An ambient-temperature storage and stabilization device performs comparably to flash-frozen collection for stool metabolomics in infants - Ramamoorthy, Sivapriya; Levy, Shira; Mohamed, Masouma; Abdelghani, Alaa; Evans, Anne M.; Miller, Luke A. D.; Mehta, Lopa; Moore, Sean; Freinkman, Elizaveta; Hourigan, Suchitra K. (BMC microbiology, 2021)
Keywords: Ambient temperature; Amino acids; Biological Microscopy; Biomedical and Life Sciences; Chromatography, Liquid; Cluster analysis; Clustering; Deoxyribonucleic acid; Diagnostic specimens; DNA; DNA, Bacterial - genetics; Ethanol; Feces; Feces - microbiology; Freezing; Freezing points; Gastrointestinal Microbiome - genetics; Gastrointestinal Microbiome - physiology; Humans; Infant; Infants; Intestinal microflora; Life Sciences; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medical examination; Metabolites; Metabolomics; Metabolomics - instrumentation; Metabolomics - methods; Methods; Microbiology; Microbiome; Microbiomes; Microbiota; Mycology; Parasitology; Preservation, Biological - instrumentation; Preservation, Biological - methods; Principal components analysis; Research Article; RNA, Ribosomal, 16S - genetics; Specimen Handling - instrumentation; Specimen Handling - methods; Stabilization; Stool; Storage; Tandem Mass Spectrometry; Temperature; Thermal properties; Virology; United States; Metabolomics; Infants; Stool; Ambient temperature; Microbiome