which the development of a vaccine remains a challenge. T cell-mediated immune responses are crucial for protection. Peptide vaccines based on the identification of immunodominant T cell epitopes able to induce T cell specific immune respo...
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| Published in | PLoS neglected tropical diseases Vol. 14; no. 3; p. e0008093 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
01.03.2020
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1935-2735 1935-2727 1935-2735 |
| DOI | 10.1371/journal.pntd.0008093 |
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| Abstract | Human leishmaniasis is a public health problem worldwide for which the development of a vaccine remains a challenge. T cell-mediated immune responses are crucial for protection. Peptide vaccines based on the identification of immunodominant T cell epitopes able to induce T cell specific immune responses constitute a promising strategy. Here, we report the identification of human leukocyte antigen class-I (HLA-I) and -II (HLA-II)-restricted multi-epitope peptides from Leishmania proteins that we have previously described as vaccine candidates. Promastigote Surface Antigen (PSA), LmlRAB (L. major large RAB GTPase) and Histone (H2B) were screened, in silico, for T cell epitopes. 6 HLA-I and 5 HLA-II-restricted multi-epitope peptides, able to bind to the most frequent HLA molecules, were designed and used as pools to stimulate PBMCs from individuals with healed cutaneous leishmaniasis. IFN-γ, IL-10, TNF-α and granzyme B (GrB) production was evaluated by ELISA/CBA. The frequency of IFN-γ-producing T cells was quantified by ELISpot. T cells secreting cytokines and memory T cells were analyzed by flow cytometry. 16 of 25 peptide pools containing HLA-I, HLA-II or HLA-I and -II peptides were able to induce specific and significant IFN-γ levels. No IL-10 was detected. 6 peptide pools were selected among those inducing the highest IFN-γ levels for further characterization. 3/6 pools were able to induce a significant increase of the percentages of CD4+IFN-γ+, CD8+IFN-γ+ and CD4+GrB+ T cells. The same pools also induced a significant increase of the percentages of bifunctional IFN-γ+/TNF-α+CD4+ and/or central memory T cells. We identified highly promiscuous HLA-I and -II restricted epitope combinations from H2B, PSA and LmlRAB proteins that stimulate both CD4+ and CD8+ T cell responses in recovered individuals. These multi-epitope peptides could be used as potential components of a polytope vaccine for human leishmaniasis. |
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| AbstractList | Human leishmaniasis is a public health problem worldwide for which the development of a vaccine remains a challenge. T cell-mediated immune responses are crucial for protection. Peptide vaccines based on the identification of immunodominant T cell epitopes able to induce T cell specific immune responses constitute a promising strategy. Here, we report the identification of human leukocyte antigen class-I (HLA-I) and -II (HLA-II)-restricted multi-epitope peptides from Leishmania proteins that we have previously described as vaccine candidates. Promastigote Surface Antigen (PSA), LmlRAB (L. major large RAB GTPase) and Histone (H2B) were screened, in silico, for T cell epitopes. 6 HLA-I and 5 HLA-II-restricted multi-epitope peptides, able to bind to the most frequent HLA molecules, were designed and used as pools to stimulate PBMCs from individuals with healed cutaneous leishmaniasis. IFN-γ, IL-10, TNF-α and granzyme B (GrB) production was evaluated by ELISA/CBA. The frequency of IFN-γ-producing T cells was quantified by ELISpot. T cells secreting cytokines and memory T cells were analyzed by flow cytometry. 16 of 25 peptide pools containing HLA-I, HLA-II or HLA-I and -II peptides were able to induce specific and significant IFN-γ levels. No IL-10 was detected. 6 peptide pools were selected among those inducing the highest IFN-γ levels for further characterization. 3/6 pools were able to induce a significant increase of the percentages of CD4+IFN-γ+, CD8+IFN-γ+ and CD4+GrB+ T cells. The same pools also induced a significant increase of the percentages of bifunctional IFN-γ+/TNF-α+CD4+ and/or central memory T cells. We identified highly promiscuous HLA-I and -II restricted epitope combinations from H2B, PSA and LmlRAB proteins that stimulate both CD4+ and CD8+ T cell responses in recovered individuals. These multi-epitope peptides could be used as potential components of a polytope vaccine for human leishmaniasis. Human leishmaniasis is a public health problem worldwide for which the development of a vaccine remains a challenge. T cell-mediated immune responses are crucial for protection. Peptide vaccines based on the identification of immunodominant T cell epitopes able to induce T cell specific immune responses constitute a promising strategy. Here, we report the identification of human leukocyte antigen class-I (HLA-I) and -II (HLA-II)-restricted multi-epitope peptides from Leishmania proteins that we have previously described as vaccine candidates. Promastigote Surface Antigen (PSA), LmlRAB ( L . major large RAB GTPase) and Histone (H2B) were screened, in silico , for T cell epitopes. 6 HLA-I and 5 HLA-II-restricted multi-epitope peptides, able to bind to the most frequent HLA molecules, were designed and used as pools to stimulate PBMCs from individuals with healed cutaneous leishmaniasis. IFN-γ, IL-10, TNF-α and granzyme B (GrB) production was evaluated by ELISA/CBA. The frequency of IFN-γ-producing T cells was quantified by ELISpot. T cells secreting cytokines and memory T cells were analyzed by flow cytometry. 16 of 25 peptide pools containing HLA-I, HLA-II or HLA-I and -II peptides were able to induce specific and significant IFN-γ levels. No IL-10 was detected. 6 peptide pools were selected among those inducing the highest IFN-γ levels for further characterization. 