实时荧光PCR法检测鼠伤寒沙门氏菌

目的 建立实时荧光PCR法检测鼠伤寒沙门氏菌的方法.方法 基于鼠伤寒沙门氏菌Ⅱ型限制酶基因,设计引物及Taqman探针,利用实时荧光PCR进行特异性、灵敏性及模拟样品的检测实验.结果 特异性探针可从25种血清型沙门氏菌(共49株)及11株阴性对照菌株中检测出全部的11株鼠伤寒沙门氏菌.以鼠伤寒沙门氏菌梯度稀释菌液DNA为模板进行实时荧光PCR实验,菌株模板浓度与Ct值呈良好线性关系,线性系数(R2)为0.998,扩增效率90％,最低检测浓度300 cfu/mL.对已接种鼠伤寒沙门氏菌的4种模拟样品同时进行实时荧光PCR检测和传统方法鉴定,两者结果一致.结论 此方法特异、灵敏、准确,适于食品中鼠...

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Published in食品安全质量检测学报 Vol. 3; no. 4; pp. 300 - 305
Main Author 荣策;赵彤彤;许龙岩;刘宇;那晗;曹际娟
Format Journal Article
LanguageChinese
Published 大连工业大学生物工程学院,大连,116034%辽宁出入境检验检疫局,大连,116001%广东出入境检验检疫局,广州,510623 2012
Subjects
实时荧光PCR法;鼠伤寒沙门氏菌;Taqman探针;沙门氏菌属
实时荧光PCR法
Taqman探针
沙门氏菌属
鼠伤寒沙门氏菌
Online AccessGet full text
ISSN2095-0381

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Abstract 目的 建立实时荧光PCR法检测鼠伤寒沙门氏菌的方法.方法 基于鼠伤寒沙门氏菌Ⅱ型限制酶基因,设计引物及Taqman探针,利用实时荧光PCR进行特异性、灵敏性及模拟样品的检测实验.结果 特异性探针可从25种血清型沙门氏菌(共49株)及11株阴性对照菌株中检测出全部的11株鼠伤寒沙门氏菌.以鼠伤寒沙门氏菌梯度稀释菌液DNA为模板进行实时荧光PCR实验,菌株模板浓度与Ct值呈良好线性关系,线性系数(R2)为0.998,扩增效率90％,最低检测浓度300 cfu/mL.对已接种鼠伤寒沙门氏菌的4种模拟样品同时进行实时荧光PCR检测和传统方法鉴定,两者结果一致.结论 此方法特异、灵敏、准确,适于食品中鼠伤寒沙门氏菌的检测.
AbstractList 目的 建立实时荧光PCR法检测鼠伤寒沙门氏菌的方法.方法 基于鼠伤寒沙门氏菌Ⅱ型限制酶基因,设计引物及Taqman探针,利用实时荧光PCR进行特异性、灵敏性及模拟样品的检测实验.结果 特异性探针可从25种血清型沙门氏菌(共49株)及11株阴性对照菌株中检测出全部的11株鼠伤寒沙门氏菌.以鼠伤寒沙门氏菌梯度稀释菌液DNA为模板进行实时荧光PCR实验,菌株模板浓度与Ct值呈良好线性关系,线性系数(R2)为0.998,扩增效率90％,最低检测浓度300 cfu/mL.对已接种鼠伤寒沙门氏菌的4种模拟样品同时进行实时荧光PCR检测和传统方法鉴定,两者结果一致.结论 此方法特异、灵敏、准确,适于食品中鼠伤寒沙门氏菌的检测.
Author 荣策;赵彤彤;许龙岩;刘宇;那晗;曹际娟
AuthorAffiliation 大连工业大学生物工程学院,大连,116034;辽宁出入境检验检疫局,大连,116001;广东出入境检验检疫局,广州,510623
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ZHAO Tong-Tong
XU Long-Yan
CAO Ji-Juan
RONG Ce
NA Han
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Taqman探针
沙门氏菌属
鼠伤寒沙门氏菌
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Title 实时荧光PCR法检测鼠伤寒沙门氏菌
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