固相萃取-高效液相色谱-荧光法测定辣椒制品中罗丹明B
目的建立固相萃取-高效液相色谱-荧光检测法测定辣椒制品中罗丹明B的分析方法。方法样品分别经乙腈和20%丙酮-正己烷溶液提取后,采用WelchromP-SCX强阳离子交换固相萃取小柱和Welchrom中性氧化铝固相萃取小柱进行净化,并采用Welch UltimateXB-C18色谱柱（250 mm×4.6 mm,5μm）以75%甲醇-水溶液为流动相进行分离,荧光检测器检测。结果采用WelchromP-SCX强阳离子交换小柱对辣椒制品进行前处理净化具有去除杂质效果更好,稳定性更强,方法重现性更好等优势,因而采用WelchromP-SCX小柱对辣椒制品进行前处理净化。罗丹明B在1.0-10...
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| Published in | 食品安全质量检测学报 Vol. 7; no. 6; pp. 2375 - 2380 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
浙江师范大学物理化学研究所,金华 321004
2016
浙江月旭材料科技有限公司,金华 321014%浙江月旭材料科技有限公司,金华,321014 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
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| Abstract | 目的建立固相萃取-高效液相色谱-荧光检测法测定辣椒制品中罗丹明B的分析方法。方法样品分别经乙腈和20%丙酮-正己烷溶液提取后,采用WelchromP-SCX强阳离子交换固相萃取小柱和Welchrom中性氧化铝固相萃取小柱进行净化,并采用Welch UltimateXB-C18色谱柱（250 mm×4.6 mm,5μm）以75%甲醇-水溶液为流动相进行分离,荧光检测器检测。结果采用WelchromP-SCX强阳离子交换小柱对辣椒制品进行前处理净化具有去除杂质效果更好,稳定性更强,方法重现性更好等优势,因而采用WelchromP-SCX小柱对辣椒制品进行前处理净化。罗丹明B在1.0-100.0μg/L范围具有良好的线性（r^2〉0.9995）,2种辣椒制品的回收率在91.4%-106.5%范围内,相对标准偏差RSD在2.1%-4.3%之间。结论该方法的灵敏度、准确度和精密度均符合罗丹明B的检测技术要求,且简便快速,适用于辣椒制品中罗丹明B的准确测定。 |
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| AbstractList | 目的建立固相萃取-高效液相色谱-荧光检测法测定辣椒制品中罗丹明B的分析方法。方法样品分别经乙腈和20%丙酮-正己烷溶液提取后,采用WelchromP-SCX强阳离子交换固相萃取小柱和Welchrom中性氧化铝固相萃取小柱进行净化,并采用Welch UltimateXB-C18色谱柱（250 mm×4.6 mm,5μm）以75%甲醇-水溶液为流动相进行分离,荧光检测器检测。结果采用WelchromP-SCX强阳离子交换小柱对辣椒制品进行前处理净化具有去除杂质效果更好,稳定性更强,方法重现性更好等优势,因而采用WelchromP-SCX小柱对辣椒制品进行前处理净化。罗丹明B在1.0-100.0μg/L范围具有良好的线性（r^2〉0.9995）,2种辣椒制品的回收率在91.4%-106.5%范围内,相对标准偏差RSD在2.1%-4.3%之间。结论该方法的灵敏度、准确度和精密度均符合罗丹明B的检测技术要求,且简便快速,适用于辣椒制品中罗丹明B的准确测定。 目的 建立固相萃取-高效液相色谱-荧光检测法测定辣椒制品中罗丹明B的分析方法.方法 样品分别经乙腈和20％丙酮-正己烷溶液提取后,采用Welchrom(R) P-SCX强阳离子交换固相萃取小柱和Welchrom(R)中性氧化铝固相萃取小柱进行净化,并采用Welch Ultimate(R) XB-C18色谱柱(250 mm×4.6 mm,5μm)以75％甲醇-水溶液为流动相进行分离,荧光检测器检测.结果 采用Welchrom(R) P-SCX强阳离子交换小柱对辣椒制品进行前处理净化具有去除杂质效果更好,稳定性更强,方法重现性更好等优势,因而采用Welchrom(R) P-SCX小柱对辣椒制品进行前处理净化.罗丹明B在1.0～100.0 μg/L范围具有良好的线性(r2＞ 0.9995),2种辣椒制品的回收率在91.4％～106.5％范围内,相对标准偏差RSD在2.1％～4.3％之间.结论 该方法的灵敏度、准确度和精密度均符合罗丹明B的检测技术要求,且简便快速,适用于辣椒制品中罗丹明B的准确测定. |
| Author | 薛昆鹏 金小青 余冲 赵岳星 |
| AuthorAffiliation | 浙江师范大学物理化学研究所,金华321004 浙江月旭材料科技有限公司,金华321014 |
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| Author_FL | XUE Kun-Peng YU Chong JIN Xiao-Qing ZHAO Yue-Xing |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Determination of rhodamine B in pepper products by solid phase extraction–high performance liquid chromatography-fluorescence detector |
| DocumentTitle_FL | Determination of rhodamine B in pepper products by solid phase extraction-high performance liquid chromatography-fluorescence detector |
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| Keywords | high performance liquid chromatography-fluorescence detector pepper products 罗丹明B 辣椒制品 高效液相色谱-荧光检测法 solid phase extraction 固相萃取 rhodamine B |
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| Notes | 11-5956/TS XUE Kun-Peng, JIN Xiao-Qing, YET Chong, ZHAO Yue-Xing （1. Zhejiang Normal University Institute of Physical Chemistry, Jinhua 321004, China; 2. Welch Materials （Zhejiang） Co., Ltd., Jinhua 321014, China） solid phase extraction; high performance liquid chromatography-fluorescence detector; pepper products; rhodamine B Objective To establish a method for the determination of the rhodamine B（RB） in pepper products by solid phase extraction–high performance liquid chromatography-fluorescence detector（SPE-HPLC-FLD）. Methods The sample were extracted by acetonitrile and 20% acetone-n-hexane solution, then purified and concentrated with a strong positive ion exchange SPE column（Welchrom P-SCX） and a neutral alumina SPE column（Welchrom Alumina）. The HPLC separation was performed on a Welch Ultimate XB-C18 HPLC column（250 mm × 4.6 mm, 5 μm）, eluted with a mobile phase consisting of methanol and water（75：25, V：V）. The analyzed sample was detected by fluorescence detector. The content of RB in pepper products |
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| PublicationTitle | 食品安全质量检测学报 |
| PublicationTitleAlternate | Journal of Food Safety and Quality |
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| PublicationYear | 2016 |
| Publisher | 浙江师范大学物理化学研究所,金华 321004 浙江月旭材料科技有限公司,金华 321014%浙江月旭材料科技有限公司,金华,321014 |
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| Title | 固相萃取-高效液相色谱-荧光法测定辣椒制品中罗丹明B |
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