Metabolic profiling of pregnancy: cross-sectional and longitudinal evidence

Background Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood. Methods Detailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24–49 years, 322...

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Published inBMC medicine Vol. 14; no. 1; p. 205
Main Authors Wang, Qin, Würtz, Peter, Auro, Kirsi, Mäkinen, Ville-Petteri, Kangas, Antti J., Soininen, Pasi, Tiainen, Mika, Tynkkynen, Tuulia, Jokelainen, Jari, Santalahti, Kristiina, Salmi, Marko, Blankenberg, Stefan, Zeller, Tanja, Viikari, Jorma, Kähönen, Mika, Lehtimäki, Terho, Salomaa, Veikko, Perola, Markus, Jalkanen, Sirpa, Järvelin, Marjo-Riitta, Raitakari, Olli T., Kettunen, Johannes, Lawlor, Debbie A., Ala-Korpela, Mika
Format Journal Article
LanguageEnglish
Published London BioMed Central 13.12.2016
BioMed Central Ltd
Springer Nature B.V
Subjects
Online AccessGet full text
ISSN1741-7015
1741-7015
DOI10.1186/s12916-016-0733-0

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Abstract Background Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood. Methods Detailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24–49 years, 322 pregnant) from three population-based cohorts in Finland. Circulating molecular concentrations in pregnant women were compared to those in non-pregnant women. Metabolic profiles were also reassessed for 583 women 6 years later to uncover the longitudinal metabolic changes in response to change in the pregnancy status. Results Compared to non-pregnant women, all lipoprotein subclasses and lipids were markedly increased in pregnant women. The most pronounced differences were observed for the intermediate-density, low-density and high-density lipoprotein triglyceride concentrations. Large differences were also seen for many fatty acids and amino acids. Pregnant women also had higher concentrations of low-grade inflammatory marker glycoprotein acetyls, higher concentrations of interleukin-18 and lower concentrations of interleukin-12p70. The changes in metabolic concentrations for women who were not pregnant at baseline but pregnant 6 years later (or vice versa) matched (or were mirror-images of) the cross-sectional association pattern. Cross-sectional results were consistent across the three cohorts and similar longitudinal changes were seen for 653 women in 4-year and 497 women in 10-year follow-up. For multiple metabolic measures, the changes increased in magnitude across the three trimesters. Conclusions Pregnancy initiates substantial metabolic and inflammatory changes in the mothers. Comprehensive characterisation of normal pregnancy is important for gaining understanding of the key nutrients for fetal growth and development. These findings also provide a valuable molecular reference in relation to studies of adverse pregnancy outcomes.
AbstractList Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood. Detailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24-49 years, 322 pregnant) from three population-based cohorts in Finland. Circulating molecular concentrations in pregnant women were compared to those in non-pregnant women. Metabolic profiles were also reassessed for 583 women 6 years later to uncover the longitudinal metabolic changes in response to change in the pregnancy status. Compared to non-pregnant women, all lipoprotein subclasses and lipids were markedly increased in pregnant women. The most pronounced differences were observed for the intermediate-density, low-density and high-density lipoprotein triglyceride concentrations. Large differences were also seen for many fatty acids and amino acids. Pregnant women also had higher concentrations of low-grade inflammatory marker glycoprotein acetyls, higher concentrations of interleukin-18 and lower concentrations of interleukin-12p70. The changes in metabolic concentrations for women who were not pregnant at baseline but pregnant 6 years later (or vice versa) matched (or were mirror-images of) the cross-sectional association pattern. Cross-sectional results were consistent across the three cohorts and similar longitudinal changes were seen for 653 women in 4-year and 497 women in 10-year follow-up. For multiple metabolic measures, the changes increased in magnitude across the three trimesters. Pregnancy initiates substantial metabolic and inflammatory changes in the mothers. Comprehensive characterisation of normal pregnancy is important for gaining understanding of the key nutrients for fetal growth and development. These findings also provide a valuable molecular reference in relation to studies of adverse pregnancy outcomes.
Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood.BACKGROUNDPregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood.Detailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24-49 years, 322 pregnant) from three population-based cohorts in Finland. Circulating molecular concentrations in pregnant women were compared to those in non-pregnant women. Metabolic profiles were also reassessed for 583 women 6 years later to uncover the longitudinal metabolic changes in response to change in the pregnancy status.METHODSDetailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24-49 years, 322 pregnant) from three population-based cohorts in Finland. Circulating molecular concentrations in pregnant women were compared to those in non-pregnant women. Metabolic profiles were also reassessed for 583 women 6 years later to uncover the longitudinal metabolic changes in response to change in the pregnancy status.Compared to non-pregnant women, all lipoprotein subclasses and lipids were markedly increased in pregnant women. The most pronounced differences were observed for the intermediate-density, low-density and high-density lipoprotein triglyceride concentrations. Large differences were also seen for many fatty acids and amino acids. Pregnant women also had higher concentrations of low-grade inflammatory marker glycoprotein acetyls, higher concentrations of interleukin-18 and lower concentrations of interleukin-12p70. The changes in metabolic concentrations for women who were not pregnant at baseline but pregnant 6 years later (or vice versa) matched (or were mirror-images of) the cross-sectional association pattern. Cross-sectional results were consistent across the three cohorts and similar longitudinal changes were seen for 653 women in 4-year and 497 women in 10-year follow-up. For multiple metabolic measures, the changes increased in magnitude across the three trimesters.RESULTSCompared to non-pregnant women, all lipoprotein subclasses and lipids were markedly increased in pregnant women. The most pronounced differences were observed for the intermediate-density, low-density and high-density lipoprotein triglyceride concentrations. Large differences were also seen for many fatty acids and amino acids. Pregnant women also had higher concentrations of low-grade inflammatory marker glycoprotein acetyls, higher concentrations of interleukin-18 and lower concentrations of interleukin-12p70. The changes in metabolic concentrations for women who were not pregnant at baseline but pregnant 6 years later (or vice versa) matched (or were mirror-images of) the cross-sectional association pattern. Cross-sectional results were consistent across the three cohorts and similar longitudinal changes were seen for 653 women in 4-year and 497 women in 10-year follow-up. For multiple metabolic measures, the changes increased in magnitude across the three trimesters.Pregnancy initiates substantial metabolic and inflammatory changes in the mothers. Comprehensive characterisation of normal pregnancy is important for gaining understanding of the key nutrients for fetal growth and development. These findings also provide a valuable molecular reference in relation to studies of adverse pregnancy outcomes.CONCLUSIONSPregnancy initiates substantial metabolic and inflammatory changes in the mothers. Comprehensive characterisation of normal pregnancy is important for gaining understanding of the key nutrients for fetal growth and development. These findings also provide a valuable molecular reference in relation to studies of adverse pregnancy outcomes.
