Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics

The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is unique in that efficient plant cell wall degradation relies on the cooperation between microorganisms that produce fibrolytic enzymes and the host anim...

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Published inFEMS microbiology reviews Vol. 27; no. 5; pp. 663 - 693
Main Authors Krause, Denis O, Denman, Stuart E, Mackie, Roderick I, Morrison, Mark, Rae, Ann L, Attwood, Graeme T, McSweeney, Christopher S
Format Journal Article
LanguageEnglish
Published Oxford, UK Elsevier B.V 01.12.2003
Blackwell Publishing Ltd
Blackwell
Oxford University Press
Subjects
Online AccessGet full text
ISSN0168-6445
1574-6976
1574-6976
DOI10.1016/S0168-6445(03)00072-X

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Abstract The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is unique in that efficient plant cell wall degradation relies on the cooperation between microorganisms that produce fibrolytic enzymes and the host animal that provides an anaerobic fermentation chamber. Increasing the efficiency with which the rumen microbiota degrades fiber has been the subject of extensive research for at least the last 100 years. Fiber digestion in the rumen is not optimal, as is supported by the fact that fiber recovered from feces is fermentable. This view is confirmed by the knowledge that mechanical and chemical pretreatments improve fiber degradation, as well as more recent research, which has demonstrated increased fiber digestion by rumen microorganisms when plant lignin composition is modified by genetic manipulation. Rumen microbiologists have sought to improve fiber digestion by genetic and ecological manipulation of rumen fermentation. This has been difficult and a number of constraints have limited progress, including: (a) a lack of reliable transformation systems for major fibrolytic rumen bacteria, (b) a poor understanding of ecological factors that govern persistence of fibrolytic bacteria and fungi in the rumen, (c) a poor understanding of which glycolyl hydrolases need to be manipulated, and (d) a lack of knowledge of the functional genomic framework within which fiber degradation operates. In this review the major fibrolytic organisms are briefly discussed. A more extensive discussion of the enzymes involved in fiber degradation is included. We also discuss the use of plant genetic manipulation, application of free-living lignolytic fungi and the use of exogenous enzymes. Lastly, we will discuss how newer technologies such as genomic and metagenomic approaches can be used to improve our knowledge of the functional genomic framework of plant cell wall degradation in the rumen.
AbstractList Abstract The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is unique in that efficient plant cell wall degradation relies on the cooperation between microorganisms that produce fibrolytic enzymes and the host animal that provides an anaerobic fermentation chamber. Increasing the efficiency with which the rumen microbiota degrades fiber has been the subject of extensive research for at least the last 100 years. Fiber digestion in the rumen is not optimal, as is supported by the fact that fiber recovered from feces is fermentable. This view is confirmed by the knowledge that mechanical and chemical pretreatments improve fiber degradation, as well as more recent research, which has demonstrated increased fiber digestion by rumen microorganisms when plant lignin composition is modified by genetic manipulation. Rumen microbiologists have sought to improve fiber digestion by genetic and ecological manipulation of rumen fermentation. This has been difficult and a number of constraints have limited progress, including: (a) a lack of reliable transformation systems for major fibrolytic rumen bacteria, (b) a poor understanding of ecological factors that govern persistence of fibrolytic bacteria and fungi in the rumen, (c) a poor understanding of which glycolyl hydrolases need to be manipulated, and (d) a lack of knowledge of the functional genomic framework within which fiber degradation operates. In this review the major fibrolytic organisms are briefly discussed. A more extensive discussion of the enzymes involved in fiber degradation is included. We also discuss the use of plant genetic manipulation, application of free-living lignolytic fungi and the use of exogenous enzymes. Lastly, we will discuss how newer technologies such as genomic and metagenomic approaches can be used to improve our knowledge of the functional genomic framework of plant cell wall degradation in the rumen.
