Yuzu (Citrus junos Tanaka) Peel Attenuates Dextran Sulfate Sodium-induced Murine Experimental Colitis

Ulcerative colitis is a well-known inflammatory bowel disease. Although there are drugs that are effective against this disease, the prevention and attenuation of ulcerative colitis by food rich in functional ingredients without side effects is desired because some drugs have side effects. In this s...

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Published inJournal of Oleo Science Vol. 67; no. 3; pp. 335 - 344
Main Authors Fujita, Yasuko, Ohnishi, Shigehiko, Shichiri, Mototada, Yasunaga, Mayu, Ishida, Noriko, Ishioka, Mika, Suzuki, Shingo, Umeno, Aya, Yoshida, Yasukazu, Sato, Akihiko, Nakajima, Yoshihiro, Abe, Hiroko
Format Journal Article
LanguageEnglish
Published Japan Japan Oil Chemists' Society 01.03.2018
Japan Science and Technology Agency
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Online AccessGet full text
ISSN1345-8957
1347-3352
1347-3352
DOI10.5650/jos.ess17184

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Abstract Ulcerative colitis is a well-known inflammatory bowel disease. Although there are drugs that are effective against this disease, the prevention and attenuation of ulcerative colitis by food rich in functional ingredients without side effects is desired because some drugs have side effects. In this study, we investigated the effects of yuzu (Citrus junos Tanaka), a citrus fruit native to northeast Asia, on a mouse dextran sulfate sodium (DSS)-induced colitis model. Mice given drinking water containing DSS showed significant weight loss, colon shortening, diarrhea, and visible fecal blood. In contrast, mice fed a diet containing 5% yuzu peel for 14 d before receiving DSS showed significant attenuation of these phenotypes. To clarify the mechanism underlying the attenuation, we investigated the anti-inflammatory and antioxidant effects of yuzu peel. We found that yuzu peel extract suppressed tumor necrosis factor-α (TNF-α) production in lipopolysaccharide (LPS)-stimulated mice and murine macrophage cell line through suppression of nuclear factor-κB (NF-κB) activation. In addition, we confirmed that yuzu peel extract had a moderate antioxidant effect. These results suggest that yuzu peel attenuates the pathologies of DSS-induced colitis by coordinately suppressing inflammation and oxidative stress against lipids in vivo.
AbstractList Ulcerative colitis is a well-known inflammatory bowel disease. Although there are drugs that are effective against this disease, the prevention and attenuation of ulcerative colitis by food rich in functional ingredients without side effects is desired because some drugs have side effects. In this study, we investigated the effects of yuzu (Citrus junos Tanaka), a citrus fruit native to northeast Asia, on a mouse dextran sulfate sodium (DSS)-induced colitis model. Mice given drinking water containing DSS showed significant weight loss, colon shortening, diarrhea, and visible fecal blood. In contrast, mice fed a diet containing 5% yuzu peel for 14 d before receiving DSS showed significant attenuation of these phenotypes. To clarify the mechanism underlying the attenuation, we investigated the anti-inflammatory and antioxidant effects of yuzu peel. We found that yuzu peel extract suppressed tumor necrosis factor-α (TNF-α) production in lipopolysaccharide (LPS)-stimulated mice and murine macrophage cell line through suppression of nuclear factor-κB (NF-κB) activation. In addition, we confirmed that yuzu peel extract had a moderate antioxidant effect. These results suggest that yuzu peel attenuates the pathologies of DSS-induced colitis by coordinately suppressing inflammation and oxidative stress against lipids in vivo.
Abstract: Ulcerative colitis is a well-known inflammatory bowel disease. Although there are drugs that are effective against this disease, the prevention and attenuation of ulcerative colitis by food rich in functional ingredients without side effects is desired because some drugs have side effects. In this study, we investigated the effects of yuzu (Citrus junos Tanaka), a citrus fruit native to northeast Asia, on a mouse dextran sulfate sodium (DSS)-induced colitis model. Mice given drinking water containing DSS showed significant weight loss, colon shortening, diarrhea, and visible fecal blood. In contrast, mice fed a diet containing 5% yuzu peel for 14 d before receiving DSS showed significant attenuation of these phenotypes. To clarify the mechanism underlying the attenuation, we investigated the anti-inflammatory and antioxidant effects of yuzu peel. We found that yuzu peel extract suppressed tumor necrosis factor-α (TNF-α) production in lipopolysaccharide (LPS)-stimulated mice and murine macrophage cell line through suppression of nuclear factor-кB (NF-кB) activation. In addition, we confirmed that yuzu peel extract had a moderate antioxidant effect. These results suggest that yuzu peel attenuates the pathologies of DSS-induced colitis by coordinately suppressing inflammation and oxidative stress against lipids in vivo.
