BDNF/TrkB通路活化星形胶质细胞对大鼠神经病理性痛的影响
目的观察外源性脑源性神经营养因子（BDNF）对大鼠机械痛阈和星形胶质细胞的影响,探讨BDNF参与疼痛调控的可能机制。方法将30只鞘内置管成功的大鼠随机分为空白对照组、安慰剂组、BDNF组、BDNF＋星形胶质细胞抑制剂组（BDNF＋氟代柠檬酸组）及BDNF＋酪氨酸激酶受体B（TrkB）抑制剂组（BDNF＋K252a组）,每组6只。予鞘内注入药物,1次/d×7 d。每次注药前1 h测定50%机械缩爪阈值（50%PWT）;末次给药后1 h取脊髓腰膨大,Western blotting法测定胶质细胞纤丝酸性蛋白（GFAP）及磷酸化TrkB的蛋白表达。结果 BDNF组大鼠后肢50%PWT较空白对照组显著...
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| Published in | 上海交通大学学报（医学版） Vol. 32; no. 3; pp. 279 - 282 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
上海交通大学附属第六人民医院疼痛科,上海200233
2012
上海交通大学附属第六人民医院麻醉科,上海200233%上海交通大学附属第六人民医院 疼痛科,上海,200233%上海交通大学附属第六人民医院 麻醉科,上海,200233 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-8115 |
| DOI | 10.3969/j.issn.1674-8115.2012.03.009 |
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| Abstract | 目的观察外源性脑源性神经营养因子（BDNF）对大鼠机械痛阈和星形胶质细胞的影响,探讨BDNF参与疼痛调控的可能机制。方法将30只鞘内置管成功的大鼠随机分为空白对照组、安慰剂组、BDNF组、BDNF＋星形胶质细胞抑制剂组（BDNF＋氟代柠檬酸组）及BDNF＋酪氨酸激酶受体B（TrkB）抑制剂组（BDNF＋K252a组）,每组6只。予鞘内注入药物,1次/d×7 d。每次注药前1 h测定50%机械缩爪阈值（50%PWT）;末次给药后1 h取脊髓腰膨大,Western blotting法测定胶质细胞纤丝酸性蛋白（GFAP）及磷酸化TrkB的蛋白表达。结果 BDNF组大鼠后肢50%PWT较空白对照组显著下降（P〈0.05）;给药后第7天,BDNF组大鼠脊髓腰膨大GFAP和磷酸化TrkB的蛋白表达较空白对照组显著升高（P〈0.01）。BDNF＋氟代柠檬酸组和BDNF＋K252a组50%PWT和GFAP蛋白表达水平与空白对照组比较,差异无统计学意义（P〉0.05）。BDNF＋K252a组磷酸化TrkB的蛋白表达与空白对照组比较,差异无统计学意义（P〉0.05）。结论 BDNF可能通过磷酸化TrkB受体使脊髓星形胶质细胞活化,进而参与神经病理性痛的发生和发展过程。 |
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| AbstractList | 目的观察外源性脑源性神经营养因子（BDNF）对大鼠机械痛阈和星形胶质细胞的影响,探讨BDNF参与疼痛调控的可能机制。方法将30只鞘内置管成功的大鼠随机分为空白对照组、安慰剂组、BDNF组、BDNF＋星形胶质细胞抑制剂组（BDNF＋氟代柠檬酸组）及BDNF＋酪氨酸激酶受体B（TrkB）抑制剂组（BDNF＋K252a组）,每组6只。予鞘内注入药物,1次/d×7 d。每次注药前1 h测定50%机械缩爪阈值（50%PWT）;末次给药后1 h取脊髓腰膨大,Western blotting法测定胶质细胞纤丝酸性蛋白（GFAP）及磷酸化TrkB的蛋白表达。结果 BDNF组大鼠后肢50%PWT较空白对照组显著下降（P〈0.05）;给药后第7天,BDNF组大鼠脊髓腰膨大GFAP和磷酸化TrkB的蛋白表达较空白对照组显著升高（P〈0.01）。BDNF＋氟代柠檬酸组和BDNF＋K252a组50%PWT和GFAP蛋白表达水平与空白对照组比较,差异无统计学意义（P〉0.05）。BDNF＋K252a组磷酸化TrkB的蛋白表达与空白对照组比较,差异无统计学意义（P〉0.05）。结论 BDNF可能通过磷酸化TrkB受体使脊髓星形胶质细胞活化,进而参与神经病理性痛的发生和发展过程。 R741.02; 目的 观察外源性脑源性神经营养因子(BDNF)对大鼠机械痛阈和星形胶质细胞的影响,探讨BDNF参与疼痛调控的可能机制.方法 将30只鞘内置管成功的大鼠随机分为空白对照组、安慰剂组、BDNF组、BDNF+星形胶质细胞抑制剂组(BDNF+氟代柠檬酸组)及BDNF+酪氨酸激酶受体B(TrkB)抑制剂组(BDNF+ K252a组),每组6只.予鞘内注入药物,1次/d×7 d.每次注药前1h测定50％机械缩爪阈值(50％ PWT);末次给药后1h取脊髓腰膨大,Western blotting法测定胶质细胞纤丝酸性蛋白(GFAP)及磷酸化TrkB的蛋白表达.结果 BDNF组大鼠后肢50％ PWT较空白对照组显著下降(P＜0.05);给药后第7天,BDNF组大鼠脊髓腰膨大GFAP和磷酸化TrkB的蛋白表达较空白对照组显著升高(P＜0.01).BDNF+氟代柠檬酸组和BDNF+K252a组50％ PWT和GFAP蛋白表达水平与空白对照组比较,差异无统计学意义(P＞0.05).BDNF+ K252a组磷酸化TrkB的蛋白表达与空白对照组比较,差异无统计学意义(P＞0.05).结论 BDNF可能通过磷酸化TrkB受体使脊髓星形胶质细胞活化,进而参与神经病理性痛的发生和发展过程. |