The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is unique in that efficient plant cell wall degradation relies on the cooperation between microorganisms that produce fibrolytic enzymes and the host animal that provides an anaerobic fermentation chamber. Increasing the efficiency with which the rumen microbiota degrades fiber has been the subject of extensive research for at least the last 100 years. Fiber digestion in the rumen is not optimal, as is supported by the fact that fiber recovered from feces is fermentable. This view is confirmed by the knowledge that mechanical and chemical pretreatments improve fiber degradation, as well as more recent research, which has demonstrated increased fiber digestion by rumen microorganisms when plant lignin composition is modified by genetic manipulation. Rumen microbiologists have sought to improve fiber digestion by genetic and ecological manipulation of rumen fermentation. This has been difficult and a number of constraints have limited progress, including: (a) a lack of reliable transformation systems for major fibrolytic rumen bacteria, (b) a poor understanding of ecological factors that govern persistence of fibrolytic bacteria and fungi in the rumen, (c) a poor understanding of which glycolyl hydrolases need to be manipulated, and (d) a lack of knowledge of the functional genomic framework within which fiber degradation operates. In this review the major fibrolytic organisms are briefly discussed. A more extensive discussion of the enzymes involved in fiber degradation is included. We also discuss the use of plant genetic manipulation, application of free-living lignolytic fungi and the use of exogenous enzymes. Lastly, we will discuss how newer technologies such as genomic and metagenomic approaches can be used to improve our knowledge of the functional genomic framework of plant cell wall degradation in the rumen.
The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is unique in that efficient plant cell wall degradation relies on the cooperation between microorganisms that produce fibrolytic enzymes and the host animal that provides an anaerobic fermentation chamber. Increasing the efficiency with which the rumen microbiota degrades fiber has been the subject of extensive research for at least the last 100 years. Fiber digestion in the rumen is not optimal, as is supported by the fact that fiber recovered from feces is fermentable. This view is confirmed by the knowledge that mechanical and chemical pretreatments improve fiber degradation, as well as more recent research, which has demonstrated increased fiber digestion by rumen microorganisms when plant lignin composition is modified by genetic manipulation. Rumen microbiologists have sought to improve fiber digestion by genetic and ecological manipulation of rumen fermentation. This has been difficult and a number of constraints have limited progress, including: (a) a lack of reliable transformation systems for major fibrolytic rumen bacteria, (b) a poor understanding of ecological factors that govern persistence of fibrolytic bacteria and fungi in the rumen, (c) a poor understanding of which glycolyl hydrolases need to be manipulated, and (d) a lack of knowledge of the functional genomic framework within which fiber degradation operates. In this review the major fibrolytic organisms are briefly discussed. A more extensive discussion of the enzymes involved in fiber degradation is included. We also discuss the use of plant genetic manipulation, application of free-living lignolytic fungi and the use of exogenous enzymes. Lastly, we will discuss how newer technologies such as genomic and metagenomic approaches can be used to improve our knowledge of the functional genomic framework of plant cell wall degradation in the rumen.The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is unique in that efficient plant cell wall degradation relies on the cooperation between microorganisms that produce fibrolytic enzymes and the host animal that provides an anaerobic fermentation chamber. Increasing the efficiency with which the rumen microbiota degrades fiber has been the subject of extensive research for at least the last 100 years. Fiber digestion in the rumen is not optimal, as is supported by the fact that fiber recovered from feces is fermentable. This view is confirmed by the knowledge that mechanical and chemical pretreatments improve fiber degradation, as well as more recent research, which has demonstrated increased fiber digestion by rumen microorganisms when plant lignin composition is modified by genetic manipulation. Rumen microbiologists have sought to improve fiber digestion by genetic and ecological manipulation of rumen fermentation. This has been difficult and a number of constraints have limited progress, including: (a) a lack of reliable transformation systems for major fibrolytic rumen bacteria, (b) a poor understanding of ecological factors that govern persistence of fibrolytic bacteria and fungi in the rumen, (c) a poor understanding of which glycolyl hydrolases need to be manipulated, and (d) a lack of knowledge of the functional genomic framework within which fiber degradation operates. In this review the major fibrolytic organisms are briefly discussed. A more extensive discussion of the enzymes involved in fiber degradation is included. We also discuss the use of plant genetic manipulation, application of free-living lignolytic fungi and the use of exogenous enzymes. Lastly, we will discuss how newer technologies such as genomic and metagenomic approaches can be used to improve our knowledge of the functional genomic framework of plant cell wall degradation in the rumen.
