Genomic variation and DNA repair associated with soybean transgenesis: a comparison to cultivars and mutagenized plants

Background The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this stu...

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Published inBMC biotechnology Vol. 16; no. 1; p. 41
Main Authors Anderson, Justin E., Michno, Jean-Michel, Kono, Thomas J. Y., Stec, Adrian O., Campbell, Benjamin W., Curtin, Shaun J., Stupar, Robert M.
Format Journal Article
LanguageEnglish
Published London BioMed Central 12.05.2016
BioMed Central Ltd
Subjects
Agrobacterium
Applied Microbiology
Biochemical Engineering
Biomedical Engineering/Biotechnology
Biotechnology
Chemistry
Chemistry and Materials Science
crops
cultivars
DNA Damage - genetics
DNA repair
DNA Repair - genetics
Gene Expression Regulation, Plant - genetics
gene transfer
Genetic aspects
Genetic Engineering
Genetic Engineering - methods
Genetic research
Genetic transformation
genetic variation
Genetic Variation - genetics
Genome, Plant - genetics
genomics
germplasm
Glycine max - genetics
Health aspects
Identification and classification
loci
mutagenesis
mutants
Mutation - genetics
neutrons
phenotype
Plant biotechnology
Plant Breeding/Biotechnology
Plants, Genetically Modified - genetics
Research Article
single nucleotide polymorphism
Single nucleotide polymorphisms
Soybean
soybeans
transgenes
transgenesis
transgenic plants
Biotechnology
Genetic engineering
Soybean
Somaclonal variation
Structural variation
Transgenic crops
Online AccessGet full text
ISSN1472-6750
1472-6750
DOI10.1186/s12896-016-0271-z

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Abstract Background The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods. Results On average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms. Conclusions Overall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods.
AbstractList The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods. On average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms. Overall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods.
Background The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods. Results On average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms. Conclusions Overall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods.
The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods.BACKGROUNDThe safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods.On average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms.RESULTSOn average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms.Overall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods.CONCLUSIONSOverall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods.
BACKGROUND: The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods. RESULTS: On average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms. CONCLUSIONS: Overall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods.
Background The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods. Results On average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms. Conclusions Overall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods. Keywords: Somaclonal variation, Structural variation, Genetic engineering, Biotechnology, Transgenic crops, Soybean
The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular variation induced by gene transfer technologies will provide a scientific-based means to rationally address such concerns. In this study, genomic structural variation (e.g. large deletions and duplications) and single nucleotide polymorphism rates were assessed among a sample of soybean cultivars, fast neutron-derived mutants, and five genetically transformed plants developed through Agrobacterium based transformation methods. On average, the number of genes affected by structural variations in transgenic plants was one order of magnitude less than that of fast neutron mutants and two orders of magnitude less than the rates observed between cultivars. Structural variants in transgenic plants, while rare, occurred adjacent to the transgenes, and at unlinked loci on different chromosomes. DNA repair junctions at both transgenic and unlinked sites were consistent with sequence microhomology across breakpoints. The single nucleotide substitution rates were modest in both fast neutron and transformed plants, exhibiting fewer than 100 substitutions genome-wide, while inter-cultivar comparisons identified over one-million single nucleotide polymorphisms. Overall, these patterns provide a fresh perspective on the genomic variation associated with high-energy induced mutagenesis and genetically transformed plants. The genetic transformation process infrequently results in novel genetic variation and these rare events are analogous to genetic variants occurring spontaneously, already present in the existing germplasm, or induced through other types of mutagenesis. It remains unclear how broadly these results can be applied to other crops or transformation methods.
ArticleNumber 41
Audience Academic
Author Stupar, Robert M.
Stec, Adrian O.
Curtin, Shaun J.
Anderson, Justin E.
Kono, Thomas J. Y.
Michno, Jean-Michel
Campbell, Benjamin W.
