异补骨脂素对去卵巢骨质疏松小鼠骨髓间充质干细胞作用机制研究

目的探讨异补骨脂素对去卵巢骨质疏松小鼠骨髓间充质干细胞（bone mesenehymal stem cells,BMSCs）作用机制。方法18只C57/BL6雌鼠随机平均分为假手术组（Sham）、去卵巢组（OVX）和去卵巢加异补骨脂素组（OVX＋ISO）,各组6只。OVX组和OVX＋ISO组分离背部近髂脊处肌肉,取出双侧卵巢,结扎上部输卵管,剪去双侧输卵管。Sham组相同入路后剪去包裹卵巢周围的部分脂肪组织。OVX＋ISO组在去卵巢前5天开始灌胃异补骨脂素,灌胃剂量为20 mg/（kg·d）,灌胃持续时间2个月,Sham组以等剂量生理盐水灌胃。造模后12周处死小鼠,取股骨下段评价骨髓腔中脂肪细...

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Published in中国骨质疏松杂志 Vol. 22; no. 8; pp. 980 - 984
Main Author 王剑 陈天宇 王钢 格日勒图
Format Journal Article
LanguageChinese
Published 内蒙古自治区人民医院创伤骨科,呼和浩特,010010%南方医科大学第三附属医院,广州,510630%南方医科大学南方医院,广州,510515 2016
Subjects
小鼠
异补骨脂素
骨质疏松
骨髓间充质干细胞
Isopsoralen
骨质疏松
Osteoporosis
异补骨脂素
骨髓间充质干细胞
Bone mesenchymal stem cells
小鼠
Mice
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ISSN1006-7108
DOI10.3969/j.issn.1006-7108.2016.08.009

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Summary:目的探讨异补骨脂素对去卵巢骨质疏松小鼠骨髓间充质干细胞（bone mesenehymal stem cells,BMSCs）作用机制。方法18只C57/BL6雌鼠随机平均分为假手术组（Sham）、去卵巢组（OVX）和去卵巢加异补骨脂素组（OVX＋ISO）,各组6只。OVX组和OVX＋ISO组分离背部近髂脊处肌肉,取出双侧卵巢,结扎上部输卵管,剪去双侧输卵管。Sham组相同入路后剪去包裹卵巢周围的部分脂肪组织。OVX＋ISO组在去卵巢前5天开始灌胃异补骨脂素,灌胃剂量为20 mg/（kg·d）,灌胃持续时间2个月,Sham组以等剂量生理盐水灌胃。造模后12周处死小鼠,取股骨下段评价骨髓腔中脂肪细胞量,行micro-CT扫描评价股骨下段骨量改变及RUNX2、PPAR-γ免疫荧光检测。结果异补骨脂素能明显减少由于去卵巢引起的股骨下段脂肪细胞增多;与此同时,异补骨脂素治疗能明显改善由于去卵巢引起的股骨下段骨量丢失,即OVX＋ISO组骨小梁厚度（Tb.Th）、骨体积/总体积（BV/TV）、骨小梁数量（Tb.N）大于OVX组,差异具有统计学意义（P〈0.05）;而OVX＋ISO组骨小梁体积（Tb.Sp）小于OVX组,差异具有统计学意义（P〈0.05）。对去卵巢C57/BL6小鼠股骨下段进行RUNX2和PPAR-γ免疫荧光提示,异补骨脂素治疗能增加股骨下段由于去卵巢引起的RUNX2表达降低,同时能抑制PPAR-γ表达增加,即OVX＋ISO组股骨下段RUNX2、PPAR-γ免疫荧光表达强度高于OVX组,差异具有统计学意义（P〈0.05）。结论异补骨质素对去卵巢小鼠BMSCs的作用机制为治疗骨质疏松提供了新的临床治疗手段。
Bibliography:Isopsoralen; Bone mesenchymal stem cells; Osteoporosis; Mice
Objective To study the effects of isopsoralen on bone marrowmesenchymal stem cells（ BMSCs） of mice with ovariectomized osteoporosis and to explore the relevant mechanisms. Methods Eighteen C57 / BL6 female rats were randomly divided into sham operation group（ sham）,ovariectomized group（ OVX） and ovariectomized plus isopsoralen group（ OVX ＋ISO）,with 6 rats in each group. In the OVX group and OVX ＋ ISO group,surgery was performed from the back of the proximal iliac spine muscle,and bilateral ovaries were removed and the bilateral fallopian tubes cut off after the ligation of the upper fallopian tube. In the Sham group,part of the fat tissue around the ovary was removed after the same procedure. All rats of the OVX ＋ ISO group was gavaged isopsoralen at a dose of 20 mg /（ kg·d） for two months began 5 days before the removal of ovaries. All of the rats in the sham group were gavaged with equal dose of normal saline. After 12 weeks,rats were sacrificed to
ISSN:1006-7108
DOI:10.3969/j.issn.1006-7108.2016.08.009