| Author | 汪静 张昕 江伟 杜冬萍 |
| AuthorAffiliation | 上海交通大学附属第六人民医院疼痛科,上海200233 上海交通大学附属第六人民医院麻醉科,上海200233 |
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| Author_FL | DU Dong-ping ZHANG Xin JIANG Wei WANG Jing |
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| DocumentTitleAlternate | Contribution of BDNF/TrkB pathway to development of neuropathic pain by activation of astrocytes in rats |
| DocumentTitle_FL | Contribution of BDNF/TrkB pathway to development of neuropathic pain by activation of astrocytes in rats |
| EndPage | 282 |
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| Keywords | 脑源性神经营养因子 磷酸化酪氨酸激酶受体B K252a 胶质细胞纤丝酸性蛋白 氟代柠檬酸 神经病理性痛 |
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| Notes | WANG Jing,ZHANG Xin,JIANG Wei,DU Dong-ping（1.Pain Management Center,2.Department of Anesthesiology,the Sixth People＇s Hospital,Shanghai Jiaotong University,Shanghai 200233,China） Objective To investigate the effects of exogenous brain-derived neurotrophic factor（BDNF） on mechanical pain threshold and astrocytes,and explore the potential mechanism of BDNF-induced pain.Methods Thirty rats with successful intrathecal catheterization were randomly divided into blank control group,placebo group,BDNF group,BDNF＋astrocyte inhibitor group（BDNF＋fluorocitrate group） and BDNF＋tyrosine kinase receptor B（TrkB） inhibitor group（BDNF＋K252a group）,with 6 rats in each group.Intrathecal administration was performed once daily for 7 d.Fifty percent paw withdrawal threshold（50% PWT） was measured 1 h before each injection.Spinal enlargement parts were obtained 1 h after the last administration,and the expression of glial fibrillary acidic protein（GFAP） and phosphorylated TrkB protein was detected by Western blotting.Results Compare |
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| Publisher | 上海交通大学附属第六人民医院疼痛科,上海200233 上海交通大学附属第六人民医院麻醉科,上海200233%上海交通大学附属第六人民医院 疼痛科,上海,200233%上海交通大学附属第六人民医院 麻醉科,上海,200233 |
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| Snippet | 目的观察外源性脑源性神经营养因子（BDNF）对大鼠机械痛阈和星形胶质细胞的影响,探讨BDNF参与疼痛调控的可能机制。方法将30只鞘内置管成功的大鼠随机分为空白对照组、安慰剂... R741.02; 目的 观察外源性脑源性神经营养因子(BDNF)对大鼠机械痛阈和星形胶质细胞的影响,探讨BDNF参与疼痛调控的可能机制.方法 将30只鞘内置管成功的大鼠随机分为空白对照... |
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| SubjectTerms | K252a 氟代柠檬酸 磷酸化酪氨酸激酶受体B 神经病理性痛 胶质细胞纤丝酸性蛋白 脑源性神经营养因子 |
| Title | BDNF/TrkB通路活化星形胶质细胞对大鼠神经病理性痛的影响 |
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