改良Blocker PCR检测EGFR-TKI耐药后非小细胞肺癌血浆EGFR T790M突变的价值
目的探讨改良PCR（Blocker PCR）方法在晚期非小细胞肺癌（non-small-cell lung cancer,NSCLC）患者血浆游离DNA（cell free DNA,cfDNA）中检测继发EGFR T790 M突变的应用价值。方法采用Blocker PCR方法对127例表皮生长因子受体酪氨酸激酶抑制剂（epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI）耐药后肺癌患者的血浆标本行EGFR敏感突变和T790 M突变检测,统计T790 M突变的检出率;为验证Blocker PCR血浆检测的可靠性,部...
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| Published in | 复旦学报（医学版） Vol. 45; no. 1; pp. 45 - 51 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
复旦大学附属中山医院呼吸内科 上海200032%上海允英医疗科技有限公司 上海201216
2018
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-8467 |
| DOI | 10.3969/j.issn.1672-8467.2018.01.007 |
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| Abstract | 目的探讨改良PCR（Blocker PCR）方法在晚期非小细胞肺癌（non-small-cell lung cancer,NSCLC）患者血浆游离DNA（cell free DNA,cfDNA）中检测继发EGFR T790 M突变的应用价值。方法采用Blocker PCR方法对127例表皮生长因子受体酪氨酸激酶抑制剂（epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI）耐药后肺癌患者的血浆标本行EGFR敏感突变和T790 M突变检测,统计T790 M突变的检出率;为验证Blocker PCR血浆检测的可靠性,部分患者血浆标本行二代测序（next generation sequencing,NGS）和Blocker PCR配对比较;获得配对血浆和组织的病例行Blocker PCR配对检测。结果在127例采用Blocker PCR方法检测的EGFR-TKI耐药NSCLC患者中,T790 M耐药突变的检出率为40.15%（51/127）,其中21.56%（11/51）为单纯T790 M耐药突变,78.44%（40/51）为T790 M合并原有EGFR敏感突变。组织与血浆配对检测中,EGFR-TKI耐药后二次活检组织标本T790 M的检出率为54.54%（6/11）,血浆T790 M的检出率为43.75%（14/32）。在同时行Blocker PCR和二代测序基因检测的18例患者中,敏感突变位点及T790 M突变位点在两种检测方法中的一致率均为100%。结论在EGFR-TKI耐药后的NSCLC患者中使用Blocker PCR检测血浆T790 M突变是对组织活检的重要补充。 |
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| AbstractList | R563.9%R734.2; 目的 探讨改良PCR(Blocker PCR)方法在晚期非小细胞肺癌(non-small-cell lung cancer,NSCLC)患者血浆游离DNA(cell free DNA,cfDNA)中检测继发EGFR T790M突变的应用价值.方法 采用Blocker PCR方法对127例表皮生长因子受体酪氨酸激酶抑制剂(epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI)耐药后肺癌患者的血浆标本行EGFR敏感突变和T790M突变检测,统计T790M突变的检出率;为验证Blocker PCR血浆检测的可靠性,部分患者血浆标本行二代测序(next generation sequencing,NGS)和Blocker PCR配对比较;获得配对血浆和组织的病例行Blocker PCR配对检测.结果 在127例采用BlockerPCR方法检测的EGFR-TKI耐药NSCLC患者中,T790M耐药突变的检出率为40.15％ (51/127),其中21.56％ (11/51)为单纯T790M耐药突变,78.44％ (40/51)为T790M合并原有EGFR敏感突变.组织与血浆配对检测中,EGFR-TKI耐药后二次活检组织标本T790M的检出率为54.54％(6/11),血浆T790M的检出率为43.75％ (14/32).在同时行Blocker PCR和二代测序基因检测的18例患者中,敏感突变位点及T790M突变位点在两种检测方法中的一致率均为100％.结论 在EGFR-TKI耐药后的NSCLC患者中使用Blocker PCR检测血浆T790M突变是对组织活检的重要补充. 目的探讨改良PCR（Blocker PCR）方法在晚期非小细胞肺癌（non-small-cell lung cancer,NSCLC）患者血浆游离DNA（cell free DNA,cfDNA）中检测继发EGFR T790 M突变的应用价值。方法采用Blocker PCR方法对127例表皮生长因子受体酪氨酸激酶抑制剂（epidermal growth factor receptor-tyrosine kinase inhibitor,EGFR-TKI）耐药后肺癌患者的血浆标本行EGFR敏感突变和T790 M突变检测,统计T790 M突变的检出率;为验证Blocker PCR血浆检测的可靠性,部分患者血浆标本行二代测序（next generation sequencing,NGS）和Blocker PCR配对比较;获得配对血浆和组织的病例行Blocker PCR配对检测。结果在127例采用Blocker PCR方法检测的EGFR-TKI耐药NSCLC患者中,T790 M耐药突变的检出率为40.15%（51/127）,其中21.56%（11/51）为单纯T790 M耐药突变,78.44%（40/51）为T790 M合并原有EGFR敏感突变。组织与血浆配对检测中,EGFR-TKI耐药后二次活检组织标本T790 M的检出率为54.54%（6/11）,血浆T790 M的检出率为43.75%（14/32）。在同时行Blocker PCR和二代测序基因检测的18例患者中,敏感突变位点及T790 M突变位点在两种检测方法中的一致率均为100%。结论在EGFR-TKI耐药后的NSCLC患者中使用Blocker PCR检测血浆T790 M突变是对组织活检的重要补充。 |