3/6 pools were able to induce a significant increase of the percentages of CD4+IFN-γ+, CD8+IFN-γ+ and CD4+GrB+ T cells. The same pools also induced a significant increase of the percentages of bifunctional IFN-γ+/TNF-α+CD4+ and/or central memory T cells. We identified highly promiscuous HLA-I and -II restricted epitope combinations from H2B, PSA and LmlRAB proteins that stimulate both CD4+ and CD8+ T cell responses in recovered individuals. These multi-epitope peptides could be used as potential components of a polytope vaccine for human leishmaniasis. The control of leishmaniasis, a neglected tropical disease of public health importance, caused by protozoan parasites of the genus Leishmania , mainly relies on chemotherapy, which is highly toxic. Currently, there is no vaccine against human leishmaniasis. Peptide-based vaccines consisting of T cell epitopes identified within proteins of interest by epitope predictive algorithms are a promising strategy for vaccine development. Here, we identified multi-epitope peptides composed of HLA-I and -II-restricted epitopes, using immunoinformatic tools, within Leishmania proteins previously described as potential vaccine candidates. We showed that multi-epitope peptides used as pools were able to activate IFN-γ producing CD4+ as well as CD8+ T cells, both required for parasite elimination. In addition, granzyme B-producing CD4+ T cells, bifunctional CD4+ IFN-γ+/TNF-α+ and/or TNF-α+/IL-2+ T cells as well as CD4+ and CD8+ central memory T cells, all involved in Leishmania infection control, were significantly increased in response to multi-epitope peptide stimulation. As far as we know, no study has described the detection of both CD4+ and CD8+ T cell populations in response to stimulation by both HLA-I and II-restricted peptides in humans. The immunogenic HLA-I and -II-restricted multi-epitope peptides identified in this study could constitute potential vaccine candidates against human leishmaniasis. Human leishmaniasis is a public health problem worldwide for which the development of a vaccine remains a challenge. T cell-mediated immune responses are crucial for protection. Peptide vaccines based on the identification of immunodominant T cell epitopes able to induce T cell specific immune responses constitute a promising strategy. Here, we report the identification of human leukocyte antigen class-I (HLA-I) and -II (HLA-II)-restricted multi-epitope peptides from Leishmania proteins that we have previously described as vaccine candidates. Promastigote Surface Antigen (PSA), LmlRAB (L. major large RAB GTPase) and Histone (H2B) were screened, in silico, for T cell epitopes. 6 HLA-I and 5 HLA-II-restricted multi-epitope peptides, able to bind to the most frequent HLA molecules, were designed and used as pools to stimulate PBMCs from individuals with healed cutaneous leishmaniasis. IFN-γ, IL-10, TNF-α and granzyme B (GrB) production was evaluated by ELISA/CBA. The frequency of IFN-γ-producing T cells was quantified by ELISpot. T cells secreting cytokines and memory T cells were analyzed by flow cytometry. 16 of 25 peptide pools containing HLA-I, HLA-II or HLA-I and -II peptides were able to induce specific and significant IFN-γ levels. No IL-10 was detected. 6 peptide pools were selected among those inducing the highest IFN-γ levels for further characterization. 3/6 pools were able to induce a significant increase of the percentages of CD4+IFN-γ+, CD8+IFN-γ+ and CD4+GrB+ T cells. The same pools also induced a significant increase of the percentages of bifunctional IFN-γ+/TNF-α+CD4+ and/or central memory T cells. We identified highly promiscuous HLA-I and -II restricted epitope combinations from H2B, PSA and LmlRAB proteins that stimulate both CD4+ and CD8+ T cell responses in recovered individuals. These multi-epitope peptides could be used as potential components of a polytope vaccine for human leishmaniasis.Human leishmaniasis is a public health problem worldwide for which the development of a vaccine remains a challenge. T cell-mediated immune responses are crucial for protection. Peptide vaccines based on the identification of immunodominant T cell epitopes able to induce T cell specific immune responses constitute a promising strategy. Here, we report the identification of human leukocyte antigen class-I (HLA-I) and -II (HLA-II)-restricted multi-epitope peptides from Leishmania proteins that we have previously described as vaccine candidates. Promastigote Surface Antigen (PSA), LmlRAB (L. major large RAB GTPase) and Histone (H2B) were screened, in silico, for T cell epitopes. 6 HLA-I and 5 HLA-II-restricted multi-epitope peptides, able to bind to the most frequent HLA molecules, were designed and used as pools to stimulate PBMCs from individuals with healed cutaneous leishmaniasis. IFN-γ, IL-10, TNF-α and granzyme B (GrB) production was evaluated by ELISA/CBA. The frequency of IFN-γ-producing T cells was quantified by ELISpot. T cells secreting cytokines and memory T cells were analyzed by flow cytometry. 16 of 25 peptide pools containing HLA-I, HLA-II or HLA-I and -II peptides were able to induce specific and significant IFN-γ levels. No IL-10 was detected. 6 peptide pools were selected among those inducing the highest IFN-γ levels for further characterization. 3/6 pools were able to induce a significant increase of the percentages of CD4+IFN-γ+, CD8+IFN-γ+ and CD4+GrB+ T cells. The same pools also induced a significant increase of the percentages of bifunctional IFN-γ+/TNF-α+CD4+ and/or central memory T cells. We identified highly promiscuous HLA-I and -II restricted epitope combinations from H2B, PSA and LmlRAB proteins that stimulate both CD4+ and CD8+ T cell responses in recovered individuals. These multi-epitope peptides could be used as potential components of a polytope vaccine for human leishmaniasis. Human leishmaniasis is a public health problem worldwide for which the development of a vaccine remains a challenge. T cell-mediated immune responses are crucial for protection. Peptide vaccines based on the identification of immunodominant T cell epitopes able to induce T cell specific immune responses constitute a promising strategy. Here, we report the identification of human leukocyte antigen class-I (HLA-I) and -II (HLA-II)-restricted multi-epitope peptides from Leishmania proteins that we have previously described as vaccine candidates. Promastigote Surface Antigen (PSA), LmlRAB (L. major large RAB GTPase) and Histone (H2B) were screened, in silico, for T cell epitopes. 6 HLA-I and 5 HLA-II-restricted multi-epitope peptides, able to bind to the most frequent HLA molecules, were designed and used as pools to stimulate PBMCs from individuals with healed cutaneous leishmaniasis. IFN-[gamma], IL-10, TNF-[alpha] and granzyme B (GrB) production was evaluated by ELISA/CBA. The frequency of IFN-[gamma]-producing T cells was quantified by ELISpot. T cells secreting cytokines and memory T cells were analyzed by flow cytometry. 16 of 25 peptide pools containing HLA-I, HLA-II or HLA-I and -II peptides were able to induce specific and significant IFN-[gamma] levels. No IL-10 was detected. 6 peptide pools were selected among those inducing the highest IFN-[gamma] levels for further characterization. 3/6 pools were able to induce a significant increase of the percentages of CD4+IFN-[gamma]+, CD8+IFN-[gamma]+ and CD4+GrB+ T cells. The same pools also induced a significant increase of the percentages of bifunctional IFN-[gamma]+/TNF-[alpha]+CD4+ and/or central memory T cells. We identified highly promiscuous HLA-I and -II restricted epitope combinations from H2B, PSA and LmlRAB proteins that stimulate both CD4+ and CD8+ T cell responses in recovered individuals. These multi-epitope peptides could be used as potential components of a polytope vaccine for human leishmaniasis. |
