鼠李糖乳杆菌对黄曲霉毒素B1的降解及对肝损伤的保护作用

目的分析鼠李糖乳杆菌（Lactobacillus rhamnosus GG,LGG）对黄曲霉毒素B1（aflatoxin B1,AFB1）的降解能力,阐述LGG对由AFB1引起的肝损伤的保护作用。方法利用LGG培养液、LGG上清液组、LGG菌体、热处理后上清液和热处理后LGG菌体处理AFB1,利用高效液相色谱法测定AFB1的残留量,分析LGG对AFB的降解能力。利用低、中和高剂量的LGG菌液灌胃由AFB1引起肝损伤的大鼠,并以空白和阳性作为对照组,测定大鼠的血清肝功能及肝组织抗氧化指标,分析LGG对肝损伤的保护作用。结果 AFB1降解实验表明LGG菌液对AFB1具有显著的降解作用（P〈0.05...

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Bibliographic Details
Published in食品安全质量检测学报 Vol. 7; no. 10; pp. 4169 - 4173
Main Author 周旻昱 周秀芳
Format Journal Article
LanguageChinese
Published 内蒙古医科大学附属医院,呼和浩特,010050 2016
Subjects
保护作用
肝损伤
降解
黄曲霉毒素B1
鼠李糖乳杆菌
liver injury
aflatoxin B1
降解
protective effect
黄曲霉毒素B1
鼠李糖乳杆菌
保护作用
degradation
肝损伤
Lactobacillus rhamnosus GG
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ISSN2095-0381

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Summary:目的分析鼠李糖乳杆菌（Lactobacillus rhamnosus GG,LGG）对黄曲霉毒素B1（aflatoxin B1,AFB1）的降解能力,阐述LGG对由AFB1引起的肝损伤的保护作用。方法利用LGG培养液、LGG上清液组、LGG菌体、热处理后上清液和热处理后LGG菌体处理AFB1,利用高效液相色谱法测定AFB1的残留量,分析LGG对AFB的降解能力。利用低、中和高剂量的LGG菌液灌胃由AFB1引起肝损伤的大鼠,并以空白和阳性作为对照组,测定大鼠的血清肝功能及肝组织抗氧化指标,分析LGG对肝损伤的保护作用。结果 AFB1降解实验表明LGG菌液对AFB1具有显著的降解作用（P〈0.05）,36 h能够降解（92.01±2.02）%的AFB1。降解作用是由菌体和LGG的代谢产物共同作用的结果。大鼠血清肝功能及肝组织抗氧化指标结果表明低、中和高剂量的LGG灌胃给药均能显著改善因AFB1引起的大鼠肝功能和肝组织抗氧化指标异常（P〈0.05）。结论 LGG对AFB1具有良好的降解作用,且能够有效抑制因AFB1引起的肝损伤。
Bibliography:Objective To analyze the effects of Lactobacillus rhamnosus GG（LGG） on the degradation of aflatoxin B1（AFB1） and reveal the protective effect of LGG on the liver injury caused by AFB1.Methods The AFB]was treated with LGG culture,supernatant of LGG,cell of LGG,supernatant of LGG with heat treatment and cell of LGG with heat treatment.The residues of AFB1 were determined by high performance liquid chromatography（HPLC）,which could analyze the effects of LGG on the degradation of AFB].The liver injury rats caused by AFB）were lavaged using low,medium and high dose of LGG culture.The serum liver function and liver tissue antioxidant index of rats were determined to reveal the protective effect of LGG on the liver injury.Results The degradation experiment of AFB]showed that LGG thallus had a significant degradation effect（P〈0.05） on AFB｜.With a treatment for 36 h,the degradation rate was（92.01±2.02）%.The degradation was carried out by LGG thallus and metabolites.The serum liver function and liver tissue antioxidant
ISSN:2095-0381