芽孢杆菌在肉鸡肠道内的分布及对肠道菌群、消化酶活性的影响

考察枯草芽孢杆菌的芽孢在AA肉鸡肠道内的分布和随粪便的排出规律,及对肠道内细菌群落结构变化和肠道内消化酶活性的影响.试验选取240羽1日龄AA肉鸡,随机分为2组,每组6个重复,采用相同的无抗生素基础日粮饲养3 d后,对照组和试验组分别一次性灌喂无菌水或枯草芽孢杆菌的芽孢（1.5×108CFU？mL-1）各0.5 mL;在不同时间采样进行计数和酶活性测定,同时通过聚合酶链反应-变性梯度凝胶电泳（polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE）技术分析此过程肉鸡粪便中细菌群落的变化.结果表明：灌喂...

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Published in浙江大学学报（农业与生命科学版） Vol. 37; no. 3; pp. 319 - 325
Main Author 汤江武 孙宏 姚晓红 吴逸飞 王新
Format Journal Article
LanguageChinese
Published 浙江省农业科学院植物保护与微生物研究所,浙江杭州,310021 2011
Subjects
主成分分析
消化酶活性
聚合酶链反应-变性梯度凝胶电泳
肉鸡
肠道菌群
芽孢杆菌
聚合酶链反应-变性梯度凝胶电泳
肉鸡
主成分分析
芽孢杆菌
肠道菌群
消化酶活性
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ISSN1008-9209
DOI10.3785/j.issn.1008-9209.2011.03.013

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Summary:考察枯草芽孢杆菌的芽孢在AA肉鸡肠道内的分布和随粪便的排出规律,及对肠道内细菌群落结构变化和肠道内消化酶活性的影响.试验选取240羽1日龄AA肉鸡,随机分为2组,每组6个重复,采用相同的无抗生素基础日粮饲养3 d后,对照组和试验组分别一次性灌喂无菌水或枯草芽孢杆菌的芽孢（1.5×108CFU？mL-1）各0.5 mL;在不同时间采样进行计数和酶活性测定,同时通过聚合酶链反应-变性梯度凝胶电泳（polymerase chain reaction-denaturing gradient gel electrophoresis,PCR-DGGE）技术分析此过程肉鸡粪便中细菌群落的变化.结果表明：灌喂的芽孢在十二指肠内开始萌发,以空肠的营养体数量最高,盲肠内则以芽孢为主;粪便中的芽孢数量在24 h时最高,达到7.9×104CFU？g-1,而后逐渐降低,在72 h后基本无法检出.PCR-DGGE结合主成分分析（PCA）表明：对照组与试验组鸡粪中的细菌群落结构存在较大差异,其中试验组中肠道有益菌——乳杆菌属（Lactobacillussp.）含量较对照组有明显提高;同时,灌喂芽孢12 h后,十二指肠内淀粉酶、胰蛋白酶和总蛋白酶活性,空肠内的脂肪酶活性和盲肠内的总蛋白酶活性均高于对照,差异有统计学意义（P〈0.05）.试验证实芽孢能在AA肉鸡肠道内萌发并停留超过72 h,并对提高肠道内消化酶活性和改善菌落结构发挥作用.
Bibliography:33-1247/S
TANG Jiang-wu,SUN Hong,YAO Xiao-hong,WU Yi-fei,WANG Xin（Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China）
Bacillus subtilis; broiler; intestinal flora; digestive enzyme activity; polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE）; principal component analysis（PCA）
Two hundred forty one-day-old AA broilers were randomly divided into 2 groups with 6 replicates,administrating the same antibiotics-free basal diet.After 3 d feeding,broilers of control group and experimental group were respectively dosed with 0.5 mL sterile water or spore（1.5×10^8 CFU·mL^-1）,aiming to examine the internal fate and dissemination of Bacillus subtilis spores in the intestine of AA broiler as well as its effects on changes of intestinal flora and activity of digestive enzymes.Spores were counted and enzyme activities were measured at different sampling time,while the intestinal flora was determined by using polymerase chain reaction-denaturing
ISSN:1008-9209
DOI:10.3785/j.issn.1008-9209.2011.03.013