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Cites_doi 10.1093/dnares/dst036
10.1080/00087114.1997.10797380
10.1371/journal.pone.0149515
10.1038/nrg3642
10.1104/pp.111.181206
10.3835/plantgenome2014.10.0070
10.1534/g3.115.022137
10.1104/pp.112.204271
10.1111/j.1365-313X.2010.04386.x
10.2135/cropsci1992.0011183X003200030058x
10.1101/gr.092759.109
10.1111/j.1467-7652.2004.00070.x
10.2135/cropsci2004.6930
10.1093/bioinformatics/btp324
10.1007/s00299-005-0048-7
10.1534/g3.114.011551
10.1007/s11248-015-9867-7
10.1016/j.cub.2011.07.002
10.1104/pp.110.170811
10.1007/s00299-014-1572-0
10.1007/s11248-014-9843-7
10.1186/s12864-015-1285-y
10.1038/ng.715
10.3835/plantgenome2012.10.0026
10.1104/pp.126.4.1527
10.1023/A:1006491613768
10.1534/genetics.114.170340
10.1023/A:1004124519479
10.1104/pp.111.172981
10.1007/s00299-011-1202-z
10.1093/pcp/pcu153
10.1111/j.1365-313X.2011.04492.x
10.1093/genetics/149.2.641
10.1038/ng.806
10.1104/pp.112.194605
10.1104/pp.110.166736
10.1007/s00122-013-2177-7
10.1093/bib/bbs017
10.1186/1471-2164-15-367
10.1093/pcp/pcr172
10.1023/A:1015685501358
10.1101/gr.131474.111
10.1023/A:1023929630687
10.2307/2441162
10.1038/nature08670
10.1038/nature06856
10.1371/journal.pone.0096879
10.1038/nmeth.1923
10.1534/g3.114.015255
10.1126/science.1180677
10.1021/jf073505i
10.1016/j.tig.2008.08.007
10.1038/nbt.3096
10.1016/j.molp.2016.03.009
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Issue 1
Keywords Biotechnology
Genetic engineering
Soybean
Somaclonal variation
Structural variation
Transgenic crops
Language English
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References F Sabot (271_CR14) 2011; 66
YT Bolon (271_CR1) 2014; 198
B Langmead (271_CR56) 2012; 9
R Ming (271_CR19) 2008; 452
DJ Heinz (271_CR6) 1971; 58
SM Jain (271_CR7) 2001; 118
A Zmienko (271_CR27) 2014; 127
J Schmutz (271_CR50) 2010; 463
S Ossowski (271_CR36) 2010; 327
GS Ladics (271_CR26) 2015; 24
T Kawakatsu (271_CR13) 2013; 20
271_CR38
CN Hancock (271_CR32) 2011; 157
H Thorvaldsdottir (271_CR53) 2013; 14
MM Paz (271_CR46) 2006; 25
BB Majhi (271_CR39) 2014; 33
WJ Haun (271_CR31) 2011; 155
JE Anderson (271_CR29) 2014; 4
SJ Curtin (271_CR47) 2015; 6
N Weber (271_CR42) 2012; 160
D Sims (271_CR25) 2014; 15
AK Neelakandan (271_CR2) 2012; 31
J Schnell (271_CR4) 2015; 24
271_CR24
KC Cheng (271_CR41) 2008; 56
JR Latham (271_CR3) 2006; 2006
JH Orf (271_CR44) 2004; 44
RL Bernard (271_CR49) 1988; 28
M McVey (271_CR37) 2008; 24
SK Guttikonda (271_CR22) 2016; 11
H Li (271_CR55) 2009; 25
SK Svitashev (271_CR16) 2002; 69
LK McHale (271_CR28) 2012; 159
EJ Belfield (271_CR35) 2012; 22
D Kovalic (271_CR23) 2012; 5
YT Bolon (271_CR43) 2011; 156
271_CR54
F D’Amato (271_CR5) 1997; 50
271_CR57
A Miyao (271_CR10) 2012; 53
C Jiang (271_CR8) 2011; 21
PM Olhoft (271_CR21) 2004; 2
BW Campbell (271_CR48) 2014; 5
MWM Muskens (271_CR18) 2000; 43
FE Tax (271_CR40) 2001; 126
M Krzywinski (271_CR58) 2009; 19
K Kashima (271_CR12) 2015; 16
A Forsbach (271_CR20) 2003; 52
SJ Curtin (271_CR45) 2011; 156
HM Lam (271_CR33) 2010; 42
T Singer (271_CR51) 2003; 236
JH Orf (271_CR30) 1992; 32
