放射线照射的肺成纤维细胞对人脐带间充质干细胞中经典 Wnt／β-catenin 通路的影响
目的：体外研究放射线照射后的人肺成纤维细胞（ HLFs ）对人脐带间充质干细胞（HUMSCs）中经典Wnt／β-catenin信号通路的影响。方法：实验细胞分为共培养组和单纯 HUMSCs 组，共培养组为5 Gy X 射线照射后的 HLFs 通过Transwell系统与HUMSCs共培养。细胞培养3d后，蛋白质印迹法检测HUMSCs中GSK-3β、p-GSK-3β、FRAT1和胞核内β-catenin蛋白表达，酶联免疫吸附试验检测细胞培养上清液中WISP-1蛋白表达。结果：共培养组HUMSCs 中p-GSK-3β／GSK-3β相对比值（0.15±0.05）和FRAT1表达水平（0.48±0.0...
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| Published in | 浙江大学学报（医学版） no. 2; pp. 162 - 166 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
海军总医院呼吸内科，北京100048%海军总医院呼吸内科,北京,100048
2015
解放军医学院研究生队，北京100853 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1008-9292 |
| DOI | 10.3785/j.issn.1008-9292.2015.03.007 |
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| Abstract | 目的：体外研究放射线照射后的人肺成纤维细胞（ HLFs ）对人脐带间充质干细胞（HUMSCs）中经典Wnt／β-catenin信号通路的影响。方法：实验细胞分为共培养组和单纯 HUMSCs 组，共培养组为5 Gy X 射线照射后的 HLFs 通过Transwell系统与HUMSCs共培养。细胞培养3d后，蛋白质印迹法检测HUMSCs中GSK-3β、p-GSK-3β、FRAT1和胞核内β-catenin蛋白表达，酶联免疫吸附试验检测细胞培养上清液中WISP-1蛋白表达。结果：共培养组HUMSCs 中p-GSK-3β／GSK-3β相对比值（0.15±0.05）和FRAT1表达水平（0.48±0.07）较单纯HUMSCs组（0.55±0.05和1.16±0.13）明显下降，共培养组 HUMSCs 胞核内β-catenin （0.50±0.07）也较单纯HUMSCs组（2.39±0.15）明显下降；共培养组HUMSCs培养上清液中 WISP-1蛋白表达[（602.23±161.47） ng／mL ]较单纯 HUMSCs 组[（977.77±110.56）ng／mL]明显下降，差异均有统计学意义（均P＜0.05）。结论：放射线照射后的HLFs对HUMSCs中经典Wnt／β-catenin通路具有抑制作用。 |
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| AbstractList | 目的：体外研究放射线照射后的人肺成纤维细胞（ HLFs ）对人脐带间充质干细胞（HUMSCs）中经典Wnt／β-catenin信号通路的影响。方法：实验细胞分为共培养组和单纯 HUMSCs 组，共培养组为5 Gy X 射线照射后的 HLFs 通过Transwell系统与HUMSCs共培养。细胞培养3d后，蛋白质印迹法检测HUMSCs中GSK-3β、p-GSK-3β、FRAT1和胞核内β-catenin蛋白表达，酶联免疫吸附试验检测细胞培养上清液中WISP-1蛋白表达。结果：共培养组HUMSCs 中p-GSK-3β／GSK-3β相对比值（0.15±0.05）和FRAT1表达水平（0.48±0.07）较单纯HUMSCs组（0.55±0.05和1.16±0.13）明显下降，共培养组 HUMSCs 胞核内β-catenin （0.50±0.07）也较单纯HUMSCs组（2.39±0.15）明显下降；共培养组HUMSCs培养上清液中 WISP-1蛋白表达[（602.23±161.47） ng／mL ]较单纯 HUMSCs 组[（977.77±110.56）ng／mL]明显下降，差异均有统计学意义（均P＜0.05）。结论：放射线照射后的HLFs对HUMSCs中经典Wnt／β-catenin通路具有抑制作用。 R146%Q691.4; 目的：体外研究放射线照射后的人肺成纤维细胞（ HLFs ）对人脐带间充质干细胞（HUMSCs）中经典Wnt／β-catenin信号通路的影响。方法：实验细胞分为共培养组和单纯 HUMSCs 组，共培养组为5 Gy X 射线照射后的 HLFs 通过Transwell系统与HUMSCs共培养。细胞培养3d后，蛋白质印迹法检测HUMSCs中GSK-3β、p-GSK-3β、FRAT1和胞核内β-catenin蛋白表达，酶联免疫吸附试验检测细胞培养上清液中WISP-1蛋白表达。结果：共培养组HUMSCs 中p-GSK-3β／GSK-3β相对比值（0.15±0.05）和FRAT1表达水平（0.48±0.07）较单纯HUMSCs组（0.55±0.05和1.16±0.13）明显下降，共培养组 HUMSCs 胞核内β-catenin （0.50±0.07）也较单纯HUMSCs组（2.39±0.15）明显下降；共培养组HUMSCs培养上清液中 WISP-1蛋白表达[（602.23±161.47） ng／mL ]较单纯 HUMSCs 组[（977.77±110.56）ng／mL]明显下降，差异均有统计学意义（均P＜0.05）。结论：放射线照射后的HLFs对HUMSCs中经典Wnt／β-catenin通路具有抑制作用。 |
| Abstract_FL | Objective:To investigate the effect of irradiated human lung fibroblasts ( HLFs) on the canonical Wnt/β-catenin signaling pathway in human umbilical cord mesenchymal stem cells (HUMSCs).Methods:HUMSCs were cultured alone (single group) or co-cultured with HLFs exposed to 5Gy X-rays ( co-culture group ).The protein levels of GSK-3β, p-GSK-3β, FRAT1 and β-catenin in HUMSCs were examined by Western blotting 3 days after culture or co-culture.WISP-1 protein levels in conditioned medium were examined by ELISA .Results: The levels of p-GSK3β/GSK3β(0.15 ±0.05), FRAT1(0.48 ±0.07) and β-catenin(0.50 ±0.07) in co-cultured HUMSCs significantly decreased compared to those in single group ( 0 .55 ± 0.05, 1.16 ±0.13 and 2.39 ±0.15, all P<0.05).The supernatant level of WISP-1 in co-culture group was significantly decreased [(602.23 ±161.47)ng/mL], compared to the single group [(977.77 ±110.56) ng/mL, P<0.05].Conclusion: Irradiated HLFs attenuate the activation of canonical Wnt/β-catenin signaling pathway in HUMSCs in vitro. |
| Author | 张春阳 祝艳 冯华松 陈旭昕 |
| AuthorAffiliation | 解放军医学院研究生队,北京100853 海军总医院呼吸内科,北京100048 |
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| Author_FL | ZHANG Chun-yang FENG Hua-song ZHU Yan CHEN Xu-xin |
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| DocumentTitleAlternate | Effect of irradiated human lung fibroblasts on activation of canonical Wnt/β-caetnin signaling pathway in mes enchymal stem cells |
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| Keywords | β连环素/生物合成 Wnt proteins/biosynthesis 成纤维细胞 信号传导 Beta-catenin/biosynthesis 胞间信号肽类和蛋白质类 Signal transduction 间质干细胞/生理学 Mesenchymal stem cells/physiology Fibroblasts 脐带 Intercellular signaling peptides and proteins Wnt蛋白质类/生物合成 Umbilical cord 辐射性肺炎 Radiation pneumonitis |
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| Notes | 33-1248/R Objective：To investigate the effect of irradiated human lung fibroblasts （ HLFs） on the canonical Wnt/β-catenin signaling pathway in human umbilical cord mesenchymal stem cells （HUMSCs）.Methods：HUMSCs were cultured alone （single＆amp;nbsp;group） or co-cultured with HLFs exposed to 5Gy X-rays （ co-culture group ）.The protein levels of GSK-3β, p-GSK-3β, FRAT1 and β-catenin in HUMSCs were examined by Western blotting 3 days after culture or co-culture.WISP-1 protein levels in conditioned medium were examined by ELISA .Results： The levels of p-GSK3β/GSK3β（0.15 ±0.05）, FRAT1（0.48 ±0.07） and β-catenin（0.50 ±0.07） in co-cultured HUMSCs significantly decreased compared to those in single group （ 0 .55 ± 0.05, 1.16 ±0.13 and 2.39 ±0.15, all P〈0.05）.The supernatant level of WISP-1 in co-culture group was significantly decreased [（602.23 ±161.47）ng/mL], compared to the single group [（977.77 ±110.56） ng/mL, P〈0.05].Conclusion： Irradiated HLFs attenuate the activation of canonical Wnt/β-catenin signaling pathway i |
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| PublicationYear | 2015 |
| Publisher | 海军总医院呼吸内科，北京100048%海军总医院呼吸内科,北京,100048 解放军医学院研究生队，北京100853 |
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| Snippet | 目的：体外研究放射线照射后的人肺成纤维细胞（ HLFs ）对人脐带间充质干细胞（HUMSCs）中经典Wnt／β-catenin信号通路的影响。方法：实验细胞分为共培养组和单纯 HUMSCs ... R146%Q691.4; 目的：体外研究放射线照射后的人肺成纤维细胞（ HLFs ）对人脐带间充质干细胞（HUMSCs）中经典Wnt／β-catenin信号通路的影响。方法：实验细胞分为共培养组和... |
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| SubjectTerms | Wnt蛋白质类/生物合成 β连环素/生物合成 信号传导 成纤维细胞 胞间信号肽类和蛋白质类 脐带 辐射性肺炎 间质干细胞/生理学 |
| Title | 放射线照射的肺成纤维细胞对人脐带间充质干细胞中经典 Wnt／β-catenin 通路的影响 |
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