| Abstract_FL | Objective To evaluate the feasibility of Blocker PCR assays in monitoring T790M mutations in plasma of non-small-cell lung cancer (NSCLC) patients with epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) acquired resistance.Methods Blocker PCR assays were employed to identify mutations in plasma for 127 advanced NSCLC with acquired EGFR-TKI resistance.In addition,the paired tumor re-biopsy or PE samples were obtained to analyze EGFR mutations.Meanwhile,we evaluated the detection accuracy of Blocker PCR assays in comparison with the next generation sequencing (NGS).Results Among the 127 patients,40.15％ (51/127) EGFR T790M was detected in the plasma,78.44％ (40/51) coexisted with an EGFR activating mutation.Additionally,54.54 ％ (6/11) EGFR T790M was identified in re-biopsy tissues,while 43.75 ％ (14/32) were detected in the plasma.Furthermore,the concordance rate of Blocker PCR and NGS in identifying EGFR sensitizing mutations and EGFR T790M mutations was 100％.Conclusions Blocker PCR is a highly sensitive and reliable method in monitoring EGFR T790M mutations in the plasma of NSCLC patients with EGFR-TKI acquired resistance. |
| Author | 张美玲;李春;叶茂松;巩子英;张道允;张新 |
| AuthorAffiliation | 复旦大学附属中山医院呼吸内科,上海200032;上海允英医疗科技有限公司,上海201216 |
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| Author_FL | GONG Zi-ying ZHANG Xin ZHANG Dao-yun ZHANG Mei-ling YE Mao-song LI Chun |
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| DocumentTitleAlternate | Monitoring EGFR T790M mutations by Blocker PCR in plasma of advanced non-small-cell lung cancer patients with EGFR-TKI acquired resistance |
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| Keywords | 游离DNA EGFR T790M mutations Blocker PCR non-small-cell lung carcinoma EGFR T790M突变 非小细胞肺癌 cell free DNA |
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| Notes | ZHANG Mei-ling1, LI Chun1 , YE Mao-song1 , GONG Zi ying2 , ZHANG Dao-yun2 , ZHANG Xin1 （1 Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China ; 2. Shanghai Yunying Medical TeeD nology Co. Ltd, Shanghai 201216, China ） Objective To evaluate the feasibility of Blocker PCR assays in monitoring T790 M mutations in plasma of non-small-cell lung cancer（NSCLC）patients with epidermal growth factor receptor-tyrosine kinase inhibitor（EGFR-TKI）acquired resistance. Methods Blocker PCR assays were employed to identify mutations in plasma for 127 advanced NSCLC with acquired EGFR-TKI resistance.In addition,the paired tumor re-biopsy or PE samples were obtained to analyze EGFR mutations.Meanwhile,we evaluated the detection accuracy of Blocker PCR assays in comparison with the next generation sequencing（NGS）. Results Among the 127 patients,40.15%（51/127）EGFR T790 M was detected in the plasma,78.44%（40/51）coexisted with an EGFRactivating mutation.Additionally,54.54%（6/11）EGFR T790 M w |
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| PublicationYear | 2018 |
| Publisher | 复旦大学附属中山医院呼吸内科 上海200032%上海允英医疗科技有限公司 上海201216 |
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| Snippet | 目的探讨改良PCR（Blocker PCR）方法在晚期非小细胞肺癌（non-small-cell lung cancer,NSCLC）患者血浆游离DNA（cell free DNA,cfDNA）中检测继发EGFR T790 M突变的应用价... R563.9%R734.2; 目的 探讨改良PCR(Blocker PCR)方法在晚期非小细胞肺癌(non-small-cell lung cancer,NSCLC)患者血浆游离DNA(cell free DNA,cfDNA)中检测继发EGFR T790M突... |
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| SubjectTerms | Blocker EGFR PCR T790M突变 游离DNA 非小细胞肺癌 |
| Title | 改良Blocker PCR检测EGFR-TKI耐药后非小细胞肺癌血浆EGFR T790M突变的价值 |
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