Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires

• Published in: Journal of nanobiotechnology Vol. 18; no. 1; pp. 1 - 12
• Main Authors: Takemura, Kenshin, Satoh, Jun, Boonyakida, Jirayu, Park, Sungjo, Chowdhury, Ankan Dutta, Park, Enoch Y.
• Format: Journal Article
• Language: English
• Published: London BioMed Central 27.10.2020; BioMed Central Ltd; BMC
• Subjects: Antibodies; Antigens; Aquaculture; Biotechnology; Chemistry; Chemistry and Materials Science; Climate change; Conducting polymers; Damage prevention; Deoxyribonucleic acid; Design and construction; Diagnosis; Diagnostic software; Diagnostic systems; Disposable electrode; DNA; Electrochemical analysis; Electrochemical instruments; Electrochemical virus detection; Electrochemistry; Electrodes; Fourier transforms; Global warming; Globalization; Gold; Gold nanoparticle; Graphene; Graphene quantum dots; Hepatitis; Infections; Infectious diseases; Laboratories; Molecular Medicine; Mortality; Nanocomposites; Nanomaterials; Nanoparticles; Nanotechnology; Nanowires; Nitrogen; Polyaniline; Polyanilines; Polymerization; Quantum dots; Rubber; Rubber medical supplies; Scanning electron microscopy; Selectivity; Sensitivity analysis; Silicone rubber; Silicones; Stability; Sulfur; Sulfur compounds; Transmission electron microscopy; Viral infections; Virus diseases; Viruses; White spot syndrome; White spot syndrome virus; Japan; United Kingdom > UK; United States > US; Electrochemical virus detection; White spot syndrome virus; Disposable electrode; Polyaniline; Gold nanoparticle; Graphene quantum dots
• ISSN: 1477-3155; 1477-3155
• DOI: 10.1186/s12951-020-00712-4

Background With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a disposable electrode with high-sensitivity and accuracy to evaluate its performances for several target viruses. Results Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-co-doped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of concentration from 1.45 × 10 2 to 1.45 × 10 5 DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml. Conclusion The engineered sensor electrode can retain the detection activity up to 5 weeks, to confirm its long-term stability, required for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing as a potential diagnostic tool to monitor WSSV in the aquaculture industry.