高效液相色谱法测定醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量

目的：建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法。方法：采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱（250 mm×4.6 mm,5μm）;流速为1.0 ml/min;柱温为30℃。测定苦参碱、氧化苦参碱采用流动相乙腈-0.2%磷酸水（V∶V=92∶8）,用三乙胺调p H至6.0,检测波长为220 nm;测定羟基红花黄色素A采用流动相乙腈-0.2%甲酸水（V∶V=15∶85）,检测波长为403 nm。结果：苦参碱、氧化苦参碱、羟基红花黄色素A的进样量分别在0.412～1.648、0.448～1.792、0.220～0.880μg范围内与峰面积具...

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Bibliographic Details
Published in中国医院用药评价与分析 Vol. 17; no. 8; pp. 1039 - 1041
Main Author 彭宏伟 郭丽华
Format Journal Article
LanguageChinese
Published 曲阜师范大学医院药剂科,山东曲阜,273165%曲阜师范大学化学与化工学院,山东曲阜,273165 2017
Subjects
含量
氧化苦参碱
羟基红花黄色素A
苦参碱
醋制消肿止痛液
高效液相色谱法
苦参碱
羟基红花黄色素A
Matrine
醋制消肿止痛液
Content
Hydroxy safflower yellow A
高效液相色谱法
氧化苦参碱
含量
HPLC
Xiaozhongzhitong vinegar liquid
Oxymatrine
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ISSN1672-2124
DOI10.14009/j.issn.1672-2124.2017.08.012

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Summary:目的：建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法。方法：采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱（250 mm×4.6 mm,5μm）;流速为1.0 ml/min;柱温为30℃。测定苦参碱、氧化苦参碱采用流动相乙腈-0.2%磷酸水（V∶V=92∶8）,用三乙胺调p H至6.0,检测波长为220 nm;测定羟基红花黄色素A采用流动相乙腈-0.2%甲酸水（V∶V=15∶85）,检测波长为403 nm。结果：苦参碱、氧化苦参碱、羟基红花黄色素A的进样量分别在0.412～1.648、0.448～1.792、0.220～0.880μg范围内与峰面积具有良好的线性关系（r分别为0.999 5、0.999 3、0.999 4）;苦参碱的平均加样回收率为98.90%,RSD为0.42%;氧化苦参碱的平均加样回收率为99.08%,RSD为0.69%;羟基红花黄色素A的平均加样回收率为99.03%,RSD为0.44%。结论：本方法操作简便、结果准确、重现性良好,可作为该制剂的质量检测标准,并为其开发应用提供了理论基础。
Bibliography:PENG Hongwei1, GUO Lihua2 （ 1. Dept. of Pharmacy, Qufu Normal University Hospital, Shandong Qufu 273165, China; 2. College of Chemistry and Chemical Engineering, Qufu Normal University, Shandong Qufu 273165, China）
OBJECTIVE： To establish the method for content determination method of matrine,oxymatrine and hydroxy safflower yellow A contents in Xiaozhongzhitong vinegar liquid by HPLC. METHODS： HPLC method was adopted,the column was Sinochrom ODS-BP（ 250 mm × 4. 6 mm,5 μm）,with flow rate of 1. 0 ml/min and column temperature of 30 ℃. Matrine and oxymatrine were determined by mobile phase acetonitrile 0. 2% and phosphoric acid water（ V ∶ V = 92∶ 8）. Triethylamine was used to adjust PH value to 6. 0,and the wave length was 220 nm. Hydroxy safflower yellow A was determined with mobile phase of acetonitrile 0. 2% methanoic acid water（ V∶ V = 15 ∶ 85） and detection wavelength of 403 nm. RESULTS： The calibration curves were linear when the sample ranges of matrine,oxymatrine and hydroxy safflower yellow A were at 0.
ISSN:1672-2124
DOI:10.14009/j.issn.1672-2124.2017.08.012