高效液相色谱法测定醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量
目的：建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法。方法：采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱（250 mm×4.6 mm,5μm）;流速为1.0 ml/min;柱温为30℃。测定苦参碱、氧化苦参碱采用流动相乙腈-0.2%磷酸水（V∶V=92∶8）,用三乙胺调p H至6.0,检测波长为220 nm;测定羟基红花黄色素A采用流动相乙腈-0.2%甲酸水（V∶V=15∶85）,检测波长为403 nm。结果：苦参碱、氧化苦参碱、羟基红花黄色素A的进样量分别在0.412～1.648、0.448～1.792、0.220～0.880μg范围内与峰面积具...
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| Published in | 中国医院用药评价与分析 Vol. 17; no. 8; pp. 1039 - 1041 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
曲阜师范大学医院药剂科,山东曲阜,273165%曲阜师范大学化学与化工学院,山东曲阜,273165
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-2124 |
| DOI | 10.14009/j.issn.1672-2124.2017.08.012 |
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| Abstract | 目的：建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法。方法：采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱（250 mm×4.6 mm,5μm）;流速为1.0 ml/min;柱温为30℃。测定苦参碱、氧化苦参碱采用流动相乙腈-0.2%磷酸水（V∶V=92∶8）,用三乙胺调p H至6.0,检测波长为220 nm;测定羟基红花黄色素A采用流动相乙腈-0.2%甲酸水（V∶V=15∶85）,检测波长为403 nm。结果：苦参碱、氧化苦参碱、羟基红花黄色素A的进样量分别在0.412～1.648、0.448～1.792、0.220～0.880μg范围内与峰面积具有良好的线性关系（r分别为0.999 5、0.999 3、0.999 4）;苦参碱的平均加样回收率为98.90%,RSD为0.42%;氧化苦参碱的平均加样回收率为99.08%,RSD为0.69%;羟基红花黄色素A的平均加样回收率为99.03%,RSD为0.44%。结论：本方法操作简便、结果准确、重现性良好,可作为该制剂的质量检测标准,并为其开发应用提供了理论基础。 |
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| AbstractList | R927.2; 目的:建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法.方法:采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱(250 mm×4.6 mm,5μm);流速为1.0 ml/min;柱温为30℃.测定苦参碱、氧化苦参碱采用流动相乙腈-0.2％磷酸水(V∶V=92∶8),用三乙胺调pH至6.0,检测波长为220 nm;测定羟基红花黄色素A采用流动相乙腈-0.2％甲酸水(V∶V=15∶85),检测波长为403 nm.结果:苦参碱、氧化苦参碱、羟基红花黄色素A的进样量分别在0.412～1.648、0.448 ～1.792、0.220 ～0.880 μg范围内与峰面积具有良好的线性关系(r分别为0.999 5、0.999 3、0.999 4);苦参碱的平均加样回收率为98.90％,RSD为0.42％;氧化苦参碱的平均加样回收率为99.08％,RSD为0.69％;羟基红花黄色素A的平均加样回收率为99.03％,RSD为0.44％.结论:本方法操作简便、结果准确、重现性良好,可作为该制剂的质量检测标准,并为其开发应用提供了理论基础. 目的：建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法。方法：采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱（250 mm×4.6 mm,5μm）;流速为1.0 ml/min;柱温为30℃。测定苦参碱、氧化苦参碱采用流动相乙腈-0.2%磷酸水（V∶V=92∶8）,用三乙胺调p H至6.0,检测波长为220 nm;测定羟基红花黄色素A采用流动相乙腈-0.2%甲酸水（V∶V=15∶85）,检测波长为403 nm。结果：苦参碱、氧化苦参碱、羟基红花黄色素A的进样量分别在0.412～1.648、0.448～1.792、0.220～0.880μg范围内与峰面积具有良好的线性关系（r分别为0.999 5、0.999 3、0.999 4）;苦参碱的平均加样回收率为98.90%,RSD为0.42%;氧化苦参碱的平均加样回收率为99.08%,RSD为0.69%;羟基红花黄色素A的平均加样回收率为99.03%,RSD为0.44%。结论：本方法操作简便、结果准确、重现性良好,可作为该制剂的质量检测标准,并为其开发应用提供了理论基础。 |
| Abstract_FL | OBJECTIVE:To establish the method for content determination method of matrine,oxymatrine and hydroxy safflower yellow A contents in Xiaozhongzhitong vinegar liquid by HPLC.METHODS:HPLC method was adopted,the column was Sinochrom ODS-BP(250 mm ×4.6 mm,5 μm),with flow rate of 1.0 ml/min and column temperature of 30 ℃,Matrine and oxymatrine were determined by mobile phase acetonitrile 0.2％ and phosphoric acid water(V∶ V=92∶ 8).Triethylamine was used to adjust PH value to 6.0,and the wave length was 220 nm.Hydroxy safflower yellow A was determined with mobile phase of acetonitrile 0.2％ methanoic acid water(V∶ V =15∶85) and detection wavelength of 403 nm.RESULTS:The calibration curves were linear when the sample ranges of matrine,oxymatrine and