| Audience | Academic |
| Author | Kidar, Abdelhamid Lemesre, Jean-Loup Petitdidier, Elodie Aoun, Karim Messaoudi, Yasmine Pagniez, Julie Meddeb-Garnaoui, Amel Hamrouni, Sarra Chamakh-Ayari, Rym Bras-Gonçalves, Rachel |
| AuthorAffiliation | 4 Hôpital Régional de Gafsa, Gafsa, Tunisia University of Notre Dame, UNITED STATES 3 UMR INTERTRYP, Université de Montpellier, IRD, CIRAD, Montpellier, France 2 Faculté des Sciences de Bizerte, Université de Carthage, Tunis, Tunisie 1 Laboratoire de Parasitologie Médicale, Biotechnologie et Biomolécules, Institut Pasteur de Tunis, Tunis, Tunisie |
| AuthorAffiliation_xml | – name: 1 Laboratoire de Parasitologie Médicale, Biotechnologie et Biomolécules, Institut Pasteur de Tunis, Tunis, Tunisie – name: 4 Hôpital Régional de Gafsa, Gafsa, Tunisia – name: 3 UMR INTERTRYP, Université de Montpellier, IRD, CIRAD, Montpellier, France – name: University of Notre Dame, UNITED STATES – name: 2 Faculté des Sciences de Bizerte, Université de Carthage, Tunis, Tunisie |
| Author_xml | – sequence: 1 givenname: Sarra surname: Hamrouni fullname: Hamrouni, Sarra – sequence: 2 givenname: Rachel orcidid: 0000-0002-3089-4530 surname: Bras-Gonçalves fullname: Bras-Gonçalves, Rachel – sequence: 3 givenname: Abdelhamid surname: Kidar fullname: Kidar, Abdelhamid – sequence: 4 givenname: Karim surname: Aoun fullname: Aoun, Karim – sequence: 5 givenname: Rym surname: Chamakh-Ayari fullname: Chamakh-Ayari, Rym – sequence: 6 givenname: Elodie orcidid: 0000-0002-8554-8424 surname: Petitdidier fullname: Petitdidier, Elodie – sequence: 7 givenname: Yasmine orcidid: 0000-0003-0033-2729 surname: Messaoudi fullname: Messaoudi, Yasmine – sequence: 8 givenname: Julie surname: Pagniez fullname: Pagniez, Julie – sequence: 9 givenname: Jean-Loup orcidid: 0000-0002-7406-8820 surname: Lemesre fullname: Lemesre, Jean-Loup – sequence: 10 givenname: Amel orcidid: 0000-0002-1581-2779 surname: Meddeb-Garnaoui fullname: Meddeb-Garnaoui, Amel |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32176691$$D View this record in MEDLINE/PubMed https://riip.hal.science/pasteur-03557433$$DView record in HAL |
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| CitedBy_id | crossref_primary_10_3390_vaccines9030270 crossref_primary_10_1021_acs_jproteome_0c00405 crossref_primary_10_3390_vaccines11071129 crossref_primary_10_3390_vaccines10020248 crossref_primary_10_1016_j_cellimm_2022_104592 crossref_primary_10_1016_j_jmgm_2024_108759 crossref_primary_10_3389_fchem_2020_601409 crossref_primary_10_3389_fitd_2024_1306429 crossref_primary_10_1093_bib_bbab553 crossref_primary_10_3390_ijms24065972 crossref_primary_10_1016_j_vaccine_2024_126178 |
| Cites_doi | 10.3389/fimmu.2018.01043 10.1371/journal.ppat.1006571 10.1038/nm1108 10.1016/j.micinf.2012.02.006 10.1051/parasite/2019007 10.1128/IAI.63.11.4261-4267.1995 10.1086/382031 10.1371/journal.ppat.1000484 10.1016/j.vaccine.2009.11.075 10.1002/eji.200636783 10.1371/journal.pntd.0001295 10.1155/2014/636039 10.1007/s00251-004-0647-4 10.1016/j.vaccine.2005.01.059 10.3389/fpubh.2014.00165 10.1186/s12879-016-1458-6 10.1128/IAI.70.6.3122-3129.2002 10.1016/S0171-2985(00)80093-5 10.3389/fimmu.2019.00288 10.1016/j.bbrc.2006.01.005 10.3389/fimmu.2016.00327 10.1038/nm1009 10.1093/ije/dym125 10.1086/498042 10.1038/jid.2013.4 10.3389/fimmu.2014.00171 10.1016/j.micinf.2017.03.002 10.1371/journal.pntd.0004614 10.1186/1471-2172-9-1 10.1371/journal.ppat.1004538 10.1128/IAI.68.4.1760-1764.2000 10.3389/fimmu.2014.00268 10.1016/S1473-3099(16)00003-7 10.1111/cei.12787 10.3389/fimmu.2018.00843 10.1111/cei.13074 10.1002/jcb.26190 10.4161/hv.21881 10.1016/j.trsl.2018.05.001 10.1111/pim.12451 10.1016/S0140-6736(18)31204-2 10.1038/nri933 10.1186/1471-2334-13-529 10.1128/IAI.02404-14 10.1155/2013/637649 10.3389/fcimb.2018.00397 10.1007/s10875-012-9788-7 10.4161/hv.6.1.9601 10.3389/fimmu.2019.00724 10.1016/j.coi.2005.04.010 10.3389/fmicb.2016.00467 10.1016/j.cellimm.2016.07.010 10.1111/j.1365-2249.2011.04536.x 10.1084/jem.168.5.1675 10.1038/nrd2224 10.1097/QAD.0b013e32832fae88 10.1046/j.1365-3083.1999.00554.x 10.1016/j.vaccine.2014.04.026 10.1080/14760584.2018.1459191 10.1371/journal.pone.0000725 10.2174/092986708783503249 10.1016/j.meegid.2014.02.017 10.1186/s13071-017-2127-3 10.1111/j.1462-5822.2009.01348.x 10.3389/fimmu.2017.00227 10.1016/S0022-5193(86)80075-3 10.1016/j.vaccine.2005.12.027 10.4049/jimmunol.164.2.900 10.1038/nature01152 10.3389/fcimb.2018.00393 10.1034/j.1399-0039.2002.590601.x 10.1371/journal.pntd.0000845 10.1039/C5SC03892H 10.1016/j.pt.2019.04.002 10.1371/journal.pone.0092708 10.1016/j.vaccine.2004.04.015 10.1186/s12879-014-0653-6 10.4049/jimmunol.167.12.6967 10.1016/S1672-0229(08)60037-6 10.1128/IAI.69.5.3232-3239.2001 10.1080/07391102.2015.1134349 10.1086/324665 10.1200/JCO.2008.16.6462 10.3389/fimmu.2017.00500 10.1038/jid.2014.396 10.1086/515297 10.3389/fimmu.2018.02621 10.1046/j.1365-2249.1999.00844.x 10.1038/nm1592 10.1016/j.vaccine.2011.11.005 10.4049/jimmunol.1201676 10.1111/pim.12359 10.1007/s00251-011-0513-0 10.1371/journal.pntd.0006052 10.1038/nri.2016.72 10.1186/1756-3305-7-386 10.1126/scitranslmed.aac5477 10.1016/j.cellimm.2008.01.001 10.3389/fimmu.2017.01763 10.1084/jem.20061886 10.3390/vaccines2030515 10.1371/journal.pone.0147076 |
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| Copyright | COPYRIGHT 2020 Public Library of Science 2020 Hamrouni et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Attribution 2020 Hamrouni et al 2020 Hamrouni et al |
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| DOI | 10.1371/journal.pntd.0008093 |
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| References | I Naouar (pntd.0008093.ref076) 2016; 11 Z Mou (pntd.0008093.ref099) 2015; 7 J Greenbaum (pntd.0008093.ref059) 2011; 63 S Burza (pntd.0008093.ref001) 2018; 392 K Kemp (pntd.0008093.ref014) 1999; 49 D Sacks (pntd.0008093.ref008) 2002; 2 MV Alves-Silva (pntd.0008093.ref089) 2019; 10 H Kaushal (pntd.0008093.ref032) 2014; 14 L Fernandez (pntd.0008093.ref097) 2018; 9 M Nateghi Rostami (pntd.0008093.ref026) 2010; 4 NC Peters (pntd.0008093.ref071) 2009; 5 E Silva (pntd.0008093.ref084) 2016; 7 I Naouar (pntd.0008093.ref087) 2014; 2014 R Bras-Goncalves (pntd.0008093.ref064) 2014; 24 DS Dias (pntd.0008093.ref085) 2018; 200 LS Hohman (pntd.0008093.ref107) 2019; 35 MV Alves-Silva (pntd.0008093.ref067) 2017; 8 AM Carvalho (pntd.0008093.ref034) 2013; 13 WH Reece (pntd.0008093.ref101) 2004; 10 AS De Groot (pntd.0008093.ref039) 2005; 23 Y Belkaid (pntd.0008093.ref106) 2002; 420 A Egui (pntd.0008093.ref096) 2018; 8 O Launay (pntd.0008093.ref063) 2007; 2 R Chamakh-Ayari (pntd.0008093.ref048) 2017; 10 E Bourreau (pntd.0008093.ref017) 2002; 70 T Bousoffara (pntd.0008093.ref031) 2004; 189 S Ajdary (pntd.0008093.ref010) 2000; 68 A Lanzavecchia (pntd.0008093.ref100) 2005; 17 P Scott (pntd.0008093.ref009) 2016; 16 A Ponte-Sucre (pntd.0008093.ref006) 2017; 11 O Lund (pntd.0008093.ref055) 2004; 55 R Chamakh-Ayari (pntd.0008093.ref047) 2014; 9 A Sassi (pntd.0008093.ref012) 1999; 116 AB Macedo (pntd.0008093.ref021) 2012; 167 TM Cardoso (pntd.0008093.ref030) 2015; 83 CF Anderson (pntd.0008093.ref018) 2007; 204 E Handman (pntd.0008093.ref052) 1995; 63 NC Peters (pntd.0008093.ref098) 2014; 10 SA Calarota (pntd.0008093.ref102) 2013; 2013 M Barve (pntd.0008093.ref042) 2008; 26 A Khamesipour (pntd.0008093.ref036) 2012; 14 MR Dikhit (pntd.0008093.ref082) 2017; 8 H Louzir (pntd.0008093.ref015) 1998; 177 ME Elfaki (pntd.0008093.ref081) 2012; 8 A Sette (pntd.0008093.ref056) 2002; 59 M Skwarczynski (pntd.0008093.ref066) 2016; 7 RCF De Brito (pntd.0008093.ref068) 2018; 9 MR Dikhit (pntd.0008093.ref080) 2018; 119 WR Taylor (pntd.0008093.ref053) 1986; 119 W Baier (pntd.0008093.ref061) 2000; 201 W Li (pntd.0008093.ref046) 2014; 2 N Seyed (pntd.0008093.ref075) 2011; 5 JE Uzonna (pntd.0008093.ref105) 2001; 167 ML Barbosa Santos (pntd.0008093.ref023) 2017; 8 S Iborra (pntd.0008093.ref038) 2018; 17 MR Dikhit (pntd.0008093.ref077) 2017; 39 S Rosendahl Huber (pntd.0008093.ref044) 2014; 5 E Petitdidier (pntd.0008093.ref050) 2016; 10 M Chenik (pntd.0008093.ref070) 2006; 341 S Santos Cda (pntd.0008093.ref027) 2013; 133 PM Moyle (pntd.0008093.ref062) 2008; 15 S Joshi (pntd.0008093.ref083) 2019; 10 FA Castelli (pntd.0008093.ref092) 2007; 37 NC Peters (pntd.0008093.ref072) 2009; 11 VT Martins (pntd.0008093.ref091) 2017; 39 B Saffari (pntd.0008093.ref074) 2009; 7 AM Da-Cruz (pntd.0008093.ref025) 2002; 9 M Chenik (pntd.0008093.ref051) 2006; 24 NC Peters (pntd.0008093.ref073) 2012; 189 C da Silva Santos (pntd.0008093.ref028) 2015; 135 A Levy (pntd.0008093.ref058) 2007; 250 T Boussoffara (pntd.0008093.ref019) 2019; 26 M Hamze (pntd.0008093.ref093) 2017; 8 W Kammoun-Rebai (pntd.0008093.ref011) 2016; 16 TS Lago (pntd.0008093.ref029) 2018; 9 D Nico (pntd.0008093.ref022) 2014; 5 P Scott (pntd.0008093.ref007) 1988; 168 E Nardin (pntd.0008093.ref043) 2010; 6 J Bettaieb (pntd.0008093.ref005) 2014; 7 T Boussoffara (pntd.0008093.ref033) 2018; 8 N Seyed (pntd.0008093.ref041) 2016; 7 AB Salah (pntd.0008093.ref004) 2007; 36 A Meddeb-Garnaoui (pntd.0008093.ref049) 2010; 28 PA Darrah (pntd.0008093.ref020) 2007; 13 S Iborra (pntd.0008093.ref069) 2004; 22 E Loing (pntd.0008093.ref060) 2000; 164 O Courtenay (pntd.0008093.ref002) 2017; 13 MS Duthie (pntd.0008093.ref037) 2012; 30 RL Bottrel (pntd.0008093.ref094) 2001; 69 Vijayamahantesh (pntd.0008093.ref078) 2017; 19 AW Purcell (pntd.0008093.ref065) 2007; 6 PE Kima (pntd.0008093.ref086) 2013; 4 D Nico (pntd.0008093.ref088) 2014; 5 P Kumar (pntd.0008093.ref090) 2018; 191 E Bourreau (pntd.0008093.ref016) 2001; 184 ND Glennie (pntd.0008093.ref104) 2016; 309 H Keshavarz Valian (pntd.0008093.ref035) 2013; 33 J Sidney (pntd.0008093.ref054) 2008; 9 M Agallou (pntd.0008093.ref040) 2014; 5 A Ben Salah (pntd.0008093.ref013) 2005; 192 S Cardinaud (pntd.0008093.ref057) 2009; 23 C da Silva Santos (pntd.0008093.ref024) 2014; 2 H Kaushal (pntd.0008093.ref095) 2016; 185 C Serna (pntd.0008093.ref045) 2014; 32 C Zaph (pntd.0008093.ref103) 2004; 10 A Amit (pntd.0008093.ref079) 2017; 35 C Karimkhani (pntd.0008093.ref003) 2016; 16 |
| References_xml | – volume: 9 start-page: 1043 year: 2018 ident: pntd.0008093.ref068 article-title: Peptide Vaccines for Leishmaniasis publication-title: Front Immunol doi: 10.3389/fimmu.2018.01043 – volume: 13 start-page: e1006571 issue: 10 year: 2017 ident: pntd.0008093.ref002 article-title: Combining epidemiology with basic biology of sand flies, parasites, and hosts to inform leishmaniasis transmission dynamics and control publication-title: PLoS Pathog doi: 10.1371/journal.ppat.1006571 – volume: 10 start-page: 1104 issue: 10 year: 2004 ident: pntd.0008093.ref103 article-title: Central memory T cells mediate long-term immunity to Leishmania major in the absence of persistent parasites publication-title: Nat Med doi: 10.1038/nm1108 – volume: 14 start-page: 702 issue: 9 year: 2012 ident: pntd.0008093.ref036 article-title: Phenotyping of circulating CD8(+) T cell subsets in human cutaneous leishmaniasis publication-title: Microbes Infect doi: 10.1016/j.micinf.2012.02.006 – volume: 26 start-page: 9 year: 2019 ident: pntd.0008093.ref019 article-title: Histological and immunological differences between zoonotic cutaneous leishmaniasis due to Leishmania major and sporadic cutaneous leishmaniasis due to Leishmania infantum publication-title: Parasite doi: 10.1051/parasite/2019007 – volume: 63 start-page: 4261 issue: 11 year: 1995 ident: pntd.0008093.ref052 article-title: Protective vaccination with promastigote surface antigen 2 from Leishmania major is mediated by a TH1 type of immune response publication-title: Infect Immun doi: 10.1128/IAI.63.11.4261-4267.1995 – volume: 189 start-page: 1265 issue: 7 year: 2004 ident: pntd.0008093.ref031 article-title: Analysis of granzyme B activity as a surrogate marker of Leishmania-specific cell-mediated cytotoxicity in zoonotic cutaneous leishmaniasis publication-title: J Infect Dis doi: 10.1086/382031 – volume: 5 start-page: e1000484 issue: 6 year: 2009 ident: pntd.0008093.ref071 article-title: Vector transmission of leishmania abrogates vaccine-induced protective immunity publication-title: PLoS Pathog doi: 10.1371/journal.ppat.1000484 – volume: 28 start-page: 1881 issue: 7 year: 2010 ident: pntd.0008093.ref049 article-title: Cellular and humoral responses induced by Leishmania histone H2B and its divergent and conserved parts in cutaneous and visceral leishmaniasis patients, respectively publication-title: Vaccine doi: 10.1016/j.vaccine.2009.11.075 – volume: 37 start-page: 1513 issue: 6 year: 2007 ident: pntd.0008093.ref092 article-title: Differential capacity of T cell priming in naive donors of promiscuous CD4+ T cell epitopes of HCV NS3 and Core proteins publication-title: Eur J Immunol doi: 10.1002/eji.200636783 – volume: 5 start-page: e1295 issue: 9 year: 2011 ident: pntd.0008093.ref075 article-title: In silico analysis of six known Leishmania major antigens and in vitro evaluation of specific epitopes eliciting HLA-A2 restricted CD8 T cell response publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0001295 – volume: 2014 start-page: 636039 year: 2014 ident: pntd.0008093.ref087 article-title: Involvement of different CD4(+) T cell subsets producing granzyme B in the immune response to Leishmania major antigens publication-title: Mediators Inflamm doi: 10.1155/2014/636039 – volume: 55 start-page: 797 issue: 12 