人毛囊隆突区上皮干细胞、黑素干细胞及自发荧光存在的研究

目的·探讨人毛囊隆突区是否存在上皮干细胞、黑素干细胞及自发荧光。方法·标本收集自整形外科健康志愿者后枕部和颞部全层头皮,分离完整毛囊。利用荧光显微镜观察毛囊自发荧光情况;采用间接免疫荧光法和免疫组织化学法检测毛囊角蛋白K15和K19的表达情况;采用RT-PCR检测毛球和隆突区上皮干细胞及黑素干细胞的标志物Dct、K15和K19,以及成熟细胞标志物Tyrase、Tyrp1、MC1R、MITF的mRNA水平。结果·人毛囊隆突区细胞可观察到自发荧光;免疫荧光和免疫组织化学检测结果显示隆突区外毛根鞘表达K15和K19;RT-PCR结果显示,隆突区Dct、K15的mRNA水平显著高于毛球,K19的mRN...

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Published in上海交通大学学报(医学版) Vol. 36; no. 10; pp. 1436 - 1439
Main Author 吴桂菊 侯强 顾军 刘继勇
Format Journal Article
LanguageChinese
Published 上海市徐汇区大华医院皮肤科,上海,200237%上海长海医院皮肤科,上海,200433%上海长海医院药材科,上海,200433 2016
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ISSN1674-8115
DOI10.3969/j.issn.1674-8115.2016.10.007

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Abstract 目的·探讨人毛囊隆突区是否存在上皮干细胞、黑素干细胞及自发荧光。方法·标本收集自整形外科健康志愿者后枕部和颞部全层头皮,分离完整毛囊。利用荧光显微镜观察毛囊自发荧光情况;采用间接免疫荧光法和免疫组织化学法检测毛囊角蛋白K15和K19的表达情况;采用RT-PCR检测毛球和隆突区上皮干细胞及黑素干细胞的标志物Dct、K15和K19,以及成熟细胞标志物Tyrase、Tyrp1、MC1R、MITF的mRNA水平。结果·人毛囊隆突区细胞可观察到自发荧光;免疫荧光和免疫组织化学检测结果显示隆突区外毛根鞘表达K15和K19;RT-PCR结果显示,隆突区Dct、K15的mRNA水平显著高于毛球,K19的mRNA水平与毛球相比无显著差异,而Tyrase、Tyrp1、MITF-M、MC1R的mRNA表达水平显著低于毛球。结论·人毛囊隆突区细胞可观察到自发荧光,且有上皮干细胞与黑素干细胞的存在。
AbstractList R322.99; 目的 探讨人毛囊隆突区是否存在上皮干细胞、黑素干细胞及自发荧光.方法 标本收集自整形外科健康志愿者后枕部和颞部全层头皮,分离完整毛囊.利用荧光显微镜观察毛囊自发荧光情况;采用间接免疫荧光法和免疫组织化学法检测毛囊角蛋白K15和K19的表达情况;采用RT-PCR检测毛球和隆突区上皮干细胞及黑素干细胞的标志物Dct、K15和K19,以及成熟细胞标志物Tyrase、Tyrp1、MC1R、MITF的mRNA水平.结果 人毛囊隆突区细胞可观察到自发荧光;免疫荧光和免疫组织化学检测结果显示隆突区外毛根鞘表达K15和K19;RT-PCR结果显示,隆突区Dct、K15的mRNA水平显著高于毛球,K19的mRNA水平与毛球相比无显著差异,而Tyrase、Tyrp1、MITF-M、MC1R的mRNA表达水平显著低于毛球.结论 人毛囊隆突区细胞可观察到自发荧光,且有上皮干细胞与黑素干细胞的存在.
目的·探讨人毛囊隆突区是否存在上皮干细胞、黑素干细胞及自发荧光。方法·标本收集自整形外科健康志愿者后枕部和颞部全层头皮,分离完整毛囊。利用荧光显微镜观察毛囊自发荧光情况;采用间接免疫荧光法和免疫组织化学法检测毛囊角蛋白K15和K19的表达情况;采用RT-PCR检测毛球和隆突区上皮干细胞及黑素干细胞的标志物Dct、K15和K19,以及成熟细胞标志物Tyrase、Tyrp1、MC1R、MITF的mRNA水平。结果·人毛囊隆突区细胞可观察到自发荧光;免疫荧光和免疫组织化学检测结果显示隆突区外毛根鞘表达K15和K19;RT-PCR结果显示,隆突区Dct、K15的mRNA水平显著高于毛球,K19的mRNA水平与毛球相比无显著差异,而Tyrase、Tyrp1、MITF-M、MC1R的mRNA表达水平显著低于毛球。结论·人毛囊隆突区细胞可观察到自发荧光,且有上皮干细胞与黑素干细胞的存在。
Author 吴桂菊 侯强 顾军 刘继勇
AuthorAffiliation 上海市徐汇区大华医院皮肤科,上海200237 上海长海医院皮肤科,上海200433 上海长海医院药材科,上海200433
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Author_FL WU Gui-ju
GU Jun
LIU Ji-yong
HOU Qiang
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DocumentTitleAlternate Study on the existence of epithelial stem cells, melanocyte stem cells, and autofluorescence in the bulge region of hair follicles
DocumentTitle_FL Study on the existence of epithelial stem cells, melanocyte stem cells, and autofluorescence in the bulge region of hair follicles
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Keywords hair follicles bulge region
毛囊隆突区
autofluorescence
自发荧光区
上皮干细胞
黑素干细胞
epithelial stem cell
melanocyte stem cell
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Objective·To investigate whether there are epithelial stem cells, melanocyte stem cells, and autofluorescence in the bulge region of hair follicles. Methods·Samples were collected from the posterior occipital and temporal whole layer scalp of healthy volunteers with complete separation of hair follicles. Autofluorescence in hair follicles was observed using fluorescence microscope. Expressions of hair follicle keratins K15 and K19 were measured using indirect immunofluorescence and immunohistochemistry. RT-PCR was used to measure the mRNA expression levels of epithelial stem cell and melanocyte stem cell markers Dct, K15, and K19 and mature cell markers Tyrase, Tyrp1,MITF-M, and MC1R in hair bulbs and bulge region. Results·Autofluorescence was observed in the bulge region of hair follicles. Immunofluorescence and immunohistochemistry showed K15 and K19 were expressed in bulge region. RT-PCR showed that the mRNA expressions of Dct and K15 in bulge region were significantly higher than those in hair bu
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Publisher 上海市徐汇区大华医院皮肤科,上海,200237%上海长海医院皮肤科,上海,200433%上海长海医院药材科,上海,200433
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Snippet 目的·探讨人毛囊隆突区是否存在上皮干细胞、黑素干细胞及自发荧光。方法·标本收集自整形外科健康志愿者后枕部和颞部全层头皮,分离完整毛囊。利用荧光显微镜观察毛囊自发荧光...
R322.99; 目的 探讨人毛囊隆突区是否存在上皮干细胞、黑素干细胞及自发荧光.方法 标本收集自整形外科健康志愿者后枕部和颞部全层头皮,分离完整毛囊.利用荧光显微镜观察毛囊...
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SubjectTerms 上皮干细胞
毛囊隆突区
自发荧光区
黑素干细胞
Title 人毛囊隆突区上皮干细胞、黑素干细胞及自发荧光存在的研究
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