常见肉类中鸭源性成分荧光PCR检测方法的建立
目的通过筛选引物、探针体系,优化PCR反应条件,建立常见肉品中鸭源性DNA的荧光PCR检测方法。方法针对线粒体基因设计50对引物探针,以鸭DNA为模板,利用PCR和荧光PCR方法筛选引物探针并确定反应条件,进行灵敏度、特异性和稳定性试验,最终确定荧光定量PCR方法的引物、探针及反应条件。结果当样本含量为0.1%时,本方法仍可特异、灵敏地检出鸭肉成分,检测Ct值可稳定在20以下。结论建立了常见肉品中鸭源性DNA的荧光PCR检测方法,可用于肉类制品中鸭源性成分的鉴定。...
Saved in:
| Published in | 食品安全质量检测学报 Vol. 7; no. 2; pp. 823 - 828 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
国家食品安全风险评估中心,北京,100021
2016
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-0381 |
Cover
| Abstract | 目的通过筛选引物、探针体系,优化PCR反应条件,建立常见肉品中鸭源性DNA的荧光PCR检测方法。方法针对线粒体基因设计50对引物探针,以鸭DNA为模板,利用PCR和荧光PCR方法筛选引物探针并确定反应条件,进行灵敏度、特异性和稳定性试验,最终确定荧光定量PCR方法的引物、探针及反应条件。结果当样本含量为0.1%时,本方法仍可特异、灵敏地检出鸭肉成分,检测Ct值可稳定在20以下。结论建立了常见肉品中鸭源性DNA的荧光PCR检测方法,可用于肉类制品中鸭源性成分的鉴定。 |
|---|---|
| AbstractList | 目的通过筛选引物、探针体系,优化PCR反应条件,建立常见肉品中鸭源性DNA的荧光PCR检测方法。方法针对线粒体基因设计50对引物探针,以鸭DNA为模板,利用PCR和荧光PCR方法筛选引物探针并确定反应条件,进行灵敏度、特异性和稳定性试验,最终确定荧光定量PCR方法的引物、探针及反应条件。结果当样本含量为0.1%时,本方法仍可特异、灵敏地检出鸭肉成分,检测Ct值可稳定在20以下。结论建立了常见肉品中鸭源性DNA的荧光PCR检测方法,可用于肉类制品中鸭源性成分的鉴定。 目的 通过筛选引物、探针体系,优化PCR反应条件,建立常见肉品中鸭源性DNA的荧光PCR检测方法.方法 针对线粒体基因设计50对引物探针,以鸭DNA为模板,利用PCR和荧光PCR方法筛选引物探针并确定反应条件,进行灵敏度、特异性和稳定性试验,最终确定荧光定量PCR方法的引物、探针及反应条件.结果 当样本含量为0.1％时,本方法仍可特异、灵敏地检出鸭肉成分,检测Ct值可稳定在20以下.结论 建立了常见肉品中鸭源性DNA的荧光PCR检测方法,可用于肉类制品中鸭源性成分的鉴定. |
| Author | 王佳慧 吴爽 李楠 宋思佳 王晓菲 李凤琴 江涛 |
| AuthorAffiliation | 国家食品安全风险评估中心,北京100021 |
| AuthorAffiliation_xml | – name: 国家食品安全风险评估中心,北京,100021 |
| Author_FL | SONG Si-Jia WANG Jia-Hui WU Shuang WANG Xiao-Fei LI Feng-Qin JIANG Tao LI Nan |
| Author_FL_xml | – sequence: 1 fullname: WANG Jia-Hui – sequence: 2 fullname: WU Shuang – sequence: 3 fullname: LI Nan – sequence: 4 fullname: SONG Si-Jia – sequence: 5 fullname: WANG Xiao-Fei – sequence: 6 fullname: LI Feng-Qin – sequence: 7 fullname: JIANG Tao |
| Author_xml | – sequence: 1 fullname: 王佳慧 吴爽 李楠 宋思佳 王晓菲 李凤琴 江涛 |
| BookMark | eNotzT9Lw0AcxvEbKlhr34SrBO5-l1xy4CLBf1BQpHu4uyS1pSZtg4hOVbS0FKGLDq4OGaSDiBDbt2Mu9V0YqNOzfHi-W6gSxVFQQVXA3DIwdcgmqidJW2JsE-AMWBXt5Vm2Su9W95PiY_mTzX-zuV7M9DDV41k-Hq2e0vxxcuae67eh_prql2_9-Vy8PuTLRfE-3UYboegmQf1_a6h5eNB0j43G6dGJu98wFKOmQU2Jfa4UB2lLCcQEH3CoTGoFjg_gCMqcULHA4ZQoCJXlm9KybUY4BIQLSWtod317LaJQRC2vE18NojLoJT3Rv-12VCcBTBgGzMxS76y1uoijVr9d-t6gfSkGNx5jDtgA1KJ_hF9mhg |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W92 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DatabaseName | 中文期刊服务平台 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库-工程技术 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Diet & Clinical Nutrition |
| DocumentTitleAlternate | Establishment of real-time fluorescence quantitative PCR method for identifying duck ingredients from edible meat |