hydroxy safflower yellow A were at 0.412-1.648 μg,0.448-1.792 μg and 0.22-0.88 μg,respectively (r =0.999 5,r =0.999 3 and r =0.999 4).The average recovery rate and RSD of matrine was at 98.90％ and 0.42％,oxymatrine was at 99.08％ and 0.69％,and hydroxy safflower yellow A was at 99.03％ and 0.44％.CONCLUSIONS:This method is simple,rapid,accurate and suitable for the detection of quality of Xiaozhongzhitong liquid,which can provide the theoretical basis for the development and application of Xiaozhongzhitong vinegar liquid. |
| Author | 彭宏伟 郭丽华 |
| AuthorAffiliation | 曲阜师范大学医院药剂科,山东曲阜273165 曲阜师范大学化学与化工学院,山东曲阜273165 |
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| Author_FL | GUO Lihua PENG Hongwei |
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| Author_xml | – sequence: 1 fullname: 彭宏伟 郭丽华 |
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| DocumentTitleAlternate | Content Determination of Matrine, Oxymatrine and Hydroxy Safflower Yellow A Contents in Xiaozhongzhitong Vinegar Liquid by HPLC |
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| Keywords | 苦参碱 羟基红花黄色素A Matrine 醋制消肿止痛液 Content Hydroxy safflower yellow A 高效液相色谱法 氧化苦参碱 含量 HPLC Xiaozhongzhitong vinegar liquid Oxymatrine |
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| Notes | PENG Hongwei1, GUO Lihua2 （ 1. Dept. of Pharmacy, Qufu Normal University Hospital, Shandong Qufu 273165, China; 2. College of Chemistry and Chemical Engineering, Qufu Normal University, Shandong Qufu 273165, China） OBJECTIVE： To establish the method for content determination method of matrine,oxymatrine and hydroxy safflower yellow A contents in Xiaozhongzhitong vinegar liquid by HPLC. METHODS： HPLC method was adopted,the column was Sinochrom ODS-BP（ 250 mm × 4. 6 mm,5 μm）,with flow rate of 1. 0 ml/min and column temperature of 30 ℃. Matrine and oxymatrine were determined by mobile phase acetonitrile 0. 2% and phosphoric acid water（ V ∶ V = 92∶ 8）. Triethylamine was used to adjust PH value to 6. 0,and the wave length was 220 nm. Hydroxy safflower yellow A was determined with mobile phase of acetonitrile 0. 2% methanoic acid water（ V∶ V = 15 ∶ 85） and detection wavelength of 403 nm. RESULTS： The calibration curves were linear when the sample ranges of matrine,oxymatrine and hydroxy safflower yellow A were at 0. |
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| PublicationTitle | 中国医院用药评价与分析 |
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| PublicationTitle_FL | Evaluation and Analysis of Drug-Use in Hospitals of China |
| PublicationYear | 2017 |
| Publisher | 曲阜师范大学医院药剂科,山东曲阜,273165%曲阜师范大学化学与化工学院,山东曲阜,273165 |
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| Snippet | 目的：建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法。方法：采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱（250 mm×4.6 mm,5μm）;流速... R927.2; 目的:建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法.方法:采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱(250 mm×4.6 mm,5μm);流速... |
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| SubjectTerms | 含量 氧化苦参碱 羟基红花黄色素A 苦参碱 醋制消肿止痛液 高效液相色谱法 |
| Title | 高效液相色谱法测定醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量 |
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