Author Fujita, Yasuko
Ishioka, Mika
Suzuki, Shingo
Sato, Akihiko
Ohnishi, Shigehiko
Umeno, Aya
Abe, Hiroko
Yasunaga, Mayu
Nakajima, Yoshihiro
Yoshida, Yasukazu
Ishida, Noriko
Shichiri, Mototada
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  fullname: Fujita, Yasuko
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  fullname: Yasunaga, Mayu
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antioxidant against lipids
inflammation
oxidative stress
citrus flavonoid
yuzu
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References_xml – reference: 13) Sawamura, M.; Wu, Y.; Fujiwara, C.; Urushibata, M. Inhibitory effect of yuzu essential oil on the formation of N-nitrosodimethylamine in vegetables. J. Agric. Food Chem. 53, 4281-4287 (2005).
– reference: 10) Miao, X.P.; Sun, X.N.; Cui, L.J.; Cao, Q.F.; Zhuang, G.F.; Deng, T.Z.; Zhang, D.Y. Suppressive effect of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on inflammation by regulation of NF- kappa B pathway and interleukin-17 in mice with dextran sulphatesodium-induced ulcerative colitis. Asian Pac. J. Trop. Med. 8, 147-152 (2015).
– reference: 8) Petronilho, F.; Michels, M.; Danielski, L.G.; Goldim, M.P.; Florentino, D.; Vieira, A.; Mendonca, M.G.; Tournier, M.; Piacentini, B.; Giustina, A.D.; Leffa, D.D.; Pereira, G.W.; Pereira, V.D.; Rocha, J.B. Diphenyl diselenide attenuates oxidative stress and inflammatory parameters in ulcerative colitis: A comparison with ebselen. Pathol. Res. Pract. 212, 755-760 (2016).
– reference: 7) Babbs, C.F. Oxygen radicals in ulcerative colitis. Free Radic. Biol. Med. 13, 169-181 (1992).
– reference: 29) Tan, Z.; Cheng, J.; Liu, Q.; Zhou, L.; Kenny, J.; Wang, T.; Lin, X.; Yuan, J.; Quinn, J.M.; Tickner, J.; Hong, G.; Qin, A.; Zhao, J.; Xu, J. Neohesperidin suppresses osteoclast differentiation, bone resorption and ovariecto-mised-induced osteoporosis in mice. Mol. Cell Endocrinol. 439, 369-378 (2017).
– reference: 9) Reddy, J.G.; Loftus, E.V.Jr. Safety of infliximab and other biologic agents in the inflammatory bowel diseases. Gastroenterol. Clin. North Am. 35, 837-855 (2006).
– reference: 15) Kuraya, E.; Nakada, S.; Touyama, A.; Itoh, S. Improving the antioxidant functionality of Citrus junos Tanaka (yuzu) fruit juice by underwater shockwave pretreatment. Food Chem. 216, 123-129 (2017).
– reference: 28) Kanno, S.; Shouji, A.; Tomizawa, A.; Hiura, T.; Osanai, Y.; Ujibe, M.; Obara, Y.; Nakahata, N.; Ishikawa, M. Inhibitory effect of naringin on lipopolysaccharide (LPS) - induced endotoxin shock in mice and nitric oxide production in RAW 264.7 macrophages. Life Sci. 78, 673-681 (2006).
– reference: 30) Freeman, B.L.; Eggett, D.L.; Parker, T.L. Synergistic and antagonistic interactions of phenolic compounds found in navel oranges. J. Food Sci. 75, C570-576 (2010).
– reference: 23) Tsuboi, Y.; Abe, H.; Nakagawa, R.; Oomizu, S.; Watanabe, K.; Nishi, N.; Nakamura, T.; Yamauchi, A.; Hirashima, M. Galectin-9 protects mice from the Shwartzman reaction by attracting prostaglandin E2-producing polymorphonuclear leukocytes. Clin. Immunol. 124, 221-233 (2007).
– reference: 17) Kim, S.H.; Hur, H.J.; Yang, H.J.; Kim, H.J.; Kim, M.J.; Park, J.H.; Sung, M.J.; Kim, M.S.; Kwon, D.Y.; Hwang, J.T. Citrus junos Tanaka peel extract exerts antidiabetic effects via AMPK and PPAR-gamma both in vitro and in vivo in mice fed a high-fat diet. Evid. Based Complement. Alternat. Med. 2013, 921012 (2013).
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Snippet Ulcerative colitis is a well-known inflammatory bowel disease. Although there are drugs that are effective against this disease, the prevention and attenuation...
Abstract: Ulcerative colitis is a well-known inflammatory bowel disease. Although there are drugs that are effective against this disease, the prevention and...
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SubjectTerms Administration, Oral
Animals
Anti-Inflammatory Agents - pharmacology
antioxidant against lipids
Antioxidants
Antioxidants - pharmacology
Attenuation
Cell Line
Citrus - chemistry
citrus flavonoid
Citrus fruits
Colitis, Ulcerative - chemically induced
Colitis, Ulcerative - drug therapy
Colitis, Ulcerative - prevention & control
Colon
Dextran Sulfate - adverse effects
Disease Models, Animal
Drinking water
Drugs
experimental colitis
inflammation
Inflammatory bowel disease
Lipids
Macrophages - metabolism
Male
Mice
Mice, Inbred BALB C
NF-kappa B - metabolism
Oxidative stress
Oxidative Stress - drug effects
Phytotherapy
Plant Extracts - administration & dosage
Plant Extracts - isolation & purification
Side effects
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-TNF
Weight loss
yuzu
Title Yuzu (Citrus junos Tanaka) Peel Attenuates Dextran Sulfate Sodium-induced Murine Experimental Colitis
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