study of t-PA, u-PA and PAI-1 genes polymorphisms in patients with abdominal aortic aneurysm

• Published in: Molecular biology reports Vol. 41; no. 5; pp. 2859 - 2864
• Main Authors: Oszajca, Katarzyna, Wroński, Konrad, Janiszewska, Grażyna, Bieńkiewicz, Małgorzata, Bartkowiak, Jacek, Szemraj, Janusz
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.05.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Adult; Aged; Alleles; aneurysm; Aneurysms; Animal Anatomy; Animal Biochemistry; Aortic Aneurysm, Abdominal - genetics; Biomedical and Life Sciences; blood vessels; Case-Control Studies; extracellular matrix; Female; gelatinase A; Gene Frequency; genes; Genotype; Genotype & phenotype; Histology; Humans; INDEL Mutation; Life Sciences; Male; melting; Middle Aged; Morphology; pathogenesis; patients; plasmin; Plasminogen Activator Inhibitor 1 - genetics; polymerase chain reaction; Polymorphism; Polymorphism, Genetic; Polymorphism, Single Nucleotide; protective effect; proteins; risk; Risk factors; t-plasminogen activator; Tissue Plasminogen Activator - genetics; Urokinase-Type Plasminogen Activator - genetics; Veins & arteries; 675 4G/5G polymorphism; 7351 C/T polymorphism; Abdominal aortic aneurysm; 1788 C/T polymorphism; -; 844 G/A polymorphism; RFLP
• ISSN: 0301-4851; 1573-4978; 1573-4978
• DOI: 10.1007/s11033-014-3141-6

The most important feature of abdominal aortic aneurysm (AAA) pathogenesis is an enzymatic degradation of elastic lamellae and extracellular matrix proteins particularly with participation of matrix metalloproteinases. Plasmin, which is responsible for the dissolution of fibrin in blood vessels, plays also a key role in the cascade for activation of the metalloproteinases. The purpose of this study was to evaluate the influence of selected polymorphisms in genes coding for tissue plasminogen activator (−7351 C/T polymorphism), urokinase-type plasminogen activator (1788 C/T polymorphism) and plasminogen activator inhibitor 1 (−675 4G/5G and −844 G/A polymorphism) on the susceptibility to AAA. We performed a case–control study of 153 polish patients hospitalized due to AAA and compared them with matched healthy control subjects. The polymorphisms were ascertained through genotyping by polymerase chain reaction and restriction digestion of amplified fragments or through high-resolution melting analysis. In this study we have found lower frequency of wild-type GG genotype of the −844G/A PAI-1 polymorphism in cases than in controls, what may suggest the protective effect of this genotype for the risk of AAA development. None of the remaining polymorphisms tested were associated with AAA occurrence.