Enhanced glycolysis contributes to the pathogenesis of experimental autoimmune neuritis

• Published in: Journal of neuroinflammation Vol. 15; no. 1; pp. 51 - 13
• Main Authors: Liu, Ru-Tao, Zhang, Min, Yang, Chun-Lin, Zhang, Peng, Zhang, Na, Du, Tong, Ge, Meng-Ru, Yue, Long-Tao, Li, Xiao-Li, Li, Heng, Duan, Rui-Sheng
• Format: Journal Article
• Language: English
• Published: London BioMed Central 21.02.2018; BioMed Central Ltd; BMC
• Subjects: 2-Deoxy-d-glucose; Biomedical and Life Sciences; Biomedicine; Experimental autoimmune neuritis; Glycolysis; Immunology; Inflammation; Nerves; Neurobiology; Neurology; Neurosciences; Glycolysis; glucose; Inflammation; 2-Deoxy; Experimental autoimmune neuritis; 2-Deoxy-D-glucose
• ISSN: 1742-2094; 1742-2094
• DOI: 10.1186/s12974-018-1095-7

Background With the recognition of the key roles of cellular metabolism in immunity, targeting metabolic pathway becomes a new strategy for autoimmune disease treatment. Guillain-Barré syndrome (GBS) is an acute immune-mediated inflammatory demyelinating disease of the peripheral nervous system, characterized by inflammatory cell infiltration. These inflammatory cells, including activated macrophages, Th1 cells, and Th17 cells, generally undergo metabolic reprogramming and rely mainly on glycolysis to exert functions. This study aimed to explore whether enhanced glycolysis contributed to the pathogenesis of experimental autoimmune neuritis (EAN), a classic model of GBS. Methods Preventive and therapeutic treatments with glycolysis inhibitor, 2-deoxy- d -glucose (2-DG), were applied to EAN rats. The effects of treatments were determined by clinical scoring, weighting, and tissue examination. Flow cytometry and ELISA were used to evaluate T cell differentiation, autoantibody level, and macrophage functions in vivo and in vitro. Results Glycolysis inhibition with 2-DG not only inhibited the initiation, but also prevented the progression of EAN, evidenced by the improved clinical scores, weight loss, inflammatory cell infiltration, and demyelination of sciatic nerves. 2-DG inhibited the differentiation of Th1, Th17, and Tfh cells but enhanced Treg cell development, accompanied with reduced autoantibody secretion. Further experiments in vitro proved glycolysis inhibition decreased the nitric oxide production and phagocytosis of macrophages and suppressed the maturation of dendritic cells (DC). Conclusion The effects of glycolysis inhibition on both innate and adaptive immune responses and the alleviation of animal clinical symptoms indicated that enhanced glycolysis contributed to the pathogenesis of EAN. Glycolysis inhibition may be a new therapy for GBS.