Systemic infection of Mycobacterium avium subspecies hominissuis and fungus in a pet dog

A 3-year-old neutered female poodle with a long history of dermatophytic skin disease was presented with lethargy, anorexia and progressive weight loss. Abdominal ultrasonography revealed markedly enlarged mesenteric lymph nodes and multiple hypoechoic foci in the spleen. Cytology of the mesenteric...

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Published inJournal of Veterinary Medical Science Vol. 78; no. 1; pp. 157 - 160
Main Authors KANG, WoonKi, KIM, Yongbaek, KIM, JaeMyung, KIM, Myung-Chul, JANG, Yunho
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.01.2016
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
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Online AccessGet full text
ISSN0916-7250
1347-7439
1347-7439
DOI10.1292/jvms.15-0285

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Abstract A 3-year-old neutered female poodle with a long history of dermatophytic skin disease was presented with lethargy, anorexia and progressive weight loss. Abdominal ultrasonography revealed markedly enlarged mesenteric lymph nodes and multiple hypoechoic foci in the spleen. Cytology of the mesenteric lymph nodes and spleen showed granulomatous inflammation with fungal organisms and negatively stained intracytoplasmic bacterial rods consistent with Mycobacteria spp. Based on culture, multiplex polymerase chain reaction and sequence analysis, the bacterium was identified as Mycobacterium avium subspecies hominissuis. Despite treatment with antibiotics, the dog’s condition deteriorated, and it died approximately 3 weeks after first presentation.
AbstractList A 3-year-old neutered female poodle with a long history of dermatophytic skin disease was presented with lethargy, anorexia and progressive weight loss. Abdominal ultrasonography revealed markedly enlarged mesenteric lymph nodes and multiple hypoechoic foci in the spleen. Cytology of the mesenteric lymph nodes and spleen showed granulomatous inflammation with fungal organisms and negatively stained intracytoplasmic bacterial rods consistent with Mycobacteria spp. Based on culture, multiplex polymerase chain reaction and sequence analysis, the bacterium was identified as Mycobacterium avium subspecies hominissuis. Despite treatment with antibiotics, the dog's condition deteriorated, and it died approximately 3 weeks after first presentation.
A 3-year-old neutered female poodle with a long history of dermatophytic skin disease was presented with lethargy, anorexia and progressive weight loss. Abdominal ultrasonography revealed markedly enlarged mesenteric lymph nodes and multiple hypoechoic foci in the spleen. Cytology of the mesenteric lymph nodes and spleen showed granulomatous inflammation with fungal organisms and negatively stained intracytoplasmic bacterial rods consistent with Mycobacteria spp. Based on culture, multiplex polymerase chain reaction and sequence analysis, the bacterium was identified as Mycobacterium avium subspecies hominissuis. Despite treatment with antibiotics, the dog's condition deteriorated, and it died approximately 3 weeks after first presentation.A 3-year-old neutered female poodle with a long history of dermatophytic skin disease was presented with lethargy, anorexia and progressive weight loss. Abdominal ultrasonography revealed markedly enlarged mesenteric lymph nodes and multiple hypoechoic foci in the spleen. Cytology of the mesenteric lymph nodes and spleen showed granulomatous inflammation with fungal organisms and negatively stained intracytoplasmic bacterial rods consistent with Mycobacteria spp. Based on culture, multiplex polymerase chain reaction and sequence analysis, the bacterium was identified as Mycobacterium avium subspecies hominissuis. Despite treatment with antibiotics, the dog's condition deteriorated, and it died approximately 3 weeks after first presentation.
