Parallel on-chip gene synthesis and application to optimization of protein expression - Quan, Jiayuan; Saaem, Ishtiaq; Tang, Nicholas; Ma, Siying; Negre, Nicolas; Gong, Hui; White, Kevin P; Tian, Jingdong (Nature biotechnology, 2011)
Keywords: 631/61/338/552; Agriculture; Algorithms; Animals; Antigens; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biosynthesis; Biotechnology; Cellular proteins; Codon - genetics; Deoxyribonucleic acid; DNA; Drosophila; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; E coli; Escherichia coli - genetics; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Genes; Genes, Synthetic; Genetic engineering; Genetic technics; Insects; Lac Operon - genetics; letter; Life Sciences; Methods. Procedures. Technologies; Nucleotide sequence; Oligonucleotide Array Sequence Analysis - methods; Physiological aspects; Protein Engineering - methods; Proteins; Proteomics - methods; Sequence Analysis, DNA; Synthetic digonucleotides and genes. Sequencing; Transcription Factors - genetics; United States; Amplification; Gene; Oligonucleotide; System on a chip; Synthetic biology; Gene expression; Chemical synthesis; Optimization; Protein