Inflammatory cytokines in saliva: Early signs of metabolic disorders in chronic kidney disease. A controlled cross-sectional study

The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation. Seventy patients with clearance <20 mL/min/1.73 m 2 were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal...

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Published inOral surgery, oral medicine, oral pathology, oral radiology and endodontics Vol. 110; no. 5; pp. 597 - 604
Main Authors Thorman, Royne, Lundahl, Joachim, Yucel-Lindberg, Tülay, Hylander, Britta
Format Journal Article
LanguageEnglish
Published New York, NY Mosby, Inc 01.11.2010
Elsevier
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Online AccessGet full text
ISSN1079-2104
1528-395X
1528-395X
DOI10.1016/j.tripleo.2010.07.007

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Abstract The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation. Seventy patients with clearance <20 mL/min/1.73 m 2 were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal dialysis, and they were matched with 70 control subjects. Salivary flow rate was measured and submandibular/sublingual saliva collected. Analyses were performed for whole protein content using a protein assay, and levels of tumor necrosis factor (TNF) α, interleukin (IL) 1β, γ-interferon (γ-INF), IL-6, IL-8, IL-10, monocyte chemotactic protein (MCP) 1, and soluble intercellular adhesion molecule (sICAM) 1, by using Luminex technology. Patients with CKD had lower ( P = .03) stimulated salivary secretion rate and higher salivary whole protein concentration ( P = .002) than control subjects. Concentrations of IL-8 ( P = .03) and MCP-1 ( P = .002) were decreased and TNF-α/IL-10 ( P = .05) and IL-8/IL10 ( P = .03) ratios were decreased in CKD patients. CKD patients with low secretion levels of stimulated saliva expressed decreased levels of TNF-α ( P = .04), IL-1β ( P = .02), γ-INF ( P = .03), IL-6 ( P = .003), IL-8 ( P = .005), MCP-1 ( P = .006), and sICAM-1 ( P = .02). Salivary cytokines and secretion rates are significantly decreased in CKD patients. Further research is necessary to understand operating mechanisms and clinical implications of the down-regulation of inflammatory markers in saliva.
AbstractList The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation. Seventy patients with clearance <20 mL/min/1.73 m 2 were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal dialysis, and they were matched with 70 control subjects. Salivary flow rate was measured and submandibular/sublingual saliva collected. Analyses were performed for whole protein content using a protein assay, and levels of tumor necrosis factor (TNF) α, interleukin (IL) 1β, γ-interferon (γ-INF), IL-6, IL-8, IL-10, monocyte chemotactic protein (MCP) 1, and soluble intercellular adhesion molecule (sICAM) 1, by using Luminex technology. Patients with CKD had lower ( P = .03) stimulated salivary secretion rate and higher salivary whole protein concentration ( P = .002) than control subjects. Concentrations of IL-8 ( P = .03) and MCP-1 ( P = .002) were decreased and TNF-α/IL-10 ( P = .05) and IL-8/IL10 ( P = .03) ratios were decreased in CKD patients. CKD patients with low secretion levels of stimulated saliva expressed decreased levels of TNF-α ( P = .04), IL-1β ( P = .02), γ-INF ( P = .03), IL-6 ( P = .003), IL-8 ( P = .005), MCP-1 ( P = .006), and sICAM-1 ( P = .02). Salivary cytokines and secretion rates are significantly decreased in CKD patients. Further research is necessary to understand operating mechanisms and clinical implications of the down-regulation of inflammatory markers in saliva.
