百草枯中毒小鼠肺组织转化生长因子β和缺氧诱导因子1α的表达

日的探讨百草枯中毒小鼠肺组织转化生长因子β（TGF—β）和缺氧诱导因子1d（HIF-1α）的表达变化。方法将30只雄性C57BL／6J小鼠随机分为对照组（n=10）和百草枯组（n=20）。百草枯组腹腔注射百草枯20mg／kg，对照组腹腔注射等体积生理盐水。在染毒后第7天处死10只百草枯组小鼠，第28天处死其余小鼠。通过苏木精一伊红（HE）染色和Masson染色进行肺组织病理学观察；免疫组织化学法检测肺组织TGF—B和HIF-1α蛋白的表达。采用Pearson相关分析法分析TGF—β蛋白与HIF-1α蛋白表达的相关性。结果HE染色和Masson染色结果均显示：在染毒后第7天，百草枯组可见肺纤维化...

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Published in上海交通大学学报（医学版） Vol. 32; no. 12; pp. 1594 - 1599
Main Author 杭瑛 钱洁 朱长清 吕利雄 陈怡
Format Journal Article
LanguageChinese
Published 上海交通大学医学院附属仁济医院急诊内科,上海,200001 2012
Subjects
中毒
百草枯
肺纤维化
转化生长因子β缺氧诱导因子1α
转化生长因子β
百草枯
肺纤维化
中毒
缺氧诱导因子1α
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ISSN1674-8115
DOI10.3969/j.issn.1674-8115.2012.12.016

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Summary:日的探讨百草枯中毒小鼠肺组织转化生长因子β（TGF—β）和缺氧诱导因子1d（HIF-1α）的表达变化。方法将30只雄性C57BL／6J小鼠随机分为对照组（n=10）和百草枯组（n=20）。百草枯组腹腔注射百草枯20mg／kg，对照组腹腔注射等体积生理盐水。在染毒后第7天处死10只百草枯组小鼠，第28天处死其余小鼠。通过苏木精一伊红（HE）染色和Masson染色进行肺组织病理学观察；免疫组织化学法检测肺组织TGF—B和HIF-1α蛋白的表达。采用Pearson相关分析法分析TGF—β蛋白与HIF-1α蛋白表达的相关性。结果HE染色和Masson染色结果均显示：在染毒后第7天，百草枯组可见肺纤维化表现；染毒后第28天，肺纤维化程度加重。染毒后第7天百草枯组小鼠肺组织TGF-p蛋白的表达明显高于对照组，染毒后第28天TGF—β蛋白的表达高于染毒后第7天，差异均有统计学意义（P〈0．05）。染毒后第7天和第28天，百草枯组小鼠肺组织HIF-1d蛋白的表达均明显高于对照组（P〈0．05）；染毒后第28天的HIF-1d蛋白的表达高于第7天，但差异无统计学意义（P〉0．05）。在染毒后第7天和第28天，百草枯组TGF—B蛋白与HIF-1β蛋白的表达均无相关性（r=0．295，P=0．630；r=0．218，P=0．725）。结论百草枯中毒可上调小鼠肺组织的TGF—β和HIF-1仅蛋白，TGF—p和HIF-1β可能通过不同途径参与了肺纤维化的形成。
Bibliography:Objective To investigate the expression of transforming growth factor-β （TGF-β） and hypoxia inducible factor- lc~ （HIF-lct） in mice with paraquat poisoning. Methods Thirty male C57BL/6J mice were randomly divided into control group （n = 10） and paraquat group （n =20）. Paraquat （20 mg/kg） was intraperitoneally administered to mice in paraquat group, while the same volume of normal saline was intraperitoneally administered to those in control group. Ten mice in paraquat group were sacrificed on the 7th day after treatment with paraquat, and the rest of mice were killed on the 28th day. The pathological examinations of lung tissues were performed with HE staining and Masson staining, and the expression of TGF-β and HIF-1 α protein in lung tissues was detected by immunohistochemistry. The correlation of expression of TGF-β protein with expression of HIF-lα protein was explored by Pearson correlation analysis. Results HE staining and Masson staining indicated that there were presentations of lung fibrosis in paraq
ISSN:1674-8115
DOI:10.3969/j.issn.1674-8115.2012.12.016