year: 2004 ident: pntd.0008093.ref055 article-title: Definition of supertypes for HLA molecules using clustering of specificity matrices publication-title: Immunogenetics doi: 10.1007/s00251-004-0647-4 – volume: 23 start-page: 2121 issue: 17–18 year: 2005 ident: pntd.0008093.ref039 article-title: Developing an epitope-driven tuberculosis (TB) vaccine publication-title: Vaccine doi: 10.1016/j.vaccine.2005.01.059 – volume: 2 start-page: 165 year: 2014 ident: pntd.0008093.ref024 article-title: The Role of CD4 and CD8 T Cells in Human Cutaneous Leishmaniasis publication-title: Front Public Health doi: 10.3389/fpubh.2014.00165 – volume: 16 start-page: 138 year: 2016 ident: pntd.0008093.ref011 article-title: Protein biomarkers discriminate Leishmania major-infected and non-infected individuals in areas endemic for cutaneous leishmaniasis publication-title: BMC Infect Dis doi: 10.1186/s12879-016-1458-6 – volume: 70 start-page: 3122 issue: 6 year: 2002 ident: pntd.0008093.ref017 article-title: LACK-specific CD4(+) T cells that induce gamma interferon production in patients with localized cutaneous leishmaniasis during an early stage of infection publication-title: Infect Immun doi: 10.1128/IAI.70.6.3122-3129.2002 – volume: 201 start-page: 391 issue: 3–4 year: 2000 ident: pntd.0008093.ref061 article-title: Lipopeptides as immunoadjuvants and immunostimulants in mucosal immunization publication-title: Immunobiology doi: 10.1016/S0171-2985(00)80093-5 – volume: 10 start-page: 288 year: 2019 ident: pntd.0008093.ref083 article-title: Immunogenicity and Protective Efficacy of T-Cell Epitopes Derived From Potential Th1 Stimulatory Proteins of Leishmania (Leishmania) donovani publication-title: Front Immunol doi: 10.3389/fimmu.2019.00288 – volume: 341 start-page: 541 issue: 2 year: 2006 ident: pntd.0008093.ref070 article-title: Identification of a new developmentally regulated Leishmania major large RAB GTPase publication-title: Biochem Biophys Res Commun doi: 10.1016/j.bbrc.2006.01.005 – volume: 7 start-page: 327 year: 2016 ident: pntd.0008093.ref084 article-title: Combination of In Silico Methods in the Search for Potential CD4(+) and CD8(+) T Cell Epitopes in the Proteome of Leishmania braziliensis publication-title: Front Immunol doi: 10.3389/fimmu.2016.00327 – volume: 10 start-page: 406 issue: 4 year: 2004 ident: pntd.0008093.ref101 article-title: A CD4(+) T-cell immune response to a conserved epitope in the circumsporozoite protein correlates with protection from natural Plasmodium falciparum infection and disease publication-title: Nat Med doi: 10.1038/nm1009 – volume: 36 start-page: 991 issue: 5 year: 2007 ident: pntd.0008093.ref004 article-title: Zoonotic cutaneous leishmaniasis in central Tunisia: spatio temporal dynamics publication-title: Int J Epidemiol doi: 10.1093/ije/dym125 – volume: 192 start-page: 1981 issue: 11 year: 2005 ident: pntd.0008093.ref013 article-title: The predictive validity of naturally acquired delayed-type hypersensitivity to leishmanin in resistance to Leishmania major-associated cutaneous leishmaniasis publication-title: J Infect Dis doi: 10.1086/498042 – volume: 133 start-page: 1533 issue: 6 year: 2013 ident: pntd.0008093.ref027 article-title: CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNgamma(+)-mediated parasite killing in human cutaneous leishmaniasis publication-title: J Invest Dermatol doi: 10.1038/jid.2013.4 – volume: 5 start-page: 171 year: 2014 ident: pntd.0008093.ref044 article-title: T cell responses to viral infections—opportunities for Peptide vaccination publication-title: Front Immunol doi: 10.3389/fimmu.2014.00171 – volume: 19 start-page: 358 issue: 6 year: 2017 ident: pntd.0008093.ref078 article-title: Immuno-informatics based approaches to identify CD8+ T cell epitopes within the Leishmania donovani 3-ectonucleotidase in cured visceral leishmaniasis subjects publication-title: Microbes Infect doi: 10.1016/j.micinf.2017.03.002 – volume: 10 start-page: e0004614 issue: 5 year: 2016 ident: pntd.0008093.ref050 article-title: Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0004614 – volume: 9 start-page: 1 year: 2008 ident: pntd.0008093.ref054 article-title: HLA class I supertypes: a revised and updated classification publication-title: BMC Immunol doi: 10.1186/1471-2172-9-1 – volume: 10 start-page: e1004538 issue: 12 year: 2014 ident: pntd.0008093.ref098 article-title: Chronic parasitic infection maintains high frequencies of short-lived Ly6C+CD4+ effector T cells that are required for protection against re-infection publication-title: PLoS Pathog doi: 10.1371/journal.ppat.1004538 – volume: 68 start-page: 1760 issue: 4 year: 2000 ident: pntd.0008093.ref010 article-title: Comparison of the immune profile of nonhealing cutaneous Leishmaniasis patients with those with active lesions and those who have recovered from infection publication-title: Infect Immun doi: 10.1128/IAI.68.4.1760-1764.2000 – volume: 5 start-page: 268 year: 2014 ident: pntd.0008093.ref040 article-title: Experimental Validation of Multi-Epitope Peptides Including Promising MHC Class I- and II-Restricted Epitopes of Four Known Leishmania infantum Proteins publication-title: Front Immunol doi: 10.3389/fimmu.2014.00268 – volume: 16 start-page: 584 issue: 5 year: 2016 ident: pntd.0008093.ref003 article-title: Global burden of cutaneous leishmaniasis: a cross-sectional analysis from the Global Burden of Disease Study 2013 publication-title: Lancet Infect Dis doi: 10.1016/S1473-3099(16)00003-7 – volume: 185 start-page: 50 issue: 1 year: 2016 ident: pntd.0008093.ref095 article-title: Evaluation of cellular immunological responses in mono- and polymorphic clinical forms of post-kala-azar dermal leishmaniasis in India publication-title: Clin Exp Immunol doi: 10.1111/cei.12787 – volume: 8 start-page: 100 year: 2017 ident: pntd.0008093.ref067 article-title: A Chimera Containing CD4+ and CD8+ T-Cell Epitopes of the Leishmania donovani Nucleoside Hydrolase (NH36) Optimizes Cross-Protection against Leishmania amazonesis Infection publication-title: Front Immunol – volume: 9 start-page: 843 year: 2018 ident: pntd.0008093.ref097 article-title: Antigenicity of Leishmania-Activated C-Kinase Antigen (LACK) in Human Peripheral Blood Mononuclear Cells, and Protective Effect of Prime-Boost Vaccination With pCI-neo-LACK Plus Attenuated LACK-Expressing Vaccinia Viruses in Hamsters publication-title: Front Immunol doi: 10.3389/fimmu.2018.00843 – volume: 191 start-page: 318 issue: 3 year: 2018 ident: pntd.0008093.ref090 article-title: T cell suppression in the bone marrow of visceral leishmaniasis patients: impact of parasite load publication-title: Clin Exp Immunol doi: 10.1111/cei.13074 – volume: 119 start-page: 378 issue: 1 year: 2018 ident: pntd.0008093.ref080 article-title: Mining the Proteome of Leishmania