杆状病毒载体介导钠碘转运体基因放射治疗肝癌的实验研究
目的探讨以杆状病毒为载体、钠碘转运体（NIS）基因介导131I放射治疗肝细胞癌的可行性。方法通过Bac-to-Bac杆状病毒表达系统制备重组的绿色荧光蛋白（GFP）和NIS基因杆状病毒（Bac-GFP和Bac-NIS）。采用流式细胞仪检测在有无丁酸钠条件下HepG2细胞的感染效率和荧光表达强度,以及在高感染复数（MOI）杆状病毒条件下对HepG2细胞的毒性。进行Bac-NIS感染HepG2细胞的动态摄碘实验和高氯酸钠（NaClO4）摄碘抑制实验,以及Bac-NIS感染HepG2细胞的131I杀伤细胞克隆形成实验。结果 HepG2的感染效率和荧光强度随着Bac-GFP MOI的增加而增加,Bac...
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| Published in | 上海交通大学学报（医学版） Vol. 32; no. 5; pp. 560 - 566 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
上海交通大学医学院附属瑞金医院核医学科,上海,200025
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-8115 |
| DOI | 10.3969/j.issn.1674-8115.2012.05.006 |
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| Abstract | 目的探讨以杆状病毒为载体、钠碘转运体（NIS）基因介导131I放射治疗肝细胞癌的可行性。方法通过Bac-to-Bac杆状病毒表达系统制备重组的绿色荧光蛋白（GFP）和NIS基因杆状病毒（Bac-GFP和Bac-NIS）。采用流式细胞仪检测在有无丁酸钠条件下HepG2细胞的感染效率和荧光表达强度,以及在高感染复数（MOI）杆状病毒条件下对HepG2细胞的毒性。进行Bac-NIS感染HepG2细胞的动态摄碘实验和高氯酸钠（NaClO4）摄碘抑制实验,以及Bac-NIS感染HepG2细胞的131I杀伤细胞克隆形成实验。结果 HepG2的感染效率和荧光强度随着Bac-GFP MOI的增加而增加,Bac-GFP对HepG2具有较高的感染效率（MOI=200时,感染效率为65%）,丁酸钠可进一步提高HepG2的感染效率。在高MOI（MOI=400）条件下,未见Bac-GFP对HepG2的细胞毒性作用,与对照组（MOI=0）比较差异无统计学意义（P〉0.05）。Bac-NIS感染的HepG2细胞表现出摄碘功能和NaClO4抑制的特性。细胞克隆形成实验表明,Bac-NIS感染的HepG2细胞克隆存活率明显低于Bac-GFP感染的HepG2细胞,差异有统计学意义（P〈0.01）。结论 Bac-NIS可有效介导肝癌细胞的碘摄取,高效杀伤肿瘤细胞,为肝癌细胞的靶向基因放射治疗提供了实验基础。 |
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| AbstractList | R73-36; 目的 探讨以杆状病毒为载体、钠碘转运体(NIS)基因介导131I放射治疗肝细胞癌的可行性.方法 通过Bac-to-Bac杆状病毒表达系统制备重组的绿色荧光蛋白(GFP)和NIS基因杆状病毒(Bac-GFP和Bac-NIS).采用流式细胞仪检测在有无丁酸钠条件下HepG2细胞的感染效率和荧光表达强度,以及在高感染复数(Mol)杆状病毒条件下对HepG2细胞的毒性.进行Bac-NIS 感染HepG2细胞的动态摄碘实验和高氯酸钠(NaClO4)摄碘抑制实验,以及Bac-NIS感染HepG2细胞的131I杀伤细胞克隆形成实验.结果HepG2的感染效率和荧光强度随着Bac-GFP MOI的增加而增加,Bac-GFP对HepG2具有较高的感染效率(MOI=200时,感染效率为65％),丁酸钠可进一步提高HepG2的感染效率.在高MOI(MOI=400)条件下,未见Bac-GFP对HepG2的细胞毒性作用,与对照组(MOI=0)比较差异无统计学意义(P＞0.05).Bac-NIS感染的HepG2细胞表现出摄碘功能和NaCIO4抑制的特性.细胞克隆形成实验表明,Bac-NIS感染的HepG2细胞克隆存活率明显低于Bac-GFP感染的HepG2细胞,差异有统计学意义(P＜0.01).结论 Bac-NIS可有效介导肝癌细胞的碘摄取,高效杀伤肿瘤细胞,为肝癌细胞的靶向基因放射治疗提供了实验基础. 目的探讨以杆状病毒为载体、钠碘转运体（NIS）基因介导131I放射治疗肝细胞癌的可行性。方法通过Bac-to-Bac杆状病毒表达系统制备重组的绿色荧光蛋白（GFP）和NIS基因杆状病毒（Bac-GFP和Bac-NIS）。采用流式细胞仪检测在有无丁酸钠条件下HepG2细胞的感染效率和荧光表达强度,以及在高感染复数（MOI）杆状病毒条件下对HepG2细胞的毒性。进行Bac-NIS感染HepG2细胞的动态摄碘实验和高氯酸钠（NaClO4）摄碘抑制实验,以及Bac-NIS感染HepG2细胞的131I杀伤细胞克隆形成实验。结果 HepG2的感染效率和荧光强度随着Bac-GFP MOI的增加而增加,Bac-GFP对HepG2具有较高的感染效率（MOI=200时,感染效率为65%）,丁酸钠可进一步提高HepG2的感染效率。在高MOI（MOI=400）条件下,未见Bac-GFP对HepG2的细胞毒性作用,与对照组（MOI=0）比较差异无统计学意义（P〉0.05）。Bac-NIS感染的HepG2细胞表现出摄碘功能和NaClO4抑制的特性。细胞克隆形成实验表明,Bac-NIS感染的HepG2细胞克隆存活率明显低于Bac-GFP感染的HepG2细胞,差异有统计学意义（P〈0.01）。结论 Bac-NIS可有效介导肝癌细胞的碘摄取,高效杀伤肿瘤细胞,为肝癌细胞的靶向基因放射治疗提供了实验基础。 |
| Author | 乌海飞 刘帅 潘昱 周翔 尹红燕 李彪 江旭峰 张一帆 |
| AuthorAffiliation | 上海交通大学医学院附属瑞金医院核医学科,上海200025 |
| AuthorAffiliation_xml | – name: 上海交通大学医学院附属瑞金医院核医学科,上海,200025 |
| Author_FL | WU Hai-fei PAN Yu LI Biao LIU Shuai ZHANG Yi-fan JIANG Xu-feng YIN Hong-yan ZHOU Xiang |
