丹酚酸B对人牙周膜细胞成骨分化的影响
目的 探讨中药丹参主要活性成分丹酚酸B（Sal B）对人牙周膜细胞（h PDLCs）成骨分化的影响。方法 取第3代h PDLCs进行实验,运用甲基噻唑基四唑（MTT）法检测不同浓度丹酚酸B对h PDLCs增殖活性影响,通过检测碱性磷酸酶（ALP）活性、矿化结节染色、骨钙素（OCN）m RNA表达来探讨丹酚酸B对h PDLCs成骨分化的影响。结果 丹酚酸B对h PDLCs增殖活性无影响。当丹酚酸B浓度为0.5、1、5μmol·L-1时均能增加h PDLCs的ALP活性和OCN m RNA表达,与OIM组比较差异有统计学意义（P〈0.05）;且当丹酚酸B浓度为0.5、1、5μmol·L-1时h P...
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| Published in | 华西口腔医学杂志 Vol. 34; no. 5; pp. 468 - 473 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
重庆医科大学附属口腔医院正畸科
2016
口腔疾病与生物医学重庆市重点实验室%重庆市高校市级口腔生物医学工程重点实验室,重庆,401147 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-1182 |
| DOI | 10.7518/hxkq.2016.05.007 |
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| Abstract | 目的 探讨中药丹参主要活性成分丹酚酸B（Sal B）对人牙周膜细胞（h PDLCs）成骨分化的影响。方法 取第3代h PDLCs进行实验,运用甲基噻唑基四唑（MTT）法检测不同浓度丹酚酸B对h PDLCs增殖活性影响,通过检测碱性磷酸酶（ALP）活性、矿化结节染色、骨钙素（OCN）m RNA表达来探讨丹酚酸B对h PDLCs成骨分化的影响。结果 丹酚酸B对h PDLCs增殖活性无影响。当丹酚酸B浓度为0.5、1、5μmol·L-1时均能增加h PDLCs的ALP活性和OCN m RNA表达,与OIM组比较差异有统计学意义（P〈0.05）;且当丹酚酸B浓度为0.5、1、5μmol·L-1时h PDLCs形成矿化结节数量明显高于OIM组。结论 适宜浓度的丹酚酸B能有效促进h PDLCs成骨分化。 |
|---|---|
| AbstractList | 目的 探讨中药丹参主要活性成分丹酚酸B（Sal B）对人牙周膜细胞（h PDLCs）成骨分化的影响。方法 取第3代h PDLCs进行实验,运用甲基噻唑基四唑（MTT）法检测不同浓度丹酚酸B对h PDLCs增殖活性影响,通过检测碱性磷酸酶（ALP）活性、矿化结节染色、骨钙素（OCN）m RNA表达来探讨丹酚酸B对h PDLCs成骨分化的影响。结果 丹酚酸B对h PDLCs增殖活性无影响。当丹酚酸B浓度为0.5、1、5μmol·L-1时均能增加h PDLCs的ALP活性和OCN m RNA表达,与OIM组比较差异有统计学意义（P〈0.05）;且当丹酚酸B浓度为0.5、1、5μmol·L-1时h PDLCs形成矿化结节数量明显高于OIM组。结论 适宜浓度的丹酚酸B能有效促进h PDLCs成骨分化。 R781.4; 目的 探讨中药丹参主要活性成分丹酚酸B (Sal B)对人牙周膜细胞(hPDLCs)成骨分化的影响.方法 取第3代hPDLCs进行实验,运用甲基噻唑基四唑(MTT)法检测不同浓度丹酚酸B对hPDLCs增殖活性影响,通过检测碱性磷酸酶(ALP)活性、矿化结节染色、骨钙素(OCN) mRNA表达来探讨丹酚酸B对hPDLCs成骨分化的影响.结果 丹酚酸B对hPDLCs增殖活性无影响.当丹酚酸B浓度为0.5、1、5μmol·L-1时均能增加hPDLCs的ALP活性和OCN mRNA表达,与OIM组比较差异有统计学意义(P＜0.05);且当丹酚酸B浓度为0.5、1、5μmol·L-1时hPDLCs形成矿化结节数量明显高于OIM组.结论 适宜浓度的丹酚酸B能有效促进hPDLCs成骨分化. |
| Author | 马玥 任嫒姝 付钢 |
| AuthorAffiliation | 重庆医科大学附属口腔医院正畸科 口腔疾病与生物医学重庆市重点实验室 重庆市高校市级口腔生物医学工程重点实验室,重庆401147 |
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| Author_FL | Ma Yue Ren Aishu Fu Gang |
| Author_FL_xml | – sequence: 1 fullname: Ma Yue – sequence: 2 fullname: Ren Aishu – sequence: 3 fullname: Fu Gang |
| Author_xml | – sequence: 1 fullname: 马玥 任嫒姝 付钢 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Effects of salvianolic acid B on osteogenic differentiation of human periodontal ligament cells |
| DocumentTitle_FL | Effects of salvianolic acid B on osteogenic differentiation of human periodontal ligament cells |
| EndPage | 473 |
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| Keywords | 碱性磷酸酶活性 human periodontal ligament cells 人牙周膜细胞 矿化结节 osteogenic differentiation mineralized nodules 丹酚酸B 成骨分化 salvianolic acid B alkaline phosphatase activity |
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| Notes | Objective This study investigated the effects of salvianolic acid B （Sal B）, a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells （hPDLCs）. Methods Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium （MTT） method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase （ALP） activity and mineralization capability were measured, and mRNA expression of osteocalcin （OCN） was detected to investigate the effects of Sal B on osteogenesis of hPDLCs. Results Sal B did not influence the viability ofhPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved （P〈0.05） under treatment with different Sal B concentrations （0.5, 1, and 5 μmol·L^-1） compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B co |
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| PublicationTitle | 华西口腔医学杂志 |
| PublicationTitleAlternate | West China Journal of Stomatology |
| PublicationTitle_FL | West China Journal of Stomatology |
| PublicationYear | 2016 |
| Publisher | 重庆医科大学附属口腔医院正畸科 口腔疾病与生物医学重庆市重点实验室%重庆市高校市级口腔生物医学工程重点实验室,重庆,401147 |
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| Snippet | 目的 探讨中药丹参主要活性成分丹酚酸B（Sal B）对人牙周膜细胞（h PDLCs）成骨分化的影响。方法 取第3代h PDLCs进行实验,运用甲基噻唑基四唑（MTT）法检测不同浓度丹酚酸B... R781.4; 目的 探讨中药丹参主要活性成分丹酚酸B (Sal B)对人牙周膜细胞(hPDLCs)成骨分化的影响.方法 取第3代hPDLCs进行实验,运用甲基噻唑基四唑(MTT)法检测不同浓度丹酚... |
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| SubjectTerms | 丹酚酸B 人牙周膜细胞 成骨分化 矿化结节 碱性磷酸酶活性 |
| Title | 丹酚酸B对人牙周膜细胞成骨分化的影响 |
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