donovani for the Development of Novel MHC Class I Restricted Epitope for the Control of Visceral Leishmaniasis publication-title: J Cell Biochem doi: 10.1002/jcb.26190 – volume: 8 start-page: 1769 issue: 12 year: 2012 ident: pntd.0008093.ref081 article-title: Immunogenicity and immune modulatory effects of in silico predicted L. donovani candidate peptide vaccines publication-title: Hum Vaccin Immunother doi: 10.4161/hv.21881 – volume: 200 start-page: 18 year: 2018 ident: pntd.0008093.ref085 article-title: Vaccination with a CD4(+) and CD8(+) T-cell epitopes-based recombinant chimeric protein derived from Leishmania infantum proteins confers protective immunity against visceral leishmaniasis publication-title: Transl Res doi: 10.1016/j.trsl.2018.05.001 – volume: 39 issue: 9 year: 2017 ident: pntd.0008093.ref077 article-title: Vaccine potential of HLA-A2 epitopes from Leishmania Cysteine Protease Type III (CPC) publication-title: Parasite Immunol doi: 10.1111/pim.12451 – volume: 392 start-page: 951 issue: 10151 year: 2018 ident: pntd.0008093.ref001 article-title: Leishmaniasis publication-title: Lancet doi: 10.1016/S0140-6736(18)31204-2 – volume: 2 start-page: 845 issue: 11 year: 2002 ident: pntd.0008093.ref008 article-title: The immunology of susceptibility and resistance to Leishmania major in mice publication-title: Nat Rev Immunol doi: 10.1038/nri933 – volume: 13 start-page: 529 year: 2013 ident: pntd.0008093.ref034 article-title: Immunologic response and memory T cells in subjects cured of tegumentary leishmaniasis publication-title: BMC Infect Dis doi: 10.1186/1471-2334-13-529 – volume: 83 start-page: 898 issue: 3 year: 2015 ident: pntd.0008093.ref030 article-title: Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection publication-title: Infect Immun doi: 10.1128/IAI.02404-14 – volume: 2013 start-page: 637649 year: 2013 ident: pntd.0008093.ref102 article-title: Enumeration and characterization of human memory T cells by enzyme-linked immunospot assays publication-title: Clin Dev Immunol doi: 10.1155/2013/637649 – volume: 8 start-page: 397 year: 2018 ident: pntd.0008093.ref033 article-title: Immunity Against Leishmania major Infection: Parasite-Specific Granzyme B Induction as a Correlate of Protection publication-title: Front Cell Infect Microbiol doi: 10.3389/fcimb.2018.00397 – volume: 33 start-page: 220 issue: 1 year: 2013 ident: pntd.0008093.ref035 article-title: CCR7+ central and CCR7- effector memory CD4+ T cells in human cutaneous leishmaniasis publication-title: J Clin Immunol doi: 10.1007/s10875-012-9788-7 – volume: 6 start-page: 27 issue: 1 year: 2010 ident: pntd.0008093.ref043 article-title: The past decade in malaria synthetic peptide vaccine clinical trials publication-title: Hum Vaccin doi: 10.4161/hv.6.1.9601 – volume: 10 start-page: 724 year: 2019 ident: pntd.0008093.ref089 article-title: The F1F3 Recombinant Chimera of Leishmania donovani-Nucleoside Hydrolase (NH36) and Its Epitopes Induce Cross-Protection Against Leishmania (V.) braziliensis Infection in Mice publication-title: Front Immunol doi: 10.3389/fimmu.2019.00724 – volume: 17 start-page: 326 issue: 3 year: 2005 ident: pntd.0008093.ref100 article-title: Understanding the generation and function of memory T cell subsets publication-title: Curr Opin Immunol doi: 10.1016/j.coi.2005.04.010 – volume: 7 start-page: 467 year: 2016 ident: pntd.0008093.ref041 article-title: Post-Genomics and Vaccine Improvement for Leishmania publication-title: Front Microbiol doi: 10.3389/fmicb.2016.00467 – volume: 309 start-page: 50 year: 2016 ident: pntd.0008093.ref104 article-title: Memory T cells in cutaneous leishmaniasis publication-title: Cell Immunol doi: 10.1016/j.cellimm.2016.07.010 – volume: 167 start-page: 505 issue: 3 year: 2012 ident: pntd.0008093.ref021 article-title: Multifunctional CD4(+) T cells in patients with American cutaneous leishmaniasis publication-title: Clin Exp Immunol doi: 10.1111/j.1365-2249.2011.04536.x – volume: 168 start-page: 1675 issue: 5 year: 1988 ident: pntd.0008093.ref007 article-title: Immunoregulation of cutaneous leishmaniasis. T cell lines that transfer protective immunity or exacerbation belong to different T helper subsets and respond to distinct parasite antigens publication-title: J Exp Med doi: 10.1084/jem.168.5.1675 – volume: 9 start-page: 251 issue: 2 year: 2002 ident: pntd.0008093.ref025 article-title: T-cell-mediated immune responses in patients with cutaneous or mucosal leishmaniasis: long-term evaluation after therapy publication-title: Clin Diagn Lab Immunol – volume: 6 start-page: 404 issue: 5 year: 2007 ident: pntd.0008093.ref065 article-title: More than one reason to rethink the use of peptides in vaccine design publication-title: Nat Rev Drug Discov doi: 10.1038/nrd2224 – volume: 23 start-page: 1945 issue: 15 year: 2009 ident: pntd.0008093.ref057 article-title: Design of a HIV-1-derived HLA-B07.02-restricted polyepitope construct publication-title: AIDS doi: 10.1097/QAD.0b013e32832fae88 – volume: 49 start-page: 655 issue: 6 year: 1999 ident: pntd.0008093.ref014 article-title: Interferon-gamma- and tumour necrosis factor-alpha-producing cells in humans who are immune to cutaneous leishmaniasis publication-title: Scand J Immunol doi: 10.1046/j.1365-3083.1999.00554.x – volume: 32 start-page: 3525 issue: 28 year: 2014 ident: pntd.0008093.ref045 article-title: A synthetic peptide from Trypanosoma cruzi mucin-like associated surface protein as candidate for a vaccine against Chagas disease publication-title: Vaccine doi: 10.1016/j.vaccine.2014.04.026 – volume: 17 start-page: 323 issue: 4 year: 2018 ident: pntd.0008093.ref038 article-title: Vaccine candidates against leishmania under current research publication-title: Expert Rev Vaccines doi: 10.1080/14760584.2018.1459191 – volume: 2 start-page: e725 issue: 8 year: 2007 ident: pntd.0008093.ref063 article-title: Cellular immune responses induced with dose-sparing intradermal administration of HIV vaccine to HIV-uninfected volunteers in the ANRS VAC16 trial publication-title: PLoS One doi: 10.1371/journal.pone.0000725 – volume: 5 start-page: 189 year: 2014 ident: pntd.0008093.ref088 article-title: Cross-Protective Immunity to Leishmania amazonensis is Mediated by CD4+ and CD8+ Epitopes of Leishmania donovani Nucleoside Hydrolase Terminal Domains publication-title: Front Immunol – volume: 15 start-page: 506 issue: 5 year: 2008 ident: pntd.0008093.ref062 article-title: Self-adjuvanting lipopeptide vaccines publication-title: Curr Med Chem doi: 10.2174/092986708783503249 – volume: 24 start-page: 1 year: 2014 ident: pntd.0008093.ref064 article-title: Identification and characterization of new Leishmania promastigote surface antigens, LaPSA-38S and LiPSA-50S, as major immunodominant excreted/secreted components of L. amazonensis and