七龙天对低氧性肺动脉高压大鼠IL-1β、TNF-α的影响

目的观察七龙天对低氧性肺动脉高压（HPH）大鼠白细胞介素-1β（IL-1β）、肿瘤坏死因-α（TNF-α）的影响。方法将雄性SD大鼠随机分为6组：正常组、模型组、波生坦组、七龙天低剂量组、七龙天中剂量组、七龙天高剂量组。正常组大鼠置于正常环境中饲养,除正常组外所有实验大鼠均采用常压低氧法制模,从制模第1天始给药,连续3周。在直视下行右心导管术测定肺血流动力学参数,以RT-PCR法检测肺组织中IL-1β、TNF-α的mRNA表达及免疫组织化学技术检测IL-1β、TNF-α在肺小动脉内膜中的蛋白表达。结果七龙天及波生坦组均可明显降低大鼠肺动脉高压,且以七龙天高剂量组效果为佳,疗效呈药物剂量依赖表现...

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Published in北京中医药大学学报 Vol. 39; no. 11; pp. 915 - 919
Main Author 冷萍 余晓玲 魏丹霞 苏婷 付义
Format Journal Article
LanguageChinese
Published 云南中医学院 云南 650500%云南中医学院第三附属医院 2016
Subjects
七龙天
大鼠
白细胞介素-1β
肺动脉高压
肿瘤坏死因子-α
Qilongtian
大鼠
hypoxic pulmonary hypertension
tumor necrosis factor-α
肺动脉高压
七龙天
rats
白细胞介素-1β
肿瘤坏死因子-α
interleukin-1β
Online AccessGet full text
ISSN1006-2157
DOI10.3969/j.issn.1006-2157.2016.11.006

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Summary:目的观察七龙天对低氧性肺动脉高压（HPH）大鼠白细胞介素-1β（IL-1β）、肿瘤坏死因-α（TNF-α）的影响。方法将雄性SD大鼠随机分为6组：正常组、模型组、波生坦组、七龙天低剂量组、七龙天中剂量组、七龙天高剂量组。正常组大鼠置于正常环境中饲养,除正常组外所有实验大鼠均采用常压低氧法制模,从制模第1天始给药,连续3周。在直视下行右心导管术测定肺血流动力学参数,以RT-PCR法检测肺组织中IL-1β、TNF-α的mRNA表达及免疫组织化学技术检测IL-1β、TNF-α在肺小动脉内膜中的蛋白表达。结果七龙天及波生坦组均可明显降低大鼠肺动脉高压,且以七龙天高剂量组效果为佳,疗效呈药物剂量依赖表现。与正常组比较,各组的IL-1β、TNF-α表达水平（包括mRNA及蛋白）均有所增加,尤以模型组、波生坦组呈非常显著性差异（P〈0.01）;与模型组比较,波生坦组无显著性差异（P〉0.05）,七龙天各组可明显下调IL-1β、TNF-α表达水平（P〈0.01或P〈0.05）,以七龙天高、中剂量组更为显著（P〈0.01或P〈0.05）。结论不同剂量七龙天均可降低肺动脉高压,且高剂量七龙天疗效优于波生坦。其降压机制与降低IL-1β及TNF-α表达量有关,但仍可能受其他血管张力因子及血管新生因子影响。
Bibliography:11-3574/R
LENG Ping1 , YU Xiaoling2, WEI Danxia2, SU TingI , FU Yi2 （1 Yunnan University of Traditional Chinese Medicine, Yunnan 650500; 2 The Third Affiliated Hospital of Yunnan University of Traditional Chinese Medicine）
Qilongtian; hypoxic pulmonary hypertension; interleukin-1β; tumor necrosis factor-α; rats
Objective To study the effects of Qilongtian on interleukin-1β（ IL-1β） and tumor necrosis factor-α（ TNF-α） in hypoxic pulmonary hypertension（ HPH） rats. Methods All male rats were randomly divided into six groups： normal group,model group,bosentan group,Qilongtian high-,mid- and low- group. Except the rats in normal group,those in other five groups were under atmospheric hypoxia to establish HPH model. From the first day of modeling,all medication groups were i. g corresponding drugs for consecutive three weeks. Pulmonary hemodynamic parameters were detected by right heart catheterization under direct vision,mRNA expression of IL-1β and TNF-α in lung tissue were measured using ELISA,protein expression of
ISSN:1006-2157
DOI:10.3969/j.issn.1006-2157.2016.11.006