Background Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood. Methods Detailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24-49 years, 322 pregnant) from three population-based cohorts in Finland. Circulating molecular concentrations in pregnant women were compared to those in non-pregnant women. Metabolic profiles were also reassessed for 583 women 6 years later to uncover the longitudinal metabolic changes in response to change in the pregnancy status. Results Compared to non-pregnant women, all lipoprotein subclasses and lipids were markedly increased in pregnant women. The most pronounced differences were observed for the intermediate-density, low-density and high-density lipoprotein triglyceride concentrations. Large differences were also seen for many fatty acids and amino acids. Pregnant women also had higher concentrations of low-grade inflammatory marker glycoprotein acetyls, higher concentrations of interleukin-18 and lower concentrations of interleukin-12p70. The changes in metabolic concentrations for women who were not pregnant at baseline but pregnant 6 years later (or vice versa) matched (or were mirror-images of) the cross-sectional association pattern. Cross-sectional results were consistent across the three cohorts and similar longitudinal changes were seen for 653 women in 4-year and 497 women in 10-year follow-up. For multiple metabolic measures, the changes increased in magnitude across the three trimesters. Conclusions Pregnancy initiates substantial metabolic and inflammatory changes in the mothers. Comprehensive characterisation of normal pregnancy is important for gaining understanding of the key nutrients for fetal growth and development. These findings also provide a valuable molecular reference in relation to studies of adverse pregnancy outcomes. Keywords: Pregnancy, Trimesters, Postpartum, Metabolomics, Cytokines, Lipoprotein lipids, Fatty acids, Amino acids, Hormones, Inflammation, Metabolic networks
Background Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood. Methods Detailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24-49 years, 322 pregnant) from three population-based cohorts in Finland. Circulating molecular concentrations in pregnant women were compared to those in non-pregnant women. Metabolic profiles were also reassessed for 583 women 6 years later to uncover the longitudinal metabolic changes in response to change in the pregnancy status. Results Compared to non-pregnant women, all lipoprotein subclasses and lipids were markedly increased in pregnant women. The most pronounced differences were observed for the intermediate-density, low-density and high-density lipoprotein triglyceride concentrations. Large differences were also seen for many fatty acids and amino acids. Pregnant women also had higher concentrations of low-grade inflammatory marker glycoprotein acetyls, higher concentrations of interleukin-18 and lower concentrations of interleukin-12p70. The changes in metabolic concentrations for women who were not pregnant at baseline but pregnant 6 years later (or vice versa) matched (or were mirror-images of) the cross-sectional association pattern. Cross-sectional results were consistent across the three cohorts and similar longitudinal changes were seen for 653 women in 4-year and 497 women in 10-year follow-up. For multiple metabolic measures, the changes increased in magnitude across the three trimesters. Conclusions Pregnancy initiates substantial metabolic and inflammatory changes in the mothers. Comprehensive characterisation of normal pregnancy is important for gaining understanding of the key nutrients for fetal growth and development. These findings also provide a valuable molecular reference in relation to studies of adverse pregnancy outcomes.
Background Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood. Methods Detailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24–49 years, 322 pregnant) from three population-based cohorts in Finland. Circulating molecular concentrations in pregnant women were compared to those in non-pregnant women. Metabolic profiles were also reassessed for 583 women 6 years later to uncover the longitudinal metabolic changes in response to change in the pregnancy status. Results Compared to non-pregnant women, all lipoprotein subclasses and lipids were markedly increased in pregnant women. The most pronounced differences were observed for the intermediate-density, low-density and high-density lipoprotein triglyceride concentrations. Large differences were also seen for many fatty acids and amino acids. Pregnant women also had higher concentrations of low-grade inflammatory marker glycoprotein acetyls, higher concentrations of interleukin-18 and lower concentrations of interleukin-12p70. The changes in metabolic concentrations for women who were not pregnant at baseline but pregnant 6 years later (or vice versa) matched (or were mirror-images of) the cross-sectional association pattern. Cross-sectional results were consistent across the three cohorts and similar longitudinal changes were seen for 653 women in 4-year and 497 women in 10-year follow-up. For multiple metabolic measures, the changes increased in magnitude across the three trimesters. Conclusions Pregnancy initiates substantial metabolic and inflammatory changes in the mothers. Comprehensive characterisation of normal pregnancy is important for gaining understanding of the key nutrients for fetal growth and development. These findings also provide a valuable molecular reference in relation to studies of adverse pregnancy outcomes.
Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood. Detailed molecular profiles (87 metabolic measures and 37 cytokines) were measured for up to 4260 women (24-49 years, 322 pregnant) from three population-based cohorts in Finland. Circulating molecular concentrations in pregnant women were compared to those in non-pregnant women. Metabolic profiles were also reassessed for 583 women 6 years later to uncover the longitudinal metabolic changes in response to change in the pregnancy status. Compared to non-pregnant women, all lipoprotein subclasses and lipids were markedly increased in pregnant women. The most pronounced differences were observed for the intermediate-density, low-density and high-density lipoprotein triglyceride concentrations. Large differences were also seen for many fatty acids and amino acids. Pregnant women also had higher concentrations of low-grade inflammatory marker glycoprotein acetyls, higher concentrations of interleukin-18 and lower concentrations of interleukin-12p70. The changes in metabolic concentrations for women who were not pregnant at baseline but pregnant 6 years later (or vice versa) matched (or were mirror-images of) the cross-sectional association pattern. Cross-sectional results were consistent across the three cohorts and similar longitudinal changes were seen for 653 women in 4-year and 497 women in 10-year follow-up. For multiple metabolic measures, the changes increased in magnitude across the three trimesters. Pregnancy initiates substantial metabolic and inflammatory changes in the mothers. Comprehensive characterisation of normal pregnancy is important for gaining understanding of the key nutrients for fetal growth and development. These findings also provide a valuable molecular reference in relation to studies of adverse pregnancy outcomes.
ArticleNumber 205
Audience Academic
Author Ala-Korpela, Mika
Viikari, Jorma
Järvelin, Marjo-Riitta
Kangas, Antti J.
Salmi, Marko
Kähönen, Mika
Soininen, Pasi
Jalkanen, Sirpa
Mäkinen, Ville-Petteri
Tiainen, Mika
Würtz, Peter
Lawlor, Debbie A.
Tynkkynen, Tuulia
Santalahti, Kristiina
Zeller, Tanja
Lehtimäki, Terho
Raitakari, Olli T.
Jokelainen, Jari
Blankenberg, Stefan
Auro, Kirsi
Perola, Markus
Kettunen, Johannes
Salomaa, Veikko
Wang, Qin
Author_xml – sequence: 1
  givenname: Qin
  surname: Wang
  fullname: Wang, Qin
  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu, NMR Metabolomics Laboratory, School of Pharmacy, University of Eastern Finland
– sequence: 2
  givenname: Peter
  surname: Würtz
  fullname: Würtz, Peter
  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu
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  givenname: Kirsi
  surname: Auro
  fullname: Auro, Kirsi
  organization: National Institute for Health and Welfare, Institute for Molecular Medicine (FIMM), University of Helsinki, Department of Obstetrics and Gynecology, Helsinki University Central Hospital and University of Helsinki
– sequence: 4
  givenname: Ville-Petteri
  surname: Mäkinen
  fullname: Mäkinen, Ville-Petteri
  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu, Heart Health Theme, South Australian Health and Medical Research Institute, School of Biological Sciences, University of Adelaide
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  givenname: Antti J.
  surname: Kangas
  fullname: Kangas, Antti J.
  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu
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  givenname: Pasi
  surname: Soininen
  fullname: Soininen, Pasi
  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu, NMR Metabolomics Laboratory, School of Pharmacy, University of Eastern Finland
– sequence: 7
  givenname: Mika
  surname: Tiainen
  fullname: Tiainen, Mika
  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu, NMR Metabolomics Laboratory, School of Pharmacy, University of Eastern Finland
– sequence: 8
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  surname: Tynkkynen
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  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu, NMR Metabolomics Laboratory, School of Pharmacy, University of Eastern Finland
– sequence: 9
  givenname: Jari
  surname: Jokelainen
  fullname: Jokelainen, Jari
  organization: Center for Life Course Health Research and Biocenter Oulu, University of Oulu, Unit of Primary Care, Oulu University Hospital
– sequence: 10
  givenname: Kristiina
  surname: Santalahti
  fullname: Santalahti, Kristiina
  organization: Department of Medical Microbiology and Immunology, and MediCity Research Laboratory, University of Turku
– sequence: 11
  givenname: Marko
  surname: Salmi
  fullname: Salmi, Marko
  organization: Department of Medical Microbiology and Immunology, and MediCity Research Laboratory, University of Turku
