高效液相色谱/电喷雾-离子阱-飞行时间质谱联用法分析桂枝汤的化学成分
目的：应用高效液相色谱/电喷雾-离子阱-飞行时间质谱联用技术对桂枝汤中多种化学成分进行定性分析。方法：采用Agilent Zorbax SB-C18色谱柱（4.6 mm×250 mm,5μm）,以0.1%甲酸水溶液（A）-乙腈（B）作为流动相进行梯度洗脱;质谱采用电喷雾电离离子源,正、负离子检测模式下采集数据。结果：通过分析质谱信息与元素组成,并与对照品对照或参考相关文献数据,指认了桂枝汤中51种化合物,其中20种化合物为桂枝汤中首次报道。结论：研究结果为进一步阐明桂枝汤的药效物质提供了参考。...
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| Published in | 中国医院用药评价与分析 Vol. 18; no. 2; pp. 145 - 151 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
北京大学药学院,北京,100191
2018
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-2124 |
| DOI | 10.14009/j.issn.1672-2124.2018.02.001 |
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| Abstract | 目的：应用高效液相色谱/电喷雾-离子阱-飞行时间质谱联用技术对桂枝汤中多种化学成分进行定性分析。方法：采用Agilent Zorbax SB-C18色谱柱（4.6 mm×250 mm,5μm）,以0.1%甲酸水溶液（A）-乙腈（B）作为流动相进行梯度洗脱;质谱采用电喷雾电离离子源,正、负离子检测模式下采集数据。结果：通过分析质谱信息与元素组成,并与对照品对照或参考相关文献数据,指认了桂枝汤中51种化合物,其中20种化合物为桂枝汤中首次报道。结论：研究结果为进一步阐明桂枝汤的药效物质提供了参考。 |
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| AbstractList | 目的：应用高效液相色谱/电喷雾-离子阱-飞行时间质谱联用技术对桂枝汤中多种化学成分进行定性分析。方法：采用Agilent Zorbax SB-C18色谱柱（4.6 mm×250 mm,5μm）,以0.1%甲酸水溶液（A）-乙腈（B）作为流动相进行梯度洗脱;质谱采用电喷雾电离离子源,正、负离子检测模式下采集数据。结果：通过分析质谱信息与元素组成,并与对照品对照或参考相关文献数据,指认了桂枝汤中51种化合物,其中20种化合物为桂枝汤中首次报道。结论：研究结果为进一步阐明桂枝汤的药效物质提供了参考。 R914%R932; 目的:应用高效液相色谱/电喷雾-离子阱-飞行时间质谱联用技术对桂枝汤中多种化学成分进行定性分析.方法:采用Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm),以0.1％甲酸水溶液(A)-乙腈(B)作为流动相进行梯度洗脱;质谱采用电喷雾电离离子源,正、负离子检测模式下采集数据.结果:通过分析质谱信息与元素组成,并与对照品对照或参考相关文献数据,指认了桂枝汤中51种化合物,其中20种化合物为桂枝汤中首次报道.结论:研究结果为进一步阐明桂枝汤的药效物质提供了参考. |
| Abstract_FL | OBJECTIVE: To qualitatively identify the chemical constituents in Guizhi decoction by high performance liquid chromatography ( HPLC )/electrospray ionization-ion trap-time-of-flight mass spectrometry . METHODS:Analysis was conducted on an Agilent Zorbax SB C 18 column (4.6 mm ×250 mm, 5 μm) with 0.1％formic acid ( A)-acetonitrile ( B) as the mobile phase for gradient elution .ESI ion source was used for MS , and MS data were collected in negative and positive modes .RESULTS: Through analyzing MS data and element composition and by comparing with reference substances or with data from literature , 51 constituents from Guizhi decoction has been identified , among which 20 constituents were reported for the first time in Guizhi decoction .CONCLUSIONS:The results provide reference for further elucidation of active substance of Guizhi decoction . |
| Author | 袁鹏飞;张雯;徐风;刘广学;尚明英;蔡少青 |
| AuthorAffiliation | 北京大学药学院,北京100191 |
| AuthorAffiliation_xml | – name: 北京大学药学院,北京,100191 |
| Author_FL | SHANG Mingying LIU Guangxue YUAN Pengfei CAI Shaoqing ZHANG Wen XU Feng |
| Author_FL_xml | – sequence: 1 fullname: YUAN Pengfei – sequence: 2 fullname: ZHANG Wen – sequence: 3 fullname: XU Feng – sequence: 4 fullname: LIU Guangxue – sequence: 5 fullname: SHANG Mingying – sequence: 6 fullname: CAI Shaoqing |
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| DOI | 10.14009/j.issn.1672-2124.2018.02.001 |
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| DocumentTitleAlternate | Identification of Chemical Constituents in Guizhi Decoction by High Performance Liquid Chromatography/Electrospray Ionization-Ion Trap-Time-of-Flight Mass Spectrometry |
| DocumentTitle_FL | Identification of Chemical Constituents in Guizhi Decoction by High Performance Liquid Chromatography/Electrospray Ionization-Ion Trap-Time-of-Flight Mass Spectrometry |
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| Keywords | 高效液相色谱/电喷雾-离子阱-飞行时间质谱联用技术 Guizhi decoction Chemical constituents 桂枝汤 化学成分 HPLC/Electrospray ionization-ion trap-time-of-flight mass spectrometry |
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| Notes | OBJECTIVE： To qualitatively identify the chemical constituents in Guizhi decoction by high performance liquid chromatography（ HPLC）/electrospray ionization-ion trap-time-of-flight mass spectrometry.METHODS： Analysis was conducted on an Agilent Zorbax SB C18 column（ 4. 6 mm × 250 mm,5 μm） with 0. 1%formic acid（ A）-acetonitrile（ B） as the mobile phase for gradient elution. ESI ion source was used for MS,and MS data were collected in negative and positive modes. RESULTS： Through analyzing MS data and element composition and by comparing with reference substances or with data from literature,51 constituents from Guizhi decoction has been identified,among which 20 constituents were reported for the first time in Guizhi decoction. CONCLUSIONS：The results provide reference for further elucidation of active substance of Guizhi decoction. Guizhi decoction; HPLC/Electrospray ionization-ion trap-time-of-flight mass spectrometry; Chemical constituents YUAN Pengfei, ZHANG Wen, XU Feng, LIU Guangxue, SHANG Mingying, CAI Shao |
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| PublicationTitle | 中国医院用药评价与分析 |
| PublicationTitleAlternate | Evaluation and Analysis of Drug-use in Hospitals of China |
| PublicationTitle_FL | Evaluation and Analysis of Drug-Use in Hospitals of China |
| PublicationYear | 2018 |
| Publisher | 北京大学药学院,北京,100191 |
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| Snippet | 目的：应用高效液相色谱/电喷雾-离子阱-飞行时间质谱联用技术对桂枝汤中多种化学成分进行定性分析。方法：采用Agilent Zorbax SB-C18色谱柱（4.6 mm×250 mm,5μm）,以0.1%甲... R914%R932; 目的:应用高效液相色谱/电喷雾-离子阱-飞行时间质谱联用技术对桂枝汤中多种化学成分进行定性分析.方法:采用Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm),... |
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| SubjectTerms | 桂枝汤;高效液相色谱/电喷雾-离子阱-飞行时间质谱联用技术;化学成分 |
| Title | 高效液相色谱/电喷雾-离子阱-飞行时间质谱联用法分析桂枝汤的化学成分 |
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