A Srivastava (271_CR52) 2014; 15
D Zhang (271_CR11) 2014; 9
Z Zhou (271_CR34) 2015; 33
P Nacry (271_CR15) 1998; 149
KA Clark (271_CR17) 2010; 64
M Endo (271_CR9) 2015; 56
References_xml – volume: 20
  start-page: 623
  issue: 6
  year: 2013
  ident: 271_CR13
  publication-title: DNA Res
  doi: 10.1093/dnares/dst036
– volume: 2006
  start-page: 25376
  issue: 2
  year: 2006
  ident: 271_CR3
  publication-title: J Biomed Biotechnol
– volume: 50
  start-page: 1
  issue: 1
  year: 1997
  ident: 271_CR5
  publication-title: Caryologia
  doi: 10.1080/00087114.1997.10797380
– volume: 11
  start-page: e0149515
  issue: 2
  year: 2016
  ident: 271_CR22
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0149515
– volume: 15
  start-page: 121
  issue: 2
  year: 2014
  ident: 271_CR25
  publication-title: Nat Rev Genet
  doi: 10.1038/nrg3642
– volume: 157
  start-page: 552
  issue: 2
  year: 2011
  ident: 271_CR32
  publication-title: Plant Physiol
  doi: 10.1104/pp.111.181206
– ident: 271_CR24
  doi: 10.3835/plantgenome2014.10.0070
– volume: 6
  start-page: 423
  issue: 2
  year: 2015
  ident: 271_CR47
  publication-title: G3 (Bethesda)
  doi: 10.1534/g3.115.022137
– volume: 160
  start-page: 1842
  issue: 4
  year: 2012
  ident: 271_CR42
  publication-title: Plant Physiol
  doi: 10.1104/pp.112.204271
– volume: 64
  start-page: 990
  issue: 6
  year: 2010
  ident: 271_CR17
  publication-title: Plant J
  doi: 10.1111/j.1365-313X.2010.04386.x
– volume: 32
  start-page: 830
  issue: 3
  year: 1992
  ident: 271_CR30
  publication-title: Crop Sci
  doi: 10.2135/cropsci1992.0011183X003200030058x
– volume: 19
  start-page: 1639
  issue: 9
  year: 2009
  ident: 271_CR58
  publication-title: Genome Res
  doi: 10.1101/gr.092759.109
– volume: 2
  start-page: 289
  issue: 4
  year: 2004
  ident: 271_CR21
  publication-title: Plant Biotechnol J
  doi: 10.1111/j.1467-7652.2004.00070.x
– volume: 44
  start-page: 693
  issue: 2
  year: 2004
  ident: 271_CR44
  publication-title: Crop Sci
  doi: 10.2135/cropsci2004.6930
– volume: 25
  start-page: 1754
  issue: 14
  year: 2009
  ident: 271_CR55
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btp324
– volume: 25
  start-page: 206
  issue: 3
  year: 2006
  ident: 271_CR46
  publication-title: Plant Cell Rep
  doi: 10.1007/s00299-005-0048-7
– volume: 4
  start-page: 1307
  issue: 7
  year: 2014
  ident: 271_CR29
  publication-title: G3 (Bethesda)
  doi: 10.1534/g3.114.011551
– volume: 24
  start-page: 587
  issue: 4
  year: 2015
  ident: 271_CR26
  publication-title: Transgenic Res
  doi: 10.1007/s11248-015-9867-7
– volume: 21
  start-page: 1385
  issue: 16
  year: 2011
  ident: 271_CR8
  publication-title: Curr Biol
  doi: 10.1016/j.cub.2011.07.002
– volume: 156
  start-page: 240
  issue: 1
  year: 2011
  ident: 271_CR43
  publication-title: Plant Physiol
  doi: 10.1104/pp.110.170811
– volume: 33
  start-page: 929
  issue: 6
  year: 2014
  ident: 271_CR39
  publication-title: Plant Cell Rep
  doi: 10.1007/s00299-014-1572-0
– volume: 28
  start-page: 1027
  issue: 6
  year: 1988
  ident: 271_CR49
  publication-title: Crop Sci
– volume: 24