A 3-year-old neutered female poodle with a long history of dermatophytic skin disease was presented with lethargy, anorexia and progressive weight loss. Abdominal ultrasonography revealed markedly enlarged mesenteric lymph nodes and multiple hypoechoic foci in the spleen. Cytology of the mesenteric lymph nodes and spleen showed granulomatous inflammation with fungal organisms and negatively stained intracytoplasmic bacterial rods consistent with Mycobacteria spp. Based on culture, multiplex polymerase chain reaction and sequence analysis, the bacterium was identified as Mycobacterium avium subspecies hominissuis. Despite treatment with antibiotics, the dog’s condition deteriorated, and it died approximately 3 weeks after first presentation.
Author KIM, Myung-Chul
KANG, WoonKi
KIM, JaeMyung
KIM, Yongbaek
JANG, Yunho
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– reference: 5. Gow, A. G. and Gow, D. J. 2008. Disseminated Mycobacterium avium complex infection in a dog. Vet. Rec. 162: 594–595.
– reference: 18. Thorel, M. F., Huchzermeyer, H. F. and Michel, A. L. 2001. Mycobacterium avium and Mycobacterium intracellulare infection in mammals. Rev. Sci. Tech. 20: 204–218.
– reference: 16. Shin, S. J., Lee, B. S., Koh, W.J., Manning, E. J., Anklam, K., Sreevatsan, S., Lambrecht, R. S. and Collins, M. T. 2010. Efficient differentiation of Mycobacterium avium complex species and subspecies by use of five-target multiplex PCR. J. Clin. Microbiol. 48: 4057–4062.
– reference: 3. Despierres, L., Cohen-Bacrie, S., Richet, H. and Drancourt, M. 2012. Diversity of Mycobacterium avium subsp. hominissuis mycobacteria causing lymphadenitis, France. Eur. J. Clin. Microbiol. Infect. Dis. 31: 1373–1379.
– reference: 9. Inderlied, C. B., Kemper, C. A. and Bermudez, L. E. 1993. The Mycobacterium avium complex. Clin. Microbiol. Rev. 6: 266–310.
– reference: 15. Rossi, M. C., Gori, A., Zehender, G., Marchetti, G., Ferrario, G., De Maddalena, C., Catozzi, L., Bandera, A., Degli Esposti, A. and Franzetti, F. 2000. A PCR-colorimetric microwell plate hybridization assay for detection of Mycobacterium tuberculosis and M. avium from culture samples and Ziehl-Neelsen-positive smears. J. Clin. Microbiol. 38: 1772–1776.
– reference: 6. Greene, C. and Gunn-Moore, D. 2006. Infections caused by slow-growing mycobacteria. pp. 462–477. In: Infectious Diseases of the Dog and Cat. 3th ed., Elsevier, St. Louis.
– reference: 8. Horn, B., Forshaw, D., Cousins, D. and Irwin, P. 2000. Disseminated Mycobacterium avium infection in a dog with chronic diarrhoea. Aust. Vet. J. 78: 320–325.
– reference: 2. Campora, L., Corazza, M., Zullino, C., Ebani, V. V. and Abramo, F. 2011. Mycobacterium avium subspecies hominissuis disseminated infection in a Basset Hound dog. J. Vet. Diagn. Invest. 23: 1083–1087.
– reference: 7. Haist, V., Seehusen, F., Moser, I., Hotzel, H., Deschl, U., Baumgärtner, W. and Wohlsein, P. 2008. Mycobacterium avium subsp. hominissuis infection in 2 pet dogs, Germany. Emerg. Infect. Dis. 14: 988–990.
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Snippet A 3-year-old neutered female poodle with a long history of dermatophytic skin disease was presented with lethargy, anorexia and progressive weight loss....
A 3-year-old neutered female poodle with a long history of dermatophytic skin disease was presented with lethargy, anorexia and progressive weight loss....
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StartPage 157
SubjectTerms Animals
Biopsy, Needle - veterinary
canine
Clinical Pathology
dermatophyte
Dog Diseases - diagnosis
Dog Diseases - diagnostic imaging
Dog Diseases - microbiology
Dog Diseases - pathology
Dogs
Female
fine needle aspiration
granuloma
Granuloma - veterinary
Lymph Nodes - diagnostic imaging
Lymph Nodes - microbiology
Lymph Nodes - pathology
Multiplex Polymerase Chain Reaction - veterinary
Mycobacterium avium
Mycobacterium avium Complex
Mycobacterium avium subspecies hominissuis
Mycobacterium avium-intracellulare Infection - diagnosis
Mycobacterium avium-intracellulare Infection - pathology
Mycobacterium avium-intracellulare Infection - veterinary
Spleen - diagnostic imaging
Spleen - microbiology
Spleen - pathology
Tuberculosis, Lymph Node - diagnosis
Tuberculosis, Lymph Node - pathology
Tuberculosis, Lymph Node - veterinary
Tuberculosis, Splenic - diagnosis
Tuberculosis, Splenic - pathology
Tuberculosis, Splenic - veterinary
Ultrasonography
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Title Systemic infection of Mycobacterium avium subspecies hominissuis and fungus in a pet dog
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