ATP生物发光法评价餐饮具的微生物污染研究

目的对ATP生物发光法与平板计数法在评价餐饮具微生物污染的检出限及检出率进行评价,并对2种方法的相关性进行研究。方法应用3家国内公司生产的ATP生物发光检测仪,分别对不同浓度的ATP标准溶液检测并绘制标准曲线;以不同浓度的大肠杆菌、金黄色葡萄球菌菌液及人工接种大肠杆菌的餐饮具作为被检品,分别应用ATP生物发光法和平板计数法进行检测,绘制ATP生物发光法对大肠杆菌、金黄色葡萄球菌检测的标准曲线,并对2种检测方法在人工污染餐饮具检测的相关性绘制曲线。结果 3家公司的ATP生物发光检测仪对ATP标准溶液、大肠杆菌和金黄色葡萄球菌的检出限差异较大;但在某一检测范围内,3家公司ATP生物发光检测仪的检测...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 7; no. 3; pp. 911 - 916
Main Author 张凤兰 徐潇 王海燕 高飞 单纯 崔生辉
Format Journal Article
LanguageChinese
Published 中国食品药品检定研究院,北京,100050 2016
Subjects
ATP生物发光法
平板计数法
微生物
餐饮具
平板计数法
ATP bioluminescence
tableware
ATP生物发光法
plate counting
餐饮具
microorganism
微生物
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ISSN2095-0381

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Summary:目的对ATP生物发光法与平板计数法在评价餐饮具微生物污染的检出限及检出率进行评价,并对2种方法的相关性进行研究。方法应用3家国内公司生产的ATP生物发光检测仪,分别对不同浓度的ATP标准溶液检测并绘制标准曲线;以不同浓度的大肠杆菌、金黄色葡萄球菌菌液及人工接种大肠杆菌的餐饮具作为被检品,分别应用ATP生物发光法和平板计数法进行检测,绘制ATP生物发光法对大肠杆菌、金黄色葡萄球菌检测的标准曲线,并对2种检测方法在人工污染餐饮具检测的相关性绘制曲线。结果 3家公司的ATP生物发光检测仪对ATP标准溶液、大肠杆菌和金黄色葡萄球菌的检出限差异较大;但在某一检测范围内,3家公司ATP生物发光检测仪的检测RLU值与ATP标准溶液、大肠杆菌和金黄色葡萄球菌量均有良好的线性相关性。3家公司ATP生物发光检测仪对人工接种大肠杆菌餐饮具的检测显示,ATP生物发光法与平板计数法有良好的线性相关性。结论3家公司的ATP生物发光法对ATP、大肠杆菌和金黄色葡萄球菌的检出限存在不同检测仪的仪器误差,且差异较大,目前,ATP生物发光法不能替代传统的平板计数法来评价餐饮具的微生物污染状况。
Bibliography:Objective To evaluate the detection limit and detection rate of the ATP bioluminescent method and the plate count method for tableware microbial contamination,and investigate the correlation between the 2 methods.Methods The standard solution with different concentrations were detected by the different ATP bioluminescent detectors produced by domestic companies（A,B,and C,respectively） and the standard curve was drawn after detection.Using different concentrations of Escherichia coli,Staphylococcus aureus bacteria liquid and artificial inoculation of E.coli tableware as goods,the ATP bioluminescence detector and the plate count method were used to detect them respectively,and then the standard curve of detection value between the 2 methods for quantity of Escherichia coli,Staphylococcus aureus and tableware in artificial pollution and the correlation curve were drawn between the 2 methods.Results The detection limit of ATP standard solution,Escherichia coli and Staphylococcus aureus were different among the 3
ISSN:2095-0381