  start-page: 1
  issue: 1
  year: 2015
  ident: 271_CR4
  publication-title: Transgenic Res
  doi: 10.1007/s11248-014-9843-7
– volume: 16
  start-page: 48
  year: 2015
  ident: 271_CR12
  publication-title: BMC Genomics
  doi: 10.1186/s12864-015-1285-y
– volume: 42
  start-page: 1053
  issue: 12
  year: 2010
  ident: 271_CR33
  publication-title: Nat Genet
  doi: 10.1038/ng.715
– volume: 5
  start-page: 149
  issue: 3
  year: 2012
  ident: 271_CR23
  publication-title: Plant Genome
  doi: 10.3835/plantgenome2012.10.0026
– volume: 126
  start-page: 1527
  issue: 4
  year: 2001
  ident: 271_CR40
  publication-title: Plant Physiol
  doi: 10.1104/pp.126.4.1527
– volume: 43
  start-page: 243
  issue: 2
  year: 2000
  ident: 271_CR18
  publication-title: Plant Mol Biol
  doi: 10.1023/A:1006491613768
– volume: 198
  start-page: 967
  issue: 3
  year: 2014
  ident: 271_CR1
  publication-title: Genetics
  doi: 10.1534/genetics.114.170340
– volume: 236
  start-page: 241
  year: 2003
  ident: 271_CR51
  publication-title: Methods Mol Biol
– volume: 118
  start-page: 153
  issue: 2
  year: 2001
  ident: 271_CR7
  publication-title: Euphytica
  doi: 10.1023/A:1004124519479
– volume: 156
  start-page: 466
  issue: 2
  year: 2011
  ident: 271_CR45
  publication-title: Plant Physiol
  doi: 10.1104/pp.111.172981
– volume: 31
  start-page: 597
  issue: 4
  year: 2012
  ident: 271_CR2
  publication-title: Plant Cell Rep
  doi: 10.1007/s00299-011-1202-z
– volume: 56
  start-page: 116
  issue: 1
  year: 2015
  ident: 271_CR9
  publication-title: Plant Cell Physiol
  doi: 10.1093/pcp/pcu153
– volume: 66
  start-page: 241
  issue: 2
  year: 2011
  ident: 271_CR14
  publication-title: Plant J
  doi: 10.1111/j.1365-313X.2011.04492.x
– volume: 149
  start-page: 641
  issue: 2
  year: 1998
  ident: 271_CR15
  publication-title: Genetics
  doi: 10.1093/genetics/149.2.641
– ident: 271_CR57
  doi: 10.1038/ng.806
– volume: 159
  start-page: 1295
  issue: 4
  year: 2012
  ident: 271_CR28
  publication-title: Plant Physiol
  doi: 10.1104/pp.112.194605
– volume: 155
  start-page: 645
  issue: 2
  year: 2011
  ident: 271_CR31
  publication-title: Plant Physiol
  doi: 10.1104/pp.110.166736
– volume: 127
  start-page: 1
  issue: 1
  year: 2014
  ident: 271_CR27
  publication-title: Theor Appl Genet
  doi: 10.1007/s00122-013-2177-7
– volume: 14
  start-page: 178
  issue: 2
  year: 2013
  ident: 271_CR53
  publication-title: Brief Bioinform
  doi: 10.1093/bib/bbs017
– volume: 15
  start-page: 367
  year: 2014
  ident: 271_CR52
  publication-title: BMC Genomics
  doi: 10.1186/1471-2164-15-367
– volume: 53
  start-page: 256
  issue: 1
  year: 2012
  ident: 271_CR10
  publication-title: Plant Cell Physiol
  doi: 10.1093/pcp/pcr172
– volume: 69
  start-page: 205
  issue: 3
  year: 2002
  ident: 271_CR16
  publication-title: Plant Cell Tissue Organ Cult
  doi: 10.1023/A:1015685501358
– volume: 22
  start-page: 1306
  issue: 7
  year: 2012
  ident: 271_CR35
  publication-title: Genome Res
  doi: 10.1101/gr.131474.111
– volume: 52
  start-page: 161
  issue: 1
  year: 2003
  ident: 271_CR20