| DocumentTitle_FL | Establishment of real-time fluorescence quantitative PCR method for identifying duck ingredients from edible meat |
| EndPage | 828 |
| ExternalDocumentID | spaqzljcjs201602064 668272235 |
| GroupedDBID | -02 2B. 2C0 2RA 92H 92I 92L ALMA_UNASSIGNED_HOLDINGS CCEZO CDRFL CQIGP C~G DYU OK1 OZF TCJ TGT U1G U5L W92 ~WA 4A8 93N PSX |
| ID | FETCH-LOGICAL-c634-34b0d9cc92b7bb2142d20fc435e8d228a368fc6e8931c2fc5d4b5776192e19ab3 |
| ISSN | 2095-0381 |
| IngestDate | Thu May 29 04:00:39 EDT 2025 Wed Feb 14 10:20:38 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | 线粒体DNA 实时荧光定量PCR duck mitochondrial DNA real-time fluorescence quantitative PCR 鸭肉 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c634-34b0d9cc92b7bb2142d20fc435e8d228a368fc6e8931c2fc5d4b5776192e19ab3 |
| Notes | Objective To establish a real-time fluorescence quantitative PCR method for identifying the hidden duck ingredients in other meats through screening of primers and probe system and optimizing of PCR reaction conditions. Methods Totally fifty primer pairs and probes were designed according to sequence of the cytochrome b gene from the duck. Primers and probe were selected and the conditions of the PCR were confirmed. Specificity and sensitivity of the method were evaluated. Results Duck ingredients could be identified specifically and sensitively, even the content percentage was 0.1%. The Ct value was below 20. Conclusion The real-time fluorescence quantitative PCR method for identification of duck has high specificity and sensitivity. This method can be used for identifying hidden duck ingredients in other meats. 11-5956/TS real-time fluorescence quantitative PCR; duck; mitochondrial DNA WANG Jia-Hui;WU Shuang;LI Nan;SONG Si-Jia;WANG Xiao-Fei;LI Feng-Qin;JIANG Tao;China National Center for Food Safety Risk Asses |
| PageCount | 6 |
| ParticipantIDs | wanfang_journals_spaqzljcjs201602064 chongqing_primary_668272235 |
| PublicationCentury | 2000 |
| PublicationDate | 2016 |
| PublicationDateYYYYMMDD | 2016-01-01 |
| PublicationDate_xml | – year: 2016 text: 2016 |
| PublicationDecade | 2010 |
| PublicationTitle | 食品安全质量检测学报 |
| PublicationTitleAlternate | Journal of Food Safety and Quality |
| PublicationTitle_FL | Journal of Food Safety & Quality |
| PublicationYear | 2016 |
| Publisher | 国家食品安全风险评估中心,北京,100021 |
| Publisher_xml | – name: 国家食品安全风险评估中心,北京,100021 |
| SSID | ssib007129626 ssib017479571 ssib051376490 ssib007891667 ssj0001257730 |
| Score | 2.044784 |
| Snippet | 目的通过筛选引物、探针体系,优化PCR反应条件,建立常见肉品中鸭源性DNA的荧光PCR检测方法。方法针对线粒体基因设计50对引物探针,以鸭DNA为模板,利用PCR和荧光PCR方法筛选引... 目的 通过筛选引物、探针体系,优化PCR反应条件,建立常见肉品中鸭源性DNA的荧光PCR检测方法.方法 针对线粒体基因设计50对引物探针,以鸭DNA为模板,利用PCR和荧光PCR方法筛选... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 823 |