Author Mackie, Roderick I
Krause, Denis O
Attwood, Graeme T
Denman, Stuart E
Rae, Ann L
Morrison, Mark
McSweeney, Christopher S
Author_xml – sequence: 1
  givenname: Denis O
  surname: Krause
  fullname: Krause, Denis O
  email: denis_krause@umanitoba.ca
  organization: CSIRO Australia, Queensland Bioscience Precinct, St. Lucia, Qld 4067, Australia
– sequence: 2
  givenname: Stuart E
  surname: Denman
  fullname: Denman, Stuart E
  organization: CSIRO Australia, Queensland Bioscience Precinct, St. Lucia, Qld 4067, Australia
– sequence: 3
  givenname: Roderick I
  surname: Mackie
  fullname: Mackie, Roderick I
  organization: Department of Animal Science, University of Illinois, Champaign-Urbana, IL 61801, USA
– sequence: 4
  givenname: Mark
  surname: Morrison
  fullname: Morrison, Mark
  organization: The Ohio State University, Department of Animal Sciences, Columbus, OH 43210, USA
– sequence: 5
  givenname: Ann L
  surname: Rae
  fullname: Rae, Ann L
  organization: CSIRO Australia, Queensland Bioscience Precinct, St. Lucia, Qld 4067, Australia
– sequence: 6
  givenname: Graeme T
  surname: Attwood
  fullname: Attwood, Graeme T
  organization: AgResearch, Palmerston North, New Zealand
– sequence: 7
  givenname: Christopher S
  surname: McSweeney
  fullname: McSweeney, Christopher S
  organization: CSIRO Australia, Queensland Bioscience Precinct, St. Lucia, Qld 4067, Australia
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15304255$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/14638418$$D View this record in MEDLINE/PubMed
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ID FETCH-LOGICAL-c6543-64f00a668eecbdfd661cfa48af926fedda72d84b84b3a15e5246772ca7b83e93
IEDL.DBID DR2
ISSN 0168-6445
1574-6976
IngestDate Thu Oct 16 05:42:32 EDT 2025
Thu Sep 04 19:17:43 EDT 2025
Tue Oct 07 08:25:09 EDT 2025
Tue Oct 07 05:59:02 EDT 2025
Wed Feb 19 02:42:31 EST 2025
Mon Jul 21 09:14:42 EDT 2025
Thu Apr 24 23:05:55 EDT 2025
Wed Oct 01 02:18:47 EDT 2025
Wed Jan 22 16:36:15 EST 2025
Wed Sep 11 04:52:20 EDT 2024
Wed Oct 15 12:53:27 EDT 2025
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 5
Keywords Rumen
Plant cell wall
Functional genome
Metagenome
Diversity
Lignin
Enzyme
Genomics
Microflora
Review
Fermentation
Cell wall
Fungi
Bacteria
Genetics
Hydrolases
Feces
Thallophyta
Language English
License CC BY 4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c6543-64f00a668eecbdfd661cfa48af926fedda72d84b84b3a15e5246772ca7b83e93
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ObjectType-Article-2
ObjectType-Feature-1
content type line 23
ObjectType-Review-3
OpenAccessLink https://proxy.k.utb.cz/login?url=https://academic.oup.com/femsre/article-pdf/27/5/663/18145571/27-5-663.pdf
PMID 14638418
PQID 2476569518
PQPubID 986349
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PublicationTitle FEMS microbiology reviews
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PublicationYear 2003
Publisher Elsevier B.V
Blackwell Publishing Ltd
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Snippet The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is unique in...
Abstract The degradation of plant cell walls by ruminants is of major economic importance in the developed as well as developing world. Rumen fermentation is...
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StartPage 663
SubjectTerms Animals
Bacteria
Bacteriology
Biodegradation
Biological and medical sciences
Cell walls
Degradation
Dietary Fiber - metabolism
Dietary Fiber - microbiology
Digestion
Diversity
Ecology
Economic conditions
Economic importance
Enzymes
Fermentation
Functional genome
Fundamental and applied biological sciences. Psychology
Fungi
Genetic transformation
Genetics
Genomics
glycolyl hydrolase
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Lignin
Metagenome
Metagenomics
Microbiology
Microbiota
Microorganisms
Miscellaneous
Mycology
Plant cell wall
Rumen
Rumen - metabolism
Rumen - microbiology
Rumen microorganisms
Ruminants - microbiology
Virology
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Title Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics
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https://onlinelibrary.wiley.com/doi/abs/10.1016%2FS0168-6445%2803%2900072-X
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