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  givenname: Stefan
  surname: Blankenberg
  fullname: Blankenberg, Stefan
  organization: Clinic for General and Interventional Cardiology, University Heart Center Hamburg, German Center for Cardiovascular Research (DZHK e.V.), partner site Hamburg
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  givenname: Tanja
  surname: Zeller
  fullname: Zeller, Tanja
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  givenname: Jorma
  surname: Viikari
  fullname: Viikari, Jorma
  organization: Department of Medicine, University of Turku, Division of Medicine, Turku University Hospital
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  givenname: Mika
  surname: Kähönen
  fullname: Kähönen, Mika
  organization: Department of Clinical Physiology, University of Tampere and Tampere University Hospital
– sequence: 16
  givenname: Terho
  surname: Lehtimäki
  fullname: Lehtimäki, Terho
  organization: Department of Clinical Chemistry, Fimlab Laboratories, School of Medicine, University of Tampere
– sequence: 17
  givenname: Veikko
  surname: Salomaa
  fullname: Salomaa, Veikko
  organization: National Institute for Health and Welfare
– sequence: 18
  givenname: Markus
  surname: Perola
  fullname: Perola, Markus
  organization: National Institute for Health and Welfare, Institute for Molecular Medicine (FIMM), University of Helsinki, Estonian Genome Center, University of Tartu
– sequence: 19
  givenname: Sirpa
  surname: Jalkanen
  fullname: Jalkanen, Sirpa
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  givenname: Marjo-Riitta
  surname: Järvelin
  fullname: Järvelin, Marjo-Riitta
  organization: Center for Life Course Health Research and Biocenter Oulu, University of Oulu, Unit of Primary Care, Oulu University Hospital, Department of Epidemiology and Biostatistics, MRC-PHE Centre for Environment and Health, School of Public Health, Imperial College London
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  givenname: Olli T.
  surname: Raitakari
  fullname: Raitakari, Olli T.
  organization: Research Centre of Applied and Preventive Cardiovascular Medicine, University of Turku, Department of Clinical Physiology and Nuclear Medicine, Turku University Hospital
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  givenname: Johannes
  surname: Kettunen
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  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu, NMR Metabolomics Laboratory, School of Pharmacy, University of Eastern Finland, National Institute for Health and Welfare
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  givenname: Debbie A.
  surname: Lawlor
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  givenname: Mika
  surname: Ala-Korpela
  fullname: Ala-Korpela, Mika
  email: mika.ala-korpela@computationalmedicine.fi
  organization: Computational Medicine, Faculty of Medicine, University of Oulu and Biocenter Oulu, NMR Metabolomics Laboratory, School of Pharmacy, University of Eastern Finland, Medical Research Council Integrative Epidemiology Unit at the University of Bristol, School of Social and Community Medicine, University of Bristol
BackLink https://www.ncbi.nlm.nih.gov/pubmed/27955712$$D View this record in MEDLINE/PubMed
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Issue 1
Keywords Pregnancy
Metabolomics
Cytokines
Metabolic networks
Amino acids
Inflammation
Trimesters
Hormones
Lipoprotein lipids
Fatty acids
Postpartum
Language English
License Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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Snippet Background Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely...
Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely understood....
Background Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely...
Pregnancy triggers well-known alterations in maternal glucose and lipid balance but its overall effects on systemic metabolism remain incompletely...
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StartPage 205
SubjectTerms Adult
Biomedicine
Blood pressure
Body mass index
Breastfeeding & lactation
Cross-Sectional Studies
Diabetes
Female
Fetuses
Finland
Gestational age
Glucose
Health aspects
Humans
Insulin
Lipids
Measurement
Medicine
Medicine & Public Health
Metabolism
Metabolomics - methods
Middle Aged
Physiological aspects
Pregnancy
Pregnancy - metabolism
Pregnant women
Research Article
Young Adult
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Title Metabolic profiling of pregnancy: cross-sectional and longitudinal evidence
URI https://link.springer.com/article/10.1186/s12916-016-0733-0
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