| SubjectTerms | 实时荧光定量PCR 线粒体DNA 鸭肉 |
| Title | 常见肉类中鸭源性成分荧光PCR检测方法的建立 |
| URI | http://lib.cqvip.com/qk/71822X/201602/668272235.html https://d.wanfangdata.com.cn/periodical/spaqzljcjs201602064 |
| Volume | 7 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVCAB databaseName: Nutrition and Food Sciences Database issn: 2095-0381 databaseCode: DYU dateStart: 20120101 customDbUrl: isFulltext: true dateEnd: 99991231 titleUrlDefault: https://www.cabidigitallibrary.org/product/zd omitProxy: true ssIdentifier: ssj0001257730 providerName: CAB International |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR09b9QwNCqdWBCfohTQDTVbUC62Y1ticS45VUhUCLVSmU6Jk2upqiul16VTQYBaVUhdYGBl6IA6IIR0tH-HS8u_4D0ndw1Sh4KURE_Pz37v-cX2s5NnO86MkZnIszx3uaLcZUZ5rjLMd5WUxqMso9RgoPCTuWB2gT1e5IsTl1Zrfy1t9tOHZuvcuJL_sSrgwK4YJfsPlh0XCgiAwb7wBAvD80I2JjEnocQrlkQL8AoRkD6RisSChE0ShiRmSKAjEqsREJBQE-UhID3MiIC0GI6ADGw5kU0CDFzqaesZkmmKWbAEQIYIKICVxVCiOPJVmkhmZQuREWB0CMR1PxiFgaJUG8kUtZJzomMrueWorVIhs4AiErRrnycAR410YFXQRPPRG4RsZWxJoAIilA5JgFyckXDUGXggLTCLzlJArwjzI0fI49Xz6LhSHf8Sicbl1xdQyshO-7JbLiGSlFnD4GLKWxqUQBEFsLI2btuaBYYtEno100JdQxKFBmNLaBElbCrUSAuQ-HmlDBEvO33fw4h5Wh5jMxqhRK0h-rXRRpah2iPHpYqy_3v7cKBc31pdMSsbqDtMDgLcPBfcUlypiJ4vjDtfAZ5fUPPFhYSZw5lvypswFLHqk2q5gMmFsGf5jGXG_UmW13pL6-B12SC4XjfpLdX8tfmrzpVqotXQZau55kxsLV93pqIXeb_xoFHthrvamBsdRnHDeTQcDE4PXp--2T35dvxrcPh7cFgc7RfbB8XO_nDn_emHg-G7XWgFxZft4sde8eln8f3jyee3w-Ojk697N535djzfmnWrs0VcE1DmUpZ6mTJG-alIU9x2MPO9roG5Qy4z35cJDWTXBDl4803jdw3PWArq4mpD3lRJSm85k721Xn7bafBu7jdzQwXNGeM8SXgisoTCzfKg66dTzvS4Ujovyy1kOkEgfQGeOZ9yZqpq6lQdy0bnHJPduRjZtHMZ4XKJ8K4z2X-1md8Dp7mf3rfG_gPfzJW_ |
| linkProvider | CAB International |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%B8%B8%E8%A7%81%E8%82%89%E7%B1%BB%E4%B8%AD%E9%B8%AD%E6%BA%90%E6%80%A7%E6%88%90%E5%88%86%E8%8D%A7%E5%85%89PCR%E6%A3%80%E6%B5%8B%E6%96%B9%E6%B3%95%E7%9A%84%E5%BB%BA%E7%AB%8B&rft.jtitle=%E9%A3%9F%E5%93%81%E5%AE%89%E5%85%A8%E8%B4%A8%E9%87%8F%E6%A3%80%E6%B5%8B%E5%AD%A6%E6%8A%A5&rft.au=%E7%8E%8B%E4%BD%B3%E6%85%A7&rft.au=%E5%90%B4%E7%88%BD&rft.au=%E6%9D%8E%E6%A5%A0&rft.au=%E5%AE%8B%E6%80%9D%E4%BD%B3&rft.date=2016&rft.pub=%E5%9B%BD%E5%AE%B6%E9%A3%9F%E5%93%81%E5%AE%89%E5%85%A8%E9%A3%8E%E9%99%A9%E8%AF%84%E4%BC%B0%E4%B8%AD%E5%BF%83%2C%E5%8C%97%E4%BA%AC%2C100021&rft.issn=2095-0381&rft.volume=7&rft.issue=2&rft.spage=823&rft.epage=828&rft.externalDocID=spaqzljcjs201602064 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F71822X%2F71822X.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fspaqzljcjs%2Fspaqzljcjs.jpg |