L. infantum publication-title: Infect Genet Evol doi: 10.1016/j.meegid.2014.02.017 – volume: 10 start-page: 185 issue: 1 year: 2017 ident: pntd.0008093.ref048 article-title: Leishmania major large RAB GTPase is highly immunogenic in individuals immune to cutaneous and visceral leishmaniasis publication-title: Parasit Vectors doi: 10.1186/s13071-017-2127-3 – volume: 11 start-page: 1290 issue: 9 year: 2009 ident: pntd.0008093.ref072 article-title: The impact of vector-mediated neutrophil recruitment on cutaneous leishmaniasis publication-title: Cell Microbiol doi: 10.1111/j.1462-5822.2009.01348.x – volume: 8 start-page: 227 year: 2017 ident: pntd.0008093.ref023 article-title: Leishmania donovani Nucleoside Hydrolase (NH36) Domains Induce T-Cell Cytokine Responses in Human Visceral Leishmaniasis publication-title: Front Immunol doi: 10.3389/fimmu.2017.00227 – volume: 119 start-page: 205 issue: 2 year: 1986 ident: pntd.0008093.ref053 article-title: The classification of amino acid conservation publication-title: J Theor Biol doi: 10.1016/S0022-5193(86)80075-3 – volume: 24 start-page: 2521 issue: 14 year: 2006 ident: pntd.0008093.ref051 article-title: Vaccination with the divergent portion of the protein histone H2B of Leishmania protects susceptible BALB/c mice against a virulent challenge with Leishmania major publication-title: Vaccine doi: 10.1016/j.vaccine.2005.12.027 – volume: 164 start-page: 900 issue: 2 year: 2000 ident: pntd.0008093.ref060 article-title: Extension of HLA-A*0201-restricted minimal epitope by N epsilon-palmitoyl-lysine increases the life span of functional presentation to cytotoxic T cells publication-title: J Immunol doi: 10.4049/jimmunol.164.2.900 – volume: 5 start-page: 273 year: 2014 ident: pntd.0008093.ref022 article-title: Leishmania donovani Nucleoside Hydrolase Terminal Domains in Cross-Protective Immunotherapy Against Leishmania amazonensis Murine Infection publication-title: Front Immunol – volume: 420 start-page: 502 issue: 6915 year: 2002 ident: pntd.0008093.ref106 article-title: CD4+CD25+ regulatory T cells control Leishmania major persistence and immunity publication-title: Nature doi: 10.1038/nature01152 – volume: 8 start-page: 393 year: 2018 ident: pntd.0008093.ref096 article-title: Phenotypic and Functional Profiles of Antigen-Specific CD4(+) and CD8(+) T Cells Associated With Infection Control in Patients With Cutaneous Leishmaniasis publication-title: Front Cell Infect Microbiol doi: 10.3389/fcimb.2018.00393 – volume: 59 start-page: 443 issue: 6 year: 2002 ident: pntd.0008093.ref056 article-title: Optimizing vaccine design for cellular processing, MHC binding and TCR recognition publication-title: Tissue Antigens doi: 10.1034/j.1399-0039.2002.590601.x – volume: 4 start-page: e845 issue: 10 year: 2010 ident: pntd.0008093.ref026 article-title: CD8+ T cells as a source of IFN-gamma production in human cutaneous leishmaniasis publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0000845 – volume: 7 start-page: 842 issue: 2 year: 2016 ident: pntd.0008093.ref066 article-title: Peptide-based synthetic vaccines publication-title: Chem Sci doi: 10.1039/C5SC03892H – volume: 4 start-page: 156 year: 2013 ident: pntd.0008093.ref086 article-title: Interferon gamma in leishmaniasis publication-title: Front Immunol – volume: 35 start-page: 423 issue: 6 year: 2019 ident: pntd.0008093.ref107 article-title: CD4(+) T Cell-Mediated Immunity against the Phagosomal Pathogen Leishmania: Implications for Vaccination publication-title: Trends Parasitol doi: 10.1016/j.pt.2019.04.002 – volume: 9 start-page: e92708 issue: 5 year: 2014 ident: pntd.0008093.ref047 article-title: In vitro evaluation of a soluble Leishmania promastigote surface antigen as a potential vaccine candidate against human leishmaniasis publication-title: PLoS One doi: 10.1371/journal.pone.0092708 – volume: 22 start-page: 3865 issue: 29–30 year: 2004 ident: pntd.0008093.ref069 article-title: Vaccination with a plasmid DNA cocktail encoding the nucleosomal histones of Leishmania confers protection against murine cutaneous leishmaniosis publication-title: Vaccine doi: 10.1016/j.vaccine.2004.04.015 – volume: 14 start-page: 653 year: 2014 ident: pntd.0008093.ref032 article-title: Role of CD8(+) T cells in protection against Leishmania donovani infection in healed Visceral Leishmaniasis individuals publication-title: BMC Infect Dis doi: 10.1186/s12879-014-0653-6 – volume: 167 start-page: 6967 issue: 12 year: 2001 ident: pntd.0008093.ref105 article-title: Immune elimination of Leishmania major in mice: implications for immune memory, vaccination, and reactivation disease publication-title: J Immunol doi: 10.4049/jimmunol.167.12.6967 – volume: 7 start-page: 87 issue: 3 year: 2009 ident: pntd.0008093.ref074 article-title: Computational analysis of cysteine proteases (Clan CA, Family Cl) of Leishmania major to find potential epitopic regions publication-title: Genomics Proteomics Bioinformatics doi: 10.1016/S1672-0229(08)60037-6 – volume: 69 start-page: 3232 issue: 5 year: 2001 ident: pntd.0008093.ref094 article-title: Flow cytometric determination of cellular sources and frequencies of key cytokine-producing lymphocytes directed against recombinant LACK and soluble Leishmania antigen in human cutaneous leishmaniasis publication-title: Infect Immun doi: 10.1128/IAI.69.5.3232-3239.2001 – volume: 35 start-page: 128 issue: 1 year: 2017 ident: pntd.0008093.ref079 article-title: Immunomodulation mediated through Leishmania donovani protein disulfide isomerase by eliciting CD8+ T-cell in cured visceral leishmaniasis subjects and identification of its possible HLA class-1 restricted T-cell epitopes publication-title: J Biomol Struct Dyn doi: 10.1080/07391102.2015.1134349 – volume: 184 start-page: 1628 issue: 12 year: 2001 ident: pntd.0008093.ref016 article-title: High intralesional interleukin-10 messenger RNA expression in localized cutaneous leishmaniasis is associated with unresponsiveness to treatment publication-title: J Infect Dis doi: 10.1086/324665 – volume: 26 start-page: 4418 issue: 27 year: 2008 ident: pntd.0008093.ref042 article-title: Induction of immune responses and clinical efficacy in a phase II trial of IDM-2101, a 10-epitope cytotoxic T-lymphocyte vaccine, in metastatic non-small-cell lung cancer publication-title: J Clin Oncol doi: 10.1200/JCO.2008.16.6462 – volume: 8 start-page: 500 year: 2017 ident: pntd.0008093.ref093 article-title: Characterization of CD4 T Cell Epitopes of Infliximab and Rituximab Identified from Healthy Donors publication-title: Front Immunol doi: 10.3389/fimmu.2017.00500 – volume: 135 start-page: 400 issue: 2 year: 2015 ident: pntd.0008093.ref028 article-title: Proteome profiling of human cutaneous leishmaniasis lesion publication-title: J Invest Dermatol doi: 10.1038/jid.2014.396 – volume: 177 start-page: 1687 issue: 6 year: 1998 ident: pntd.0008093.ref015 article-title: Immunologic determinants of disease evolution in localized cutaneous leishmaniasis due to Leishmania major publication-title: J Infect Dis doi: 10.1086/515297 – volume: 9 start-page: 2621 year: 2018 ident: pntd.0008093.ref029 article-title: The miRNA 361-3p, a Regulator of GZMB and TNF Is Associated With Therapeutic Failure and Longer Time Healing of Cutaneous Leishmaniasis Caused by L. (viannia) braziliensis publication-title: Front Immunol doi: 10.3389/fimmu.2018.02621 – volume: 116 start-page: 127 issue: 1 year: 1999 ident: pntd.0008093.ref012 article-title: Leishmanin skin test lymphoproliferative responses and cytokine production after symptomatic or asymptomatic Leishmania major infection in Tunisia publication-title: Clin Exp Immunol doi: 10.1046/j.1365-2249.1999.00844.x – volume: 13 start-page: 843 issue: 7 year: 2007 ident: pntd.0008093.ref020 article-title: Multifunctional TH1 cells define a correlate of vaccine-mediated protection against Leishmania major publication-title: Nat Med doi: 10.1038/nm1592 – volume: 30 start-page: 134 issue: 2 year: 2012 ident: pntd.0008093.ref037 article-title: The development and clinical evaluation of second-generation leishmaniasis vaccines publication-title: Vaccine doi: 10.1016/j.vaccine.2011.11.005 – volume: 189 start-page: 4832 issue: 10 year: 2012 ident: pntd.0008093.ref073 article-title: Evaluation of recombinant Leishmania polyprotein plus glucopyranosyl lipid A stable emulsion vaccines against sand fly-transmitted Leishmania major in C57BL/6 mice publication-title: J Immunol doi: 10.4049/jimmunol.1201676 – volume: 39 issue: 1 year: 2017 ident: pntd.0008093.ref091 article-title: A recombinant chimeric protein composed of human and mice-specific CD4(+) and CD8(+) T-cell epitopes protects against visceral leishmaniasis publication-title: Parasite Immunol doi: 10.1111/pim.12359 – volume: 63 start-page: 325 issue: 6 year: 2011 ident: pntd.0008093.ref059 article-title: Functional classification of class II human leukocyte antigen (HLA) molecules reveals seven different supertypes and a surprising degree of repertoire sharing across supertypes publication-title: Immunogenetics doi: 10.1007/s00251-011-0513-0 – volume: 11 start-page: e0006052 issue: 12 year: 2017 ident: pntd.0008093.ref006 article-title: Drug resistance and treatment failure in leishmaniasis: A 21st century challenge publication-title: PLoS Negl Trop Dis doi: 10.1371/journal.pntd.0006052 – volume: 16 start-page: 581 issue: 9 year: 2016 ident: pntd.0008093.ref009 article-title: Cutaneous leishmaniasis: immune responses in protection and pathogenesis publication-title: Nat Rev Immunol doi: 10.1038/nri.2016.72 – volume: 7 start-page: 386 year: 2014 ident: pntd.0008093.ref005 article-title: Prevalence and determinants of Leishmania major infection in emerging and old foci in Tunisia publication-title: Parasit Vectors doi: 10.1186/1756-3305-7-386 – volume: 7 start-page: 310ra167 issue: 310 year: 2015 ident: pntd.0008093.ref099 article-title: Identification of broadly conserved cross-species protective Leishmania antigen and its responding CD4+ T cells publication-title: Sci Transl Med doi: 10.1126/scitranslmed.aac5477 – volume: 250 start-page: 24 issue: 1–2 year: 2007 ident: pntd.0008093.ref058 article-title: A melanoma multiepitope polypeptide induces specific CD8+ T-cell response publication-title: Cell Immunol doi: 10.1016/j.cellimm.2008.01.001 – volume: 8 start-page: 1763 year: 2017 ident: pntd.0008093.ref082 article-title: Identification of Potential MHC Class-II-Restricted Epitopes Derived from Leishmania donovani Antigens by Reverse Vaccinology and Evaluation of Their CD4+ T-Cell Responsiveness against Visceral Leishmaniasis publication-title: Front Immunol doi: 10.3389/fimmu.2017.01763 – volume: 204 start-page: 285 issue: 2 year: 2007 ident: pntd.0008093.ref018 article-title: CD4(+)CD25(-)Foxp3(-) Th1 cells are the source of IL-10-mediated immune suppression in chronic cutaneous leishmaniasis publication-title: J Exp Med doi: 10.1084/jem.20061886 – volume: 2 start-page: 515 issue: 3 year: 2014 ident: pntd.0008093.ref046 article-title: Peptide Vaccine: Progress and Challenges publication-title: Vaccines (Basel) doi: 10.3390/vaccines2030515 – volume: 11 start-page: e0147076 issue: 1 year: 2016 ident: pntd.0008093.ref076 article-title: Prediction of T Cell Epitopes from Leishmania major Potentially Excreted/Secreted Proteins Inducing Granzyme B Production publication-title: PLoS One doi: 10.1371/journal.pone.0147076 |
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| SubjectTerms | Adult Antigenic determinants Antigens Antigens, Protozoan Antigens, Protozoan - genetics Antigens, Protozoan - immunology Biology and Life Sciences CD4 antigen CD4-Positive T-Lymphocytes CD4-Positive T-Lymphocytes - immunology CD8 antigen CD8-Positive T-Lymphocytes CD8-Positive T-Lymphocytes - immunology Cells Cutaneous leishmaniasis Cytokines Deoxyribonucleic acid Disease control DNA ELISA Enzyme-Linked Immunosorbent Assay Epitopes Epitopes, T-Lymphocyte Epitopes, T-Lymphocyte - genetics Epitopes, T-Lymphocyte - immunology Female Flow Cytometry Genetic testing Granzyme B Granzymes Granzymes - analysis Guanosine triphosphatases Health aspects Histocompatibility antigen HLA Histocompatibility Antigens Class I Histocompatibility Antigens Class I - metabolism Histocompatibility Antigens Class II Histocompatibility Antigens Class II - metabolism Histones HLA antigens Humans Identification Immune response (cell-mediated) Immune system Immunodominance Immunogenicity Immunological memory Infections Interferon-gamma Interferon-gamma - analysis Interleukin 10 Interleukin-10 - analysis Leishmania Leishmania - immunology Leishmaniasis, Cutaneous Leishmaniasis, Cutaneous - immunology Leukocytes Life Sciences Lymphocytes Lymphocytes T Male Medicine and Health Sciences Memory cells Middle Aged Molecules Parasites Parasitic diseases Peptides Pharmacological research Physiological aspects Pools Prevention Protein Binding Proteins Public health Recombinant Fusion Proteins Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Recombinant Fusion Proteins - metabolism Research and Analysis Methods Servers T cells Tropical diseases Tumor Necrosis Factor-alpha Tumor Necrosis Factor-alpha - analysis Tumor necrosis factor-α Vaccines Vector-borne diseases Volunteers Young Adult γ-Interferon |
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| Title | Design of multi-epitope peptides containing HLA class-I and class-II-restricted epitopes derived from immunogenic Leishmania proteins, and evaluation of CD4+ and CD8+ T cell responses induced in cured cutaneous leishmaniasis subjects |
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