Benchmarking MicrobIEM – a user-friendly tool for decontamination of microbiome sequencing data - Hülpüsch, Claudia; Rauer, Luise; Nussbaumer, Thomas; Schwierzeck, Vera; Bhattacharyya, Madhumita; Erhart, Veronika; Traidl-Hoffmann, Claudia; Reiger, Matthias; Neumann, Avidan U. (BMC biology, 2023)
Keywords: 16S rRNA gene sequencing; Algorithms; Bacteria - genetics; Benchmarking; Benchmarks; Bioinformatic decontamination; Biomass; Biomedical and Life Sciences; Composition; Contaminants; Data analysis; Datasets; Decontam; Decontamination; Electronic data processing; Gene sequencing; Genera; Graphical user interface; High-Throughput Nucleotide Sequencing - methods; Humans; Life Sciences; Low-biomass microbiome; Methods; Microbiological research; Microbiomes; Microbiota (Symbiotic organisms); Microbiota - genetics; Microorganisms; Negative control; Parameters; Pollutant removal; Quality control; Research Article; RNA; RNA sequencing; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA - methods; Skin; Standard components; Statistical software; Youden’s index; Germany; Youden’s index; Negative control; Bioinformatic decontamination; Low-biomass microbiome; 16S rRNA gene sequencing; Decontam; SourceTracker