Highly Specific Detection of Five Exotic Quarantine Plant Viruses using RT-PCR

• Published in: The plant pathology journal Vol. 29; no. 1; pp. 99 - 104
• Main Authors: Choi, H.S., Seoul National University, Seoul, Republic of Korea, Cho, W.K., Seoul National University, Seoul, Republic of Korea, Yu, J.S., Seoul National University, Seoul, Republic of Korea, Lee, J.S., Seoul National University, Seoul, Republic of Korea, Kim, K.H., Seoul National University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국식물병리학회 01.03.2013; Korean Society of Plant Pathology; Hanrimwon Publishing Company
• Subjects: Beet black scorch virus; Beet black scorch virus,Beet necrotic yellow vein virus,Eggplant mottled dwarf virus,zonate spot virus,Rice yellow mottle virus; Beet necrotic yellow vein virus; Eggplant mottled dwarf virus; Pelargonium zonate spot virus; RHIZOMANIA VIRUS; RICE YELLOW MOTTLE SOBEMOVIRUS; Rice yellow mottle virus; SOBEMOVIRUS MARBRURE JAUNE RIZ; SOBEMOVIRUS MOTEADO AMARILLO ARROZ; VIRUS DE LA RHIZOMANIE; VIRUS DE LA RIZOMANIA; 농학; Rice yellow mottle virus; Pelargonium zonate spot virus; Beet black scorch virus; Beet necrotic yellow vein virus; Eggplant mottled dwarf virus
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/ppj.nt.09.2012.0142

To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amplified the targets, and gradient PCRs indicated that the optimal temperature for the 14 useful primer sets was 51.9 oC. Some primer sets worked well regardless of annealing temperature while others required a very specific annealing temperature. A primer specificity test using plant total RNAs and cDNAs of other plant virusinfected samples demonstrated that the designed primer sets were highly specific and generated reproducible results. The newly developed RT-PCR primer sets would be useful for quarantine inspections aimed at preventing the entry of exotic plant viruses into Korea.