Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre

• Published in: Nature communications Vol. 13; no. 1; pp. 6979 - 10
• Main Authors: Harding, Katie J., Turk-Kubo, Kendra A., Mak, Esther Wing Kwan, Weber, Peter K., Mayali, Xavier, Zehr, Jonathan P.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.11.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326/41/2535; 704/829/826; Bicarbonates; Cyanobacteria; Humanities and Social Sciences; Mass spectrometry; Mass spectroscopy; Microorganisms; multidisciplinary; Nitrogen; Nitrogen Fixation; Nitrogenation; Nutrients; Oxygenation; Pacific Ocean; Science; Science (multidisciplinary); Scientific imaging; Seawater - chemistry; Secondary ion mass spectrometry; Spectroscopy; Surface water; Water; Pacific Ocean
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-34585-y

Biological nitrogen fixation is a major important source of nitrogen for low-nutrient surface oceanic waters. Nitrogen-fixing (diazotrophic) cyanobacteria are believed to be the primary contributors to this process, but the contribution of non-cyanobacterial diazotrophic organisms in oxygenated surface water, while hypothesized to be important, has yet to be demonstrated. In this study, we used simultaneous 15 N-dinitrogen and 13 C-bicarbonate incubations combined with nanoscale secondary ion mass spectrometry analysis to screen tens of thousands of mostly particle-associated, cell-like regions of interest collected from the North Pacific Subtropical Gyre. These dual isotope incubations allow us to distinguish between non-cyanobacterial and cyanobacterial nitrogen-fixing microorganisms and to measure putative cell-specific nitrogen fixation rates. With this approach, we detect nitrogen fixation by putative non-cyanobacterial diazotrophs in the oxygenated surface ocean, which are associated with organic-rich particles (<210 µm size fraction) at two out of seven locations sampled. When present, up to 4.1% of the analyzed particles contain at least one active putative non-cyanobacterial diazotroph. The putative non-cyanobacterial diazotroph nitrogen fixation rates (0.76 ± 1.60 fmol N cell −1 d −1 ) suggest that these organisms are capable of fixing dinitrogen in oxygenated surface water, at least when attached to particles, and may contribute to oceanic nitrogen fixation. Nitrogen-fixing (diazotrophic) cyanobacteria provide a critical nutrient input to the ocean. Non-cyanobacterial diazotrophs are also thought to contribute, but they have not been observed to fix nitrogen. Using dual isotope labeling combined with nanoscale secondary ion mass spectrometry, this study demonstrates that putative non-cyanobacterial diazotrophs attached to particles can fix nitrogen.