重离子束辐照选育高产植物乳酸菌

采用重离子束~（12）C~（6＋）对乳酸菌出发菌株JTL进行辐照选育。确定吸收剂量300 Gy时最优,在该吸收剂量下,出发菌种的正突变率和致死率均达到最高,分别为34.4%、80%。通过酸斑法初筛和发酵复筛法,选育出高产酸的3株突变菌株（JTL2、JTL3、JTL5）,其发酵液中乳酸含量比出发菌株JTL提高了41.29%~49.64%。同时,经过传代实验证实,3株菌株具有良好的遗传稳定性。研究表明,利用重离子束~（12）C~（6＋）辐照处理乳酸菌可以得到高产酸的突变菌株,诱变效果显著。...

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Bibliographic Details
Published in辐射研究与辐射工艺学报 Vol. 35; no. 1; pp. 50 - 56
Main Author 王雨辰 王曙阳 董妙音 许富强 胡伟 陈积红 李文建 韩舜愈 刘敬
Format Journal Article
LanguageChinese
Published 中国科学院近代物理研究所生物物理室 兰州730000%中国科学院近代物理研究所生物物理室 兰州730000%中国科学院近代物理研究所生物物理室 兰州730000 2017
甘肃省辐照诱变育种工程实验室 兰州730000%甘肃农业大学食品科学与工程学院 兰州730070
甘肃农业大学食品科学与工程学院 兰州730070
Subjects
乳酸菌
诱变育种
辐照
重离子束
Lactobacillus plantarum
诱变育种
Heavy ion beam
重离子束
辐照
Irradiation
乳酸菌
Mutation breeding
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ISSN1000-3436
DOI10.11889/j.1000-3436.2017.rrj.35.010401

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Summary:采用重离子束~（12）C~（6＋）对乳酸菌出发菌株JTL进行辐照选育。确定吸收剂量300 Gy时最优,在该吸收剂量下,出发菌种的正突变率和致死率均达到最高,分别为34.4%、80%。通过酸斑法初筛和发酵复筛法,选育出高产酸的3株突变菌株（JTL2、JTL3、JTL5）,其发酵液中乳酸含量比出发菌株JTL提高了41.29%~49.64%。同时,经过传代实验证实,3株菌株具有良好的遗传稳定性。研究表明,利用重离子束~（12）C~（6＋）辐照处理乳酸菌可以得到高产酸的突变菌株,诱变效果显著。
Bibliography:12C6＋ ion beam was used to irradiate strains of Lactobacillus plantarum JTL, and the results showed that the strain could get the highest positive mutation rate and lethality with 34.4% and 80% at the optimal absorbed dose of 300 Gy. The strains which could produce higher lactic acid were screened by selective MRS mediums and fermentation experiments. Finally, three mutations JTL2, JTL3 and JTL5 were obtained through the two screening steps of selecting plate and fermentation, and the contents of lactic acid in this three fermentation broth were greater than the original strain by 41.29% to 49.64%. The pass-generation test indicated that the heredity character of these mutations were stable. It is demonstrated that the lactic acids produced by Lactobacillus plantarum JTL were remarkably improved, and heavy ion beam is an efficient radiation resource for microorganism breeding.
31-1258/TL
Lactobacillus plantarum, Heavy ion beam, Irradiation, Mutation breeding
WANG Yuchen1,2 WANG Shuyang1,2 DONG Miaoyin2 XU Fuqian
ISSN:1000-3436
DOI:10.11889/j.1000-3436.2017.rrj.35.010401