| Author_FL_xml | – sequence: 1 fullname: WU Hai-fei – sequence: 2 fullname: LIU Shuai – sequence: 3 fullname: PAN Yu – sequence: 4 fullname: ZHOU Xiang – sequence: 5 fullname: YIN Hong-yan – sequence: 6 fullname: LI Biao – sequence: 7 fullname: JIANG Xu-feng – sequence: 8 fullname: ZHANG Yi-fan |
| Author_xml | – sequence: 1 fullname: 乌海飞 刘帅 潘昱 周翔 尹红燕 李彪 江旭峰 张一帆 |
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| ClassificationCodes | R73-36 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.3969/j.issn.1674-8115.2012.05.006 |
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| DocumentTitleAlternate | Experiment research on baculovirus-mediated sodium-iodine symporter gene radiotherapy for liver cancer |
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| Issue | 5 |
| Keywords | 杆状病毒 基因治疗 肝细胞癌 钠碘同向转运体 放射治疗 |
| Language | Chinese |
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| Notes | Objective To investigate the feasibility of baculovirus-mediated 131I radiotherapy by carrying sodium iodide symporter（NIS） gene for hepatocellular carcinoma.Methods The recombinant baculoviruses carrying green fluorescent protein（GFP）or NIS gene（Bac-GFP and Bac-NIS） were prepared by Bac-to-Bac baculovirus expression system.The infection efficiency and fluorescence intensity of HepG2 cells were determined by flow cytometry in the presence or absence of sodium butyrate,and the cytotoxic effect of baculovirus on HepG2 cells was also determined in the condition of high multiplicity of infection（MOI）.Furthermore,HepG2 cells infected with Bac-NIS were examined with dynamic iodide uptake test and natrium perchloricum（NaClO4） iodide uptake inhibition test,and cell clone formation assay was adopted to evaluate the killing efficacy of 131I in HepG2 cells infected with Bac-NIS.Results The infection efficiency and fluorescence intensity of HepG2 cells increased along with the increase of Bac-GFP MOI.The infection effici |
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| PublicationTitle | 上海交通大学学报（医学版） |
| PublicationTitleAlternate | Journal of Shanghai Jiaotong University：Medical Science |
| PublicationTitle_FL | Journal of Shanghai Jiaotong University(Medical Science) |
| PublicationYear | 2012 |
| Publisher | 上海交通大学医学院附属瑞金医院核医学科,上海,200025 |
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| Snippet | 目的探讨以杆状病毒为载体、钠碘转运体（NIS）基因介导131I放射治疗肝细胞癌的可行性。方法通过Bac-to-Bac杆状病毒表达系统制备重组的绿色荧光蛋白（GFP）和NIS基因杆状病... R73-36; 目的 探讨以杆状病毒为载体、钠碘转运体(NIS)基因介导131I放射治疗肝细胞癌的可行性.方法 通过Bac-to-Bac杆状病毒表达系统制备重组的绿色荧光蛋白(GFP)和NIS基因杆... |
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| SubjectTerms | 基因治疗 放射治疗 杆状病毒 肝细胞癌 钠碘同向转运体 |
| Title | 杆状病毒载体介导钠碘转运体基因放射治疗肝癌的实验研究 |
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