Development of an automated platform for the optimal production of glycoconjugate vaccines expressed in Escherichia coli - Samaras, Jasmin J.; Mauri, Marta; Kay, Emily J.; Wren, Brendan W.; Micheletti, Martina (Microbial cell factories, 2021)
Keywords: ADP Ribose Transferases - metabolism; Antibiotics; Antigens; Applications software; Applied Microbiology; Automation; Automation - methods; Bacterial Toxins - metabolism; Bacterial Vaccines - biosynthesis; Bioconjugation; Biotechnology; Biotechnology - methods; Cell culture; Chemistry; Chemistry and Materials Science; Clinical trials; Collaboration; Computer applications; Conjugate vaccines; Conjugation; E coli; Enzymes; Enzymology; Epidemics; Escherichia coli; Escherichia coli - metabolism; Exotoxins - metabolism; Genetic aspects; Genetic Engineering; Glycan; Glycoconjugate vaccine; Glycosylation; High-Throughput Screening Assays - methods; Humans; Infectious diseases; Methods; Microbial genetic engineering; Microbial Genetics and Genomics; Microbial glycobiotechnology; Microbiological research; Microbiology; Peptides; PGCT; Physiological aspects; Plasmids; Pneumococcal vaccine; Pneumococcal Vaccines - biosynthesis; Pneumonia; Polysaccharides; Polysaccharides, Bacterial - metabolism; Production processes; Protein engineering; Proteins; Pseudomonas aeruginosa Exotoxin A; R&D; Research & development; Robustness (mathematics); Screening studies; Severe acute respiratory syndrome coronavirus 2; Streptococcus infections; Technology, Pharmaceutical - methods; Vaccines; Vaccines, Conjugate - biosynthesis; Virulence Factors - metabolism; Denmark; Europe; Automation; Glycoprotein; Glycoengineering; Glycoconjugate vaccine; Bioconjugation; High throughput process development; Screening studies; Pneumococcal vaccine; PGCT