Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease

• Published in: BMC medical genomics Vol. 15; no. 1; pp. 262 - 10
• Main Authors: Kaiyrlykyzy, Aiym, Umbayev, Bauyrzhan, Masoud, Abdul-Razak, Baibulatova, Aida, Tsoy, Andrey, Olzhayev, Farkhad, Alzhanova, Dinara, Zholdasbekova, Gulnaz, Davletov, Kairat, Akilzhanova, Ainur, Askarova, Sholpan
• Format: Journal Article
• Language: English
• Published: London BioMed Central 16.12.2022; BioMed Central Ltd; BMC
• Subjects: Adiponectin; Adiponectin - genetics; Adiponectin - metabolism; AdipoR1; AdipoR2; Alzheimer Disease - genetics; Alzheimer's disease; Biomedical and Life Sciences; Biomedicine; Body fat; Boxes; Cardiovascular disease; Case-Control Studies; Cholesterol; Cognitive ability; Coronary vessels; CpG islands; Dementia; Dementia disorders; Development and progression; DNA methylation; Gender; Gene Expression; Genetic aspects; Gestational diabetes; Health aspects; Health care; High density lipoprotein; Human Genetics; Humans; Insulin; Leukocytes, Mononuclear - metabolism; Measurement; Metabolism; Methylation; Microarrays; Neurodegenerative diseases; Older people; Overweight; Pathogenesis; Peripheral blood mononuclear cells; Plasma; Protein hormones; Receptors, Adiponectin - genetics; Receptors, Adiponectin - metabolism; Risk factors; Serum levels; Vein & artery diseases; Kazakhstan; AdipoR2; DNA methylation; AdipoR1; Alzheimer’s disease; Adiponectin
• ISSN: 1755-8794; 1755-8794
• DOI: 10.1186/s12920-022-01420-8

Background The role of adiponectin (ADIPOQ) in Alzheimer’s disease (AD) has been documented, however, demonstrating controversial results. In this study, we investigated blood serum ADIPOQ levels, methylation of the adiponectin gene promoter, and adiponectin receptors (AdipoR1 and AdipoR2) expression in blood samples isolated from AD patients and healthy controls. Methods We performed a case–control study including 248 subjects (98 AD patients and 150 healthy controls); ADIPOQ serum levels, AdipoR1, and AdipoR2 levels in PBMC were measured by ELISA Kits, and ADIPOQ gene methylation was analyzed using methyl-specific PCR. Results Serum adiponectin levels were threefold higher in the AD group compared to the controls. We have also found a positive correlation between adiponectin and MMSE scores and high-density lipoprotein cholesterol (HDL-C) in AD patients. A significant difference in the proportion of methylation of the CpG sites at − 74 nt of the ADIPOQ gene promoter was detected in AD cases, and the levels of adiponectin in blood serum were significantly higher in methylated samples in the AD group compared to controls. The amount of AdipoR1 was significantly higher among AD subjects, while the expression of AdipoR2 did not vary between AD patients and controls. Conclusion These findings may contribute to a deeper understanding of the etiological factors leading to the development of dementia and may serve as a basis for the development of predictive biomarkers of AD.