融合表达IL-18BP和IL-4的重组腺病毒治疗对胶原诱导型关节炎小鼠血清IL-18表达的作用

R392.11; 目的:构建小鼠IL-18BP和IL-4融合表达的重组腺病毒载体(Ad mIL-18BP/mIL-4),对胶原诱导型关节炎(collagen-induced arthritis,CIA)小鼠进行局部关节基因治疗,观察小鼠血清IL-18表达水平.方珐:采用雄性DBA-1/BOM小鼠,建立胶原诱导关节炎的小鼠模型.将融合表达小鼠IL-18BP和IL4的重组腺病毒载体进行局部关节腔内注射后1、2、4周,采用酶链免疫吸附检测方法(ELISA),检测血清IL-18水平.同时设AdLacZ病毒对照组和PBS组织对照组.结果:CIA小鼠关节腔注射AdmIL-18BP/mlL-4后第1、2、4...

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Published in浙江大学学报（医学版） Vol. 40; no. 2; pp. 195 - 199
Main Authors 冷建杭, 姚航平, 沈俊娅, 王克义, 汪子伟, 卓广超
Format Journal Article
LanguageChinese
Published 杭州市第一人民医院中心实验室,浙江杭州,310006%浙江大学医学院附属第一医院传染病研究所,浙江杭州,310003 2011
Subjects
关节炎,实验性/治疗
白细胞介素18/遗传学
白细胞介素4/遗传学
基因疗法
关节炎
细胞因子
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ISSN1008-9292
DOI10.3785/j.issn.1008-9292.2011.02.013

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Abstract R392.11; 目的:构建小鼠IL-18BP和IL-4融合表达的重组腺病毒载体(Ad mIL-18BP/mIL-4),对胶原诱导型关节炎(collagen-induced arthritis,CIA)小鼠进行局部关节基因治疗,观察小鼠血清IL-18表达水平.方珐:采用雄性DBA-1/BOM小鼠,建立胶原诱导关节炎的小鼠模型.将融合表达小鼠IL-18BP和IL4的重组腺病毒载体进行局部关节腔内注射后1、2、4周,采用酶链免疫吸附检测方法(ELISA),检测血清IL-18水平.同时设AdLacZ病毒对照组和PBS组织对照组.结果:CIA小鼠关节腔注射AdmIL-18BP/mlL-4后第1、2、4周,AdmIL-18BP/mlL-4治疗组的血清IL-18平均浓度分别为(36.5±5.4)ng/L、(32.5±3.2)ng/L、(28.7±2.9)ng/L,明显低于相应时间点AdLacZ对照组[(66.2±5.1)ng/L、(69.2±4.2)ng/L、(77.7±3.9)ng/L]和PBS对照组的水平[(67.3±7.1)ng/L、(71.9±1.8)ne/L、(78.7±4.1)ng/L],P均<0.01.但治疗组血清IL-18平均水平在不同时间点无显著性差别.结论:融合表达小鼠IL-18BP和IL4的重组腺病毒基因治疗,能明显持续下调血清IL-18的表达水平,提示细胞因子基因治疗,可望为类风湿关节炎(RA)提供新的治疗策略.
AbstractList R392.11; 目的:构建小鼠IL-18BP和IL-4融合表达的重组腺病毒载体(Ad mIL-18BP/mIL-4),对胶原诱导型关节炎(collagen-induced arthritis,CIA)小鼠进行局部关节基因治疗,观察小鼠血清IL-18表达水平.方珐:采用雄性DBA-1/BOM小鼠,建立胶原诱导关节炎的小鼠模型.将融合表达小鼠IL-18BP和IL4的重组腺病毒载体进行局部关节腔内注射后1、2、4周,采用酶链免疫吸附检测方法(ELISA),检测血清IL-18水平.同时设AdLacZ病毒对照组和PBS组织对照组.结果:CIA小鼠关节腔注射AdmIL-18BP/mlL-4后第1、2、4周,AdmIL-18BP/mlL-4治疗组的血清IL-18平均浓度分别为(36.5±5.4)ng/L、(32.5±3.2)ng/L、(28.7±2.9)ng/L,明显低于相应时间点AdLacZ对照组[(66.2±5.1)ng/L、(69.2±4.2)ng/L、(77.7±3.9)ng/L]和PBS对照组的水平[(67.3±7.1)ng/L、(71.9±1.8)ne/L、(78.7±4.1)ng/L],P均<0.01.但治疗组血清IL-18平均水平在不同时间点无显著性差别.结论:融合表达小鼠IL-18BP和IL4的重组腺病毒基因治疗,能明显持续下调血清IL-18的表达水平,提示细胞因子基因治疗,可望为类风湿关节炎(RA)提供新的治疗策略.
Author 姚航平
沈俊娅
王克义
汪子伟
冷建杭
卓广超
AuthorAffiliation 杭州市第一人民医院中心实验室,浙江杭州,310006%浙江大学医学院附属第一医院传染病研究所,浙江杭州,310003
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Author_FL WANG Ke-yi
SHEN Jun-ya
LENG Jian-hang
ZHUO Guang-chao
YAO Hang-ping
WANG Zi-wei
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DocumentTitle_FL Serum IL-18 levels in mice with collagen-induced arthritis treated by recombinant adenovirus containing mIL-18BP and mIL-4 fusion gene
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Keywords 关节炎,实验性/治疗
白细胞介素18/遗传学
白细胞介素4/遗传学
基因疗法
关节炎
细胞因子
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Publisher 杭州市第一人民医院中心实验室,浙江杭州,310006%浙江大学医学院附属第一医院传染病研究所,浙江杭州,310003
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StartPage 195
Title 融合表达IL-18BP和IL-4的重组腺病毒治疗对胶原诱导型关节炎小鼠血清IL-18表达的作用
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