  publication-title: Plant Mol Biol
  doi: 10.1023/A:1023929630687
– volume: 58
  start-page: 257
  issue: 3
  year: 1971
  ident: 271_CR6
  publication-title: Am J Bot
  doi: 10.2307/2441162
– volume: 463
  start-page: 178
  issue: 7278
  year: 2010
  ident: 271_CR50
  publication-title: Nature
  doi: 10.1038/nature08670
– volume: 452
  start-page: 991
  issue: 7190
  year: 2008
  ident: 271_CR19
  publication-title: Nature
  doi: 10.1038/nature06856
– volume: 9
  start-page: e96879
  issue: 5
  year: 2014
  ident: 271_CR11
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0096879
– volume: 9
  start-page: 357
  issue: 4
  year: 2012
  ident: 271_CR56
  publication-title: Nat Methods
  doi: 10.1038/nmeth.1923
– volume: 5
  start-page: 123
  issue: 1
  year: 2014
  ident: 271_CR48
  publication-title: G3 (Bethesda)
  doi: 10.1534/g3.114.015255
– volume: 327
  start-page: 92
  issue: 5961
  year: 2010
  ident: 271_CR36
  publication-title: Science
  doi: 10.1126/science.1180677
– volume: 56
  start-page: 3057
  issue: 9
  year: 2008
  ident: 271_CR41
  publication-title: J Agric Food Chem
  doi: 10.1021/jf073505i
– volume: 24
  start-page: 529
  issue: 11
  year: 2008
  ident: 271_CR37
  publication-title: Trends Genet
  doi: 10.1016/j.tig.2008.08.007
– volume: 33
  start-page: 408
  issue: 4
  year: 2015
  ident: 271_CR34
  publication-title: Nat Biotechnol
  doi: 10.1038/nbt.3096
– ident: 271_CR54
– ident: 271_CR38
  doi: 10.1016/j.molp.2016.03.009
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Snippet Background The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and...
The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and molecular...
Background The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and...
BACKGROUND: The safety of mutagenized and genetically transformed plants remains a subject of scrutiny. Data gathered and communicated on the phenotypic and...
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SubjectTerms Agrobacterium
Applied Microbiology
Biochemical Engineering
Biomedical Engineering/Biotechnology
Biotechnology
Chemistry
Chemistry and Materials Science
crops
cultivars
DNA Damage - genetics
DNA repair
DNA Repair - genetics
Gene Expression Regulation, Plant - genetics
gene transfer
Genetic aspects
Genetic Engineering
Genetic Engineering - methods
Genetic research
Genetic transformation
genetic variation
Genetic Variation - genetics
Genome, Plant - genetics
genomics
germplasm
Glycine max - genetics
Health aspects
Identification and classification
loci
mutagenesis
mutants
Mutation - genetics
neutrons
phenotype
Plant biotechnology
Plant Breeding/Biotechnology
Plants, Genetically Modified - genetics
Research Article
single nucleotide polymorphism
Single nucleotide polymorphisms
Soybean
soybeans
transgenes
transgenesis
transgenic plants
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Title Genomic variation and DNA repair associated with soybean transgenesis: a comparison to cultivars and mutagenized plants
URI https://link.springer.com/article/10.1186/s12896-016-0271-z
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