The Redox Potential Measurements for Heme Moieties in Variants of D-Fructose Dehydrogenase Based on Mediator-assisted Potentiometric Titration
The effect of mutation on the redox potentials (E°′) of the heme moieties in the variants of d -fructose dehydrogenase (FDH) was investigated by mediated spectroelectrochemical titrations. The replacement of the axial ligand of heme from methionine to glutamine changes the E°′ value more negatively...
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| Published in | Denki kagaku oyobi kōgyō butsuri kagaku Vol. 89; no. 4; pp. 337 - 339 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Tokyo
The Electrochemical Society of Japan
05.07.2021
Japan Science and Technology Agency |
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| Online Access | Get full text |
| ISSN | 1344-3542 2186-2451 2186-2451 |
| DOI | 10.5796/electrochemistry.21-00044 |
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| Abstract | The effect of mutation on the redox potentials (E°′) of the heme moieties in the variants of d -fructose dehydrogenase (FDH) was investigated by mediated spectroelectrochemical titrations. The replacement of the axial ligand of heme from methionine to glutamine changes the E°′ value more negatively than that of the corresponding heme moiety in the recombinant (native) FDH (rFDH). The determined E°′ values of non-targeted heme moieties in the variants were also shifted in a negative direction from that in rFDH. Thus, enzyme modification changes E°′ of the heme moieties in unmodified protein regions. |
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| AbstractList | The effect of mutation on the redox potentials (E°′) of the heme moieties in the variants of d-fructose dehydrogenase (FDH) was investigated by mediated spectroelectrochemical titrations. The replacement of the axial ligand of heme from methionine to glutamine changes the E°′ value more negatively than that of the corresponding heme moiety in the recombinant (native) FDH (rFDH). The determined E°′ values of non-targeted heme moieties in the variants were also shifted in a negative direction from that in rFDH. Thus, enzyme modification changes E°′ of the heme moieties in unmodified protein regions. |
| ArticleNumber | 21-00044 |
| Author | KITAZUMI, Yuki SOWA, Keisei SHIRAI, Osamu SUZUKI, Yohei |
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| SubjectTerms | D-Fructose Dehydrogenase Dehydrogenase Dehydrogenases Fructose Glutamine Heme Heme Moieties Methionine Mutation Mutations Potentiometric Titration Redox potential Titration |
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| Title | The Redox Potential Measurements for Heme Moieties in Variants of D-Fructose Dehydrogenase Based on Mediator-assisted Potentiometric Titration |
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