A simple and precise method to detect sterol esterification activity of lecithin/cholesterol acyltransferase by high-performance liquid chromatography - Wang, Yu; Wang, Siming; Zhang, Lijiao; Zeng, Jie; Yang, Ruiyue; Li, Hongxia; Tang, Yueming; Chen, Wenxiang; Dong, Jun (Analytical and Bioanalytical Chemistry, 2018)
Keywords: 7-dehydrocholesterol; absorption; Acyltransferase; Adult; Aged; Analytical Chemistry; apolipoprotein A-I; Apolipoproteins; Biochemistry; blood serum; Cardiovascular diseases; Characterization and Evaluation of Materials; chemical bonding; Chemical properties; Chemical vapor deposition; Chemistry; Chemistry and Materials Science; Cholesterol; cholesterol acyltransferase; Chromatography; Chromatography, High Pressure Liquid; Coefficient of variation; Cofactors; correlation; Dehydrocholesterols; emulsifiers; Enzyme Assays; Esterification; Ethanol; Female; Food Science; Heart diseases; hexane; Hexanes; High density lipoprotein; high density lipoprotein cholesterol; High performance liquid chromatography; Humans; Laboratory Medicine; Lecithin; Linear functions; Lipid metabolism; Lipids; Liquid chromatography; Low density lipoprotein; Male; Metabolism; Methods; Middle Aged; Monitoring/Environmental Analysis; Observations; phosphatidylcholine-choline acyltransferase; Phosphatidylcholine-Sterol O-Acyltransferase; Phosphocholine; Reagents; Reproducibility; Reproducibility of Results; Research Paper; Risk assessment; Sterols; Substrate Specificity; Substrates; triacylglycerols; Triglycerides; Ultraviolet absorption; Young Adult; Lecithin: cholesterol acyltransferase; Sterol esterification; Cardiovascular disease; Enzyme activity; High-density lipoprotein; Chromatography