QTL mapping of antixenosis resistance to common cutworm (Spodoptera litura Fabricius) in wild soybean (Glycine soja)

• Published in: PloS one Vol. 12; no. 12; p. e0189440
• Main Authors: Oki, Nobuhiko, Kaga, Akito, Shimizu, Takehiko, Takahashi, Masakazu, Kono, Yuhi, Takahashi, Motoki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.12.2017; Public Library of Science (PLoS)
• Subjects: Agriculture; Animals; Antibiosis; Bioassays; Biology and Life Sciences; Breeding; Chromosomes; Crop science; Cultivars; Cutting resistance; Domestication; Ecology and Environmental Sciences; Food; Gene loci; Gene mapping; Genetic Linkage; Genetics; Germplasm; Glycine max; Glycine max - genetics; Glycine max - parasitology; Glycine soja; Helicoverpa zea; Inbreeding; Insects; Physical Sciences; Population; Pseudoplusia includens; Quantitative Trait Loci; Research and Analysis Methods; Soybeans; Spodoptera - pathogenicity; Spodoptera litura; Japan; Okinawa
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0189440

The common cutworm (CCW; Spodoptera litura Fabricius) is a serious herbivorous insect pest of soybean (Glycine max) in Asia and Oceania. Previously, we identified quantitative trait loci (QTLs) for CCW-antibiosis-resistance, CCW-1 and CCW-2, and antixenosis-resistance, qRslx1 and qRslx2, in the cultivar 'Himeshirazu'. The effects of these QTLs are useful in the breeding of CCW-resistant cultivars. In this study, we conducted an antixenosis bioassay on CCW using recombinant inbred lines derived from a cross between a wild soybean (Glycine soja) and the leading cultivar 'Fukuyutaka' to identify CCW-resistance genes in G. soja. The QTL analysis revealed six and four novel antixenosis-resistance QTLs in 2012 and 2013, respectively. Among them, the QTLs on chromosomes 2 and 7, designated qRslx4 and qRslx3, respectively, were stably detected in both years. qRslx3 exhibited the largest effect in both years, suggesting that qRslx3 can be exploited in the breeding of CCW-resistant soybean. Furthermore, qRslx3 and qRslx4 can be used, along with previously reported QTLs from 'Himeshirazu', to enhance the CCW-resistance of soybean cultivars because their chromosomal positions are unique. These new CCW-resistance QTLs from G. soja should play important roles in the breeding of CCW-resistant soybean cultivars.