Objective The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation. Study design Seventy patients with clearance <20 mL/min/1.73 m2 were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal dialysis, and they were matched with 70 control subjects. Salivary flow rate was measured and submandibular/sublingual saliva collected. Analyses were performed for whole protein content using a protein assay, and levels of tumor necrosis factor (TNF) α, interleukin (IL) 1β, γ-interferon (γ-INF), IL-6, IL-8, IL-10, monocyte chemotactic protein (MCP) 1, and soluble intercellular adhesion molecule (sICAM) 1, by using Luminex technology. Results Patients with CKD had lower ( P = .03) stimulated salivary secretion rate and higher salivary whole protein concentration ( P = .002) than control subjects. Concentrations of IL-8 ( P = .03) and MCP-1 ( P = .002) were decreased and TNF-α/IL-10 ( P = .05) and IL-8/IL10 ( P = .03) ratios were decreased in CKD patients. CKD patients with low secretion levels of stimulated saliva expressed decreased levels of TNF-α ( P = .04), IL-1β ( P = .02), γ-INF ( P = .03), IL-6 ( P = .003), IL-8 ( P = .005), MCP-1 ( P = .006), and sICAM-1 ( P = .02). Conclusions Salivary cytokines and secretion rates are significantly decreased in CKD patients. Further research is necessary to understand operating mechanisms and clinical implications of the down-regulation of inflammatory markers in saliva.
The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation. Seventy patients with clearance <20 mL/min/1.73 m(2) were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal dialysis, and they were matched with 70 control subjects. Salivary flow rate was measured and submandibular/sublingual saliva collected. Analyses were performed for whole protein content using a protein assay, and levels of tumor necrosis factor (TNF) α, interleukin (IL) 1β, γ-interferon (γ-INF), IL-6, IL-8, IL-10, monocyte chemotactic protein (MCP) 1, and soluble intercellular adhesion molecule (sICAM) 1, by using Luminex technology. Patients with CKD had lower (P = .03) stimulated salivary secretion rate and higher salivary whole protein concentration (P = .002) than control subjects. Concentrations of IL-8 (P = .03) and MCP-1 (P = .002) were decreased and TNF-α/IL-10 (P = .05) and IL-8/IL10 (P = .03) ratios were decreased in CKD patients. CKD patients with low secretion levels of stimulated saliva expressed decreased levels of TNF-α (P = .04), IL-1β (P = .02), γ-INF (P = .03), IL-6 (P = .003), IL-8 (P = .005), MCP-1 (P = .006), and sICAM-1 (P = .02). Salivary cytokines and secretion rates are significantly decreased in CKD patients. Further research is necessary to understand operating mechanisms and clinical implications of the down-regulation of inflammatory markers in saliva.
The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation. Study design: Seventy patients with clearance 20 mL/min/1.73 m super(2 were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal dialysis, and they were matched with 70 control subjects. Salivary flow rate was measured and submandibular/sublingual saliva collected. Analyses were performed for whole protein content using a protein assay, and levels of tumor necrosis factor (TNF) alpha , interleukin (IL) 1 beta , gamma -interferon ( gamma -INF), IL-6, IL-8, IL-10, monocyte chemotactic protein (MCP) 1, and soluble intercellular adhesion molecule (sICAM) 1, by using Luminex technology. Results: Patients with CKD had lower (P = .03) stimulated salivary secretion rate and higher salivary whole protein concentration (P = .002) than control subjects. Concentrations of IL-8 (P = .03) and MCP-1 (P = .002) were decreased and TNF- alpha /IL-10 (P = .05) and IL-8/IL10 (P = .03) ratios were decreased in CKD patients. CKD patients with low secretion levels of stimulated saliva expressed decreased levels of TNF- alpha (P = .04), IL-1 beta (P = .02), gamma -INF (P = .03), IL-6 (P = .003), IL-8 (P = .005), MCP-1 (P = .006), and sICAM-1 (P = .02). Conclusions: Salivary cytokines and secretion rates are significantly decreased in CKD patients. Further research is necessary to understand operating mechanisms and clinical implications of the down-regulation of inflammatory markers in saliva.)
The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation.OBJECTIVEThe aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation.Seventy patients with clearance <20 mL/min/1.73 m(2) were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal dialysis, and they were matched with 70 control subjects. Salivary flow rate was measured and submandibular/sublingual saliva collected. Analyses were performed for whole protein content using a protein assay, and levels of tumor necrosis factor (TNF) α, interleukin (IL) 1β, γ-interferon (γ-INF), IL-6, IL-8, IL-10, monocyte chemotactic protein (MCP) 1, and soluble intercellular adhesion molecule (sICAM) 1, by using Luminex technology.STUDY DESIGNSeventy patients with clearance <20 mL/min/1.73 m(2) were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal dialysis, and they were matched with 70 control subjects. Salivary flow rate was measured and submandibular/sublingual saliva collected. Analyses were performed for whole protein content using a protein assay, and levels of tumor necrosis factor (TNF) α, interleukin (IL) 1β, γ-interferon (γ-INF), IL-6, IL-8, IL-10, monocyte chemotactic protein (MCP) 1, and soluble intercellular adhesion molecule (sICAM) 1, by using Luminex technology.Patients with CKD had lower (P = .03) stimulated salivary secretion rate and higher salivary whole protein concentration (P = .002) than control subjects. Concentrations of IL-8 (P = .03) and MCP-1 (P = .002) were decreased and TNF-α/IL-10 (P = .05) and IL-8/IL10 (P = .03) ratios were decreased in CKD patients. CKD patients with low secretion levels of stimulated saliva expressed decreased levels of TNF-α (P = .04), IL-1β (P = .02), γ-INF (P = .03), IL-6 (P = .003), IL-8 (P = .005), MCP-1 (P = .006), and sICAM-1 (P = .02).RESULTSPatients with CKD had lower (P = .03) stimulated salivary secretion rate and higher salivary whole protein concentration (P = .002) than control subjects. Concentrations of IL-8 (P = .03) and MCP-1 (P = .002) were decreased and TNF-α/IL-10 (P = .05) and IL-8/IL10 (P = .03) ratios were decreased in CKD patients. CKD patients with low secretion levels of stimulated saliva expressed decreased levels of TNF-α (P = .04), IL-1β (P = .02), γ-INF (P = .03), IL-6 (P = .003), IL-8 (P = .005), MCP-1 (P = .006), and sICAM-1 (P = .02).Salivary cytokines and secretion rates are significantly decreased in CKD patients. Further research is necessary to understand operating mechanisms and clinical implications of the down-regulation of inflammatory markers in saliva.CONCLUSIONSSalivary cytokines and secretion rates are significantly decreased in CKD patients. Further research is necessary to understand operating mechanisms and clinical implications of the down-regulation of inflammatory markers in saliva.
Author Lundahl, Joachim
Thorman, Royne
Yucel-Lindberg, Tülay
Hylander, Britta
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Issue 5
Keywords Kidney disease
Metabolic disorder
Urinary system disease
Cross sectional study
Renal failure
Cytokine
Early
Chronic kidney disease
Saliva
Language English
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Snippet The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and...
Objective The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease...
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SubjectTerms Biological and medical sciences
Case-Control Studies
Cell adhesion molecules
Chemokine CCL2 - analysis
Cross-Sectional Studies
Cytokines - analysis
Dental Caries - metabolism
Female
Humans
Inflammation Mediators - analysis
Intercellular Adhesion Molecule-1 - analysis
Interferon-gamma - analysis
Interleukin-10 - analysis
Interleukin-1beta - analysis
Interleukin-6 - analysis
Interleukin-8 - analysis
Kidneys
Male
Medical sciences
Middle Aged
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Otorhinolaryngology. Stomatology
Periapical Diseases - metabolism
Periodontitis - metabolism
Peritoneal Dialysis
Renal Dialysis
Renal failure
Renal Insufficiency, Chronic - metabolism
Renal Insufficiency, Chronic - therapy
Saliva - chemistry
Saliva - immunology
Saliva - secretion
Salivary Proteins and Peptides - analysis
Secretory Rate - physiology
Sublingual Gland - secretion
Submandibular Gland - secretion
Surgery
Tumor Necrosis Factor-alpha - analysis
Urinary system involvement in other diseases. Miscellaneous
Xerostomia - metabolism
Title Inflammatory cytokines in saliva: Early signs of metabolic disorders in chronic kidney